| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-73 |
Sentence |
denotes |
Specific binding of the transglutaminase, platelet factor XIII, to HSP27. |
| T2 |
74-312 |
Sentence |
denotes |
HSP27, the unique mammalian low molecular weight heat shock protein, is prominently phosphorylated upon activation of a wide variety of cells and has a role in thermotolerance, growth events, and regulation of actin cytoskeletal dynamics. |
| T3 |
313-449 |
Sentence |
denotes |
In thrombin-stimulated platelets, HSP27 is rapidly and prominently phosphorylated in a manner highly correlated with platelet secretion. |
| T4 |
450-550 |
Sentence |
denotes |
However, the function of HSP27 and the identity of proteins that interact with HSP27 remain unknown. |
| T5 |
551-769 |
Sentence |
denotes |
To identify specific HSP27-protein interactions, a recombinant fusion protein affinity reagent was constructed and used to identify proteins associating with HSP27 from human platelet lysates and erythroleukemia cells. |
| T6 |
770-989 |
Sentence |
denotes |
An 84-kDa protein was found to associate specifically with HSP27 and was isolated from platelet lysates, resolved on preparative gels, transferred to nitrocellulose, subjected to enzymatic digestion, and microsequenced. |
| T7 |
990-1118 |
Sentence |
denotes |
A 20-amino acid sequence derived from p84 proved identical to amino acids 484-503 of the transglutaminase, platelet Factor XIII. |
| T8 |
1119-1240 |
Sentence |
denotes |
Immunoblotting studies were used to confirm the binding of FXIII from fresh platelet lysates to the HSP27 fusion protein. |
| T9 |
1241-1416 |
Sentence |
denotes |
FXIII also was shown to coprecipitate with HSP27 in immunoprecipitation studies and to colocalize with HSP27 in immunofluorescence studies of intact glass-activated platelets. |
| T10 |
1417-1601 |
Sentence |
denotes |
The data thus demonstrate specific binding of platelet FXIII to HSP27 and suggest that HSP27 may participate in the cellular localization and/or enzymatic regulation of platelet FXIII. |
| T1 |
0-73 |
Sentence |
denotes |
Specific binding of the transglutaminase, platelet factor XIII, to HSP27. |
| T2 |
74-312 |
Sentence |
denotes |
HSP27, the unique mammalian low molecular weight heat shock protein, is prominently phosphorylated upon activation of a wide variety of cells and has a role in thermotolerance, growth events, and regulation of actin cytoskeletal dynamics. |
| T3 |
313-449 |
Sentence |
denotes |
In thrombin-stimulated platelets, HSP27 is rapidly and prominently phosphorylated in a manner highly correlated with platelet secretion. |
| T4 |
450-550 |
Sentence |
denotes |
However, the function of HSP27 and the identity of proteins that interact with HSP27 remain unknown. |
| T5 |
551-769 |
Sentence |
denotes |
To identify specific HSP27-protein interactions, a recombinant fusion protein affinity reagent was constructed and used to identify proteins associating with HSP27 from human platelet lysates and erythroleukemia cells. |
| T6 |
770-989 |
Sentence |
denotes |
An 84-kDa protein was found to associate specifically with HSP27 and was isolated from platelet lysates, resolved on preparative gels, transferred to nitrocellulose, subjected to enzymatic digestion, and microsequenced. |
| T7 |
990-1118 |
Sentence |
denotes |
A 20-amino acid sequence derived from p84 proved identical to amino acids 484-503 of the transglutaminase, platelet Factor XIII. |
| T8 |
1119-1240 |
Sentence |
denotes |
Immunoblotting studies were used to confirm the binding of FXIII from fresh platelet lysates to the HSP27 fusion protein. |
| T9 |
1241-1416 |
Sentence |
denotes |
FXIII also was shown to coprecipitate with HSP27 in immunoprecipitation studies and to colocalize with HSP27 in immunofluorescence studies of intact glass-activated platelets. |
| T10 |
1417-1601 |
Sentence |
denotes |
The data thus demonstrate specific binding of platelet FXIII to HSP27 and suggest that HSP27 may participate in the cellular localization and/or enzymatic regulation of platelet FXIII. |