PubMed:7935795 JSONTXT

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":14,"end":19},"obj":"protein"},{"id":"T2","span":{"begin":52,"end":57},"obj":"protein"},{"id":"T3","span":{"begin":126,"end":132},"obj":"protein"},{"id":"T4","span":{"begin":212,"end":217},"obj":"protein"},{"id":"T5","span":{"begin":373,"end":378},"obj":"protein"},{"id":"T6","span":{"begin":396,"end":407},"obj":"protein"},{"id":"T7","span":{"begin":409,"end":413},"obj":"protein"},{"id":"T8","span":{"begin":419,"end":430},"obj":"protein"},{"id":"T9","span":{"begin":432,"end":435},"obj":"protein"},{"id":"T10","span":{"begin":542,"end":547},"obj":"protein"},{"id":"T11","span":{"begin":570,"end":575},"obj":"protein"},{"id":"T12","span":{"begin":635,"end":640},"obj":"protein"}],"text":"Activation of Raf-1 by 14-3-3 proteins.\nThe protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein."}