PubMed:7926727 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/7926727","sourcedb":"PubMed","sourceid":"7926727","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/7926727","text":"Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein.\nThe p53 tumor suppressor gene product is a transcriptional activator that may be associated with its ability to suppress tumor cell growth. The acidic amino terminus of the p53 protein has been shown to contain this trans-activation activity as well as the domains for mdm-2 and adenovirus 5 E1B 55-kD protein binding. An extensive genetic analysis of this amino-terminal p53 domain has been undertaken using site-specific mutagenesis. The results demonstrate that the acidic residues in the amino terminus of p53 may contribute to, but are not critical for, this trans-activation activity. Rather, the hydrophobic amino acid residues Leu-22 and Trp-23 of human p53 are both required for trans-activation activity, binding to the adenovirus E1B 55-kD protein and the human mdm-2-p53 protein in vitro. In addition, hydrophobic residues Leu-14 and Phe-19 are crucial for the interactions between p53 and human mdm-2 (hdm-2). Hydrophobic residues Trp-23 and Pro-27 are also important for binding to the adenovirus 5 (Ad5) E1B 55-kD protein in vitro. These mutations have no impact on the ability of the p53 protein to bind to a p53-specific DNA element. These results suggest that 2-4 critical hydrophobic residues in the amino-terminal domain of the p53 protein interact with the transcriptional machinery of the cell resulting in transcriptional activation. These very same hydrophobic residues contact the hdm-2 and Ad5 E1B 55-kD oncogene products.","tracks":[{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":39,"end":42},"obj":"protein"},{"id":"T2","span":{"begin":121,"end":126},"obj":"protein"},{"id":"T3","span":{"begin":148,"end":151},"obj":"protein"},{"id":"T4","span":{"begin":171,"end":174},"obj":"protein"},{"id":"T5","span":{"begin":340,"end":343},"obj":"protein"},{"id":"T6","span":{"begin":436,"end":441},"obj":"protein"},{"id":"T7","span":{"begin":459,"end":462},"obj":"protein"},{"id":"T8","span":{"begin":539,"end":542},"obj":"protein"},{"id":"T9","span":{"begin":677,"end":680},"obj":"protein"},{"id":"T10","span":{"begin":829,"end":832},"obj":"protein"},{"id":"T11","span":{"begin":908,"end":911},"obj":"protein"},{"id":"T12","span":{"begin":940,"end":945},"obj":"protein"},{"id":"T13","span":{"begin":946,"end":949},"obj":"protein"},{"id":"T14","span":{"begin":1061,"end":1064},"obj":"protein"},{"id":"T15","span":{"begin":1075,"end":1080},"obj":"protein"},{"id":"T16","span":{"begin":1082,"end":1087},"obj":"protein"},{"id":"T17","span":{"begin":1186,"end":1189},"obj":"protein"},{"id":"T18","span":{"begin":1267,"end":1270},"obj":"protein"},{"id":"T19","span":{"begin":1292,"end":1295},"obj":"protein"},{"id":"T20","span":{"begin":1415,"end":1418},"obj":"protein"},{"id":"T21","span":{"begin":1573,"end":1578},"obj":"protein"},{"id":"T22","span":{"begin":1587,"end":1590},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"AIMed"},{"subj":"T2","pred":"source","obj":"AIMed"},{"subj":"T3","pred":"source","obj":"AIMed"},{"subj":"T4","pred":"source","obj":"AIMed"},{"subj":"T5","pred":"source","obj":"AIMed"},{"subj":"T6","pred":"source","obj":"AIMed"},{"subj":"T7","pred":"source","obj":"AIMed"},{"subj":"T8","pred":"source","obj":"AIMed"},{"subj":"T9","pred":"source","obj":"AIMed"},{"subj":"T10","pred":"source","obj":"AIMed"},{"subj":"T11","pred":"source","obj":"AIMed"},{"subj":"T12","pred":"source","obj":"AIMed"},{"subj":"T13","pred":"source","obj":"AIMed"},{"subj":"T14","pred":"source","obj":"AIMed"},{"subj":"T15","pred":"source","obj":"AIMed"},{"subj":"T16","pred":"source","obj":"AIMed"},{"subj":"T17","pred":"source","obj":"AIMed"},{"subj":"T18","pred":"source","obj":"AIMed"},{"subj":"T19","pred":"source","obj":"AIMed"},{"subj":"T20","pred":"source","obj":"AIMed"},{"subj":"T21","pred":"source","obj":"AIMed"},{"subj":"T22","pred":"source","obj":"AIMed"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AIMed","color":"#93ecb1","default":true}]}]}}