| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-86 |
Sentence |
denotes |
Isolation of bacterial and phage proteins by homopolymer RNA-cellulose chromatography. |
| T2 |
87-280 |
Sentence |
denotes |
Nucleic acid-free extracts of Escherichia coli have been analyzed by chromatography on columns of cellulose, to which poly(A), poly(U), or poly(C) have been attached by ultraviolet irradiation. |
| T3 |
281-458 |
Sentence |
denotes |
Proteins are released from the columns by stepwise elution with increasingly higher concentrations of salt, followed by washing with urea to remove very tightly bound molecules. |
| T4 |
459-640 |
Sentence |
denotes |
The pattern of protein elution is reproducibly different for each of the homopolymer RNA-cellulose columns used: some proteins bind very tightly to one column, but poorly to others. |
| T5 |
641-868 |
Sentence |
denotes |
Analysis by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, by immunological cross-reactivity in double diffusion tests, and by enzymological assays, has allowed the identification of a number of these proteins. |
| T6 |
869-1021 |
Sentence |
denotes |
The RNA polymerase core enzyme binds to poly(C)- and to poly(U)-cellulose columns, and can be purified to 20 to 30 percent homogeneity in a single step. |
| T7 |
1022-1210 |
Sentence |
denotes |
Ribosomal protein S1 and the termination factor rho bind very tightly to poly(C)-cellulose, and both can be purified to homogeneity rapidly, in much higher yields than previously reported. |
| T8 |
1211-1366 |
Sentence |
denotes |
Poly(A)-cellulose chromatography allows the isolation of large amounts of an 80,000 molecular weight protein having an as yet unassigned cellular function. |
| T9 |
1367-1632 |
Sentence |
denotes |
The host factor required for RNA phage Qbeta RNA replication in vitro can also be obtained from poly(A)-cellulose, and chromatography of extracts of phage Qbeta-infected E. coli on RNA-cellulose columns results in very rapid isolation of the Qbeta replicase enzyme. |
| T10 |
1633-1794 |
Sentence |
denotes |
Homopolymer RNA-cellulose chromatography thus appears to be a simple, general technique, useful for the efficient isolation of a variety of RNA-binding proteins. |
| T1 |
0-86 |
Sentence |
denotes |
Isolation of bacterial and phage proteins by homopolymer RNA-cellulose chromatography. |
| T2 |
87-280 |
Sentence |
denotes |
Nucleic acid-free extracts of Escherichia coli have been analyzed by chromatography on columns of cellulose, to which poly(A), poly(U), or poly(C) have been attached by ultraviolet irradiation. |
| T3 |
281-458 |
Sentence |
denotes |
Proteins are released from the columns by stepwise elution with increasingly higher concentrations of salt, followed by washing with urea to remove very tightly bound molecules. |
| T4 |
459-640 |
Sentence |
denotes |
The pattern of protein elution is reproducibly different for each of the homopolymer RNA-cellulose columns used: some proteins bind very tightly to one column, but poorly to others. |
| T5 |
641-868 |
Sentence |
denotes |
Analysis by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, by immunological cross-reactivity in double diffusion tests, and by enzymological assays, has allowed the identification of a number of these proteins. |
| T6 |
869-1021 |
Sentence |
denotes |
The RNA polymerase core enzyme binds to poly(C)- and to poly(U)-cellulose columns, and can be purified to 20 to 30 percent homogeneity in a single step. |
| T7 |
1022-1210 |
Sentence |
denotes |
Ribosomal protein S1 and the termination factor rho bind very tightly to poly(C)-cellulose, and both can be purified to homogeneity rapidly, in much higher yields than previously reported. |
| T8 |
1211-1366 |
Sentence |
denotes |
Poly(A)-cellulose chromatography allows the isolation of large amounts of an 80,000 molecular weight protein having an as yet unassigned cellular function. |
| T9 |
1367-1632 |
Sentence |
denotes |
The host factor required for RNA phage Qbeta RNA replication in vitro can also be obtained from poly(A)-cellulose, and chromatography of extracts of phage Qbeta-infected E. coli on RNA-cellulose columns results in very rapid isolation of the Qbeta replicase enzyme. |
| T10 |
1633-1794 |
Sentence |
denotes |
Homopolymer RNA-cellulose chromatography thus appears to be a simple, general technique, useful for the efficient isolation of a variety of RNA-binding proteins. |
