Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-91 |
Sentence |
denotes |
On the interaction of alpha-lactalbumin and galactosyltransferase during lactose synthesis. |
T2 |
92-396 |
Sentence |
denotes |
The regulatory effect of alpha-lactalbumin in the lactose synthase system has been ascribed to its reversible association with a complex of galactosyltransferase with Mn2+ and UDP-galactose, prior to the binding of monosaccharides; the resulting complex has a higher affinity for various monosaccharides. |
T3 |
397-624 |
Sentence |
denotes |
Two steps in the postulated catalytic cycle have been investigated; UDP-galactose binding to enzyme-Mn2+ by equilibrium dialysis and alpha-lactalbumin binding to enzyme-Mn2+-UDP-galactose by sedimentation velocity and kinetics. |
T4 |
625-818 |
Sentence |
denotes |
There is a single binding site for UDP-galactose on the enzyme-Mn2+ complex, and the dissociation constant for UDP-galactose from enzyme-Mn2+-UDP-galactose was found to be 72 muM at 37 degrees. |
T5 |
819-1266 |
Sentence |
denotes |
The formation of a complex between galactosyltransferase and alpha-lactalbumin in the presence of Mn2+ and UDP-galactose was observed as an increase in sedimentation coefficient of enzyme activity So20,w from 3.25 +/- 0.03 in the absence of alpha-lactalbumin to 4.22 +/- 0.03 at saturating concentrations of alpha-lactalbumin, a value closely similar to that of a cross-linked 1:1 complex of the proteins under the same conditions (4.35 +/- 0.03). |
T6 |
1267-1355 |
Sentence |
denotes |
No interaction was observed in the absence of substrates or with UDP-galactose and EDTA. |
T7 |
1356-1583 |
Sentence |
denotes |
From the ultracentrifuge data and steady state kinetics, dissociation constants for alpha-lactalbumin from the enzyme-Mn2+-UDP-galactose-alpha-lactalbumin complex were determined at several temperatures and salt concentrations. |
T8 |
1584-1621 |
Sentence |
denotes |
These showed good internal agreement. |
T9 |
1622-1791 |
Sentence |
denotes |
The free energy change delta G degrees for the association of the two proteins is calculated, and the results are discussed in relation to the nature of the interaction. |
T1 |
0-91 |
Sentence |
denotes |
On the interaction of alpha-lactalbumin and galactosyltransferase during lactose synthesis. |
T2 |
92-396 |
Sentence |
denotes |
The regulatory effect of alpha-lactalbumin in the lactose synthase system has been ascribed to its reversible association with a complex of galactosyltransferase with Mn2+ and UDP-galactose, prior to the binding of monosaccharides; the resulting complex has a higher affinity for various monosaccharides. |
T3 |
397-624 |
Sentence |
denotes |
Two steps in the postulated catalytic cycle have been investigated; UDP-galactose binding to enzyme-Mn2+ by equilibrium dialysis and alpha-lactalbumin binding to enzyme-Mn2+-UDP-galactose by sedimentation velocity and kinetics. |
T4 |
625-818 |
Sentence |
denotes |
There is a single binding site for UDP-galactose on the enzyme-Mn2+ complex, and the dissociation constant for UDP-galactose from enzyme-Mn2+-UDP-galactose was found to be 72 muM at 37 degrees. |
T5 |
819-1266 |
Sentence |
denotes |
The formation of a complex between galactosyltransferase and alpha-lactalbumin in the presence of Mn2+ and UDP-galactose was observed as an increase in sedimentation coefficient of enzyme activity So20,w from 3.25 +/- 0.03 in the absence of alpha-lactalbumin to 4.22 +/- 0.03 at saturating concentrations of alpha-lactalbumin, a value closely similar to that of a cross-linked 1:1 complex of the proteins under the same conditions (4.35 +/- 0.03). |
T6 |
1267-1355 |
Sentence |
denotes |
No interaction was observed in the absence of substrates or with UDP-galactose and EDTA. |
T7 |
1356-1583 |
Sentence |
denotes |
From the ultracentrifuge data and steady state kinetics, dissociation constants for alpha-lactalbumin from the enzyme-Mn2+-UDP-galactose-alpha-lactalbumin complex were determined at several temperatures and salt concentrations. |
T8 |
1584-1621 |
Sentence |
denotes |
These showed good internal agreement. |
T9 |
1622-1791 |
Sentence |
denotes |
The free energy change delta G degrees for the association of the two proteins is calculated, and the results are discussed in relation to the nature of the interaction. |