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PubMed:7829531 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-213 Sentence denotes Mutagenesis of the regulatory subunit (RII beta) of cAMP-dependent protein kinase II beta reveals hydrophobic amino acids that are essential for RII beta dimerization and/or anchoring RII beta to the cytoskeleton.
T2 214-430 Sentence denotes In neurons cAMP-dependent protein kinase II beta (PKAII beta) is sequestered in the dendritic cytoskeleton because the regulatory subunit (RII beta) of the enzyme is tightly bound by A Kinase Anchor Proteins (AKAPs).
T3 431-542 Sentence denotes The prototypic neuronal anchor protein AKAP75 has a COOH-terminal 22-residue RII beta binding (tethering) site.
T4 543-686 Sentence denotes A key feature of the tethering site is that several amino acids with large aliphatic side chains mediate the high-affinity binding of RII beta.
T5 687-882 Sentence denotes Mutagenesis, recombinant protein expression, and physicochemical characterization were used to investigate the structural basis for the homodimerization and AKAP75 binding activities of RII beta.
T6 883-981 Sentence denotes Several crucial residues are located in an NH2-terminal region that encompasses amino acids 13-36.
T7 982-1083 Sentence denotes Substitution of Ala for Leu13 or Phe36 generates monomeric RII beta subunits that cannot bind AKAP75.
T8 1084-1284 Sentence denotes The results are not due to general misfolding since mutant RII beta monomers bind cAMP and inhibit the catalytic subunit of PKAII beta with the same affinity and efficacy as wild-type RII beta dimers.
T9 1285-1445 Sentence denotes Moreover, substitution of Ala for Leu12, Val20, Leu21, Phe31, Leu33, or Leu39 and replacement of Leu13 with Ile or Val did not impair the dimerization reaction.
T10 1446-1571 Sentence denotes Evidently, large hydrophobic side chains of Leu13 and Phe36 play pivotal roles in stabilizing RII beta-RII beta interactions.
T11 1572-1724 Sentence denotes A secondary consequence of destabilizing RII beta dimers is the loss of intracellular targeting/anchoring capacity because monomers fail to bind AKAP75.
T12 1725-1833 Sentence denotes Other NH2-terminal residues directly modulate the affinity of RII beta dimers for the AKAP75 tethering site.
T13 1834-1977 Sentence denotes Replacement of Val20-Leu21 with Ala-Ala produced a dimeric RII beta protein that binds AKAP75 approximately 4% as avidly as wild-type RII beta.
T14 1978-2123 Sentence denotes It is possible that the aliphatic side chains of Val20 and Leu21 interact with the essential Leu and Ile residues in the AKAP75 tethering region.
T1 0-213 Sentence denotes Mutagenesis of the regulatory subunit (RII beta) of cAMP-dependent protein kinase II beta reveals hydrophobic amino acids that are essential for RII beta dimerization and/or anchoring RII beta to the cytoskeleton.
T2 214-430 Sentence denotes In neurons cAMP-dependent protein kinase II beta (PKAII beta) is sequestered in the dendritic cytoskeleton because the regulatory subunit (RII beta) of the enzyme is tightly bound by A Kinase Anchor Proteins (AKAPs).
T3 431-542 Sentence denotes The prototypic neuronal anchor protein AKAP75 has a COOH-terminal 22-residue RII beta binding (tethering) site.
T4 543-686 Sentence denotes A key feature of the tethering site is that several amino acids with large aliphatic side chains mediate the high-affinity binding of RII beta.
T5 687-882 Sentence denotes Mutagenesis, recombinant protein expression, and physicochemical characterization were used to investigate the structural basis for the homodimerization and AKAP75 binding activities of RII beta.
T6 883-981 Sentence denotes Several crucial residues are located in an NH2-terminal region that encompasses amino acids 13-36.
T7 982-1083 Sentence denotes Substitution of Ala for Leu13 or Phe36 generates monomeric RII beta subunits that cannot bind AKAP75.
T8 1084-1284 Sentence denotes The results are not due to general misfolding since mutant RII beta monomers bind cAMP and inhibit the catalytic subunit of PKAII beta with the same affinity and efficacy as wild-type RII beta dimers.
T9 1285-1445 Sentence denotes Moreover, substitution of Ala for Leu12, Val20, Leu21, Phe31, Leu33, or Leu39 and replacement of Leu13 with Ile or Val did not impair the dimerization reaction.
T10 1446-1571 Sentence denotes Evidently, large hydrophobic side chains of Leu13 and Phe36 play pivotal roles in stabilizing RII beta-RII beta interactions.
T11 1572-1724 Sentence denotes A secondary consequence of destabilizing RII beta dimers is the loss of intracellular targeting/anchoring capacity because monomers fail to bind AKAP75.
T12 1725-1833 Sentence denotes Other NH2-terminal residues directly modulate the affinity of RII beta dimers for the AKAP75 tethering site.
T13 1834-1977 Sentence denotes Replacement of Val20-Leu21 with Ala-Ala produced a dimeric RII beta protein that binds AKAP75 approximately 4% as avidly as wild-type RII beta.
T14 1978-2123 Sentence denotes It is possible that the aliphatic side chains of Val20 and Leu21 interact with the essential Leu and Ile residues in the AKAP75 tethering region.

AIMed

Id Subject Object Predicate Lexical cue
T1 39-47 protein denotes RII beta
T2 52-89 protein denotes cAMP-dependent protein kinase II beta
T3 145-153 protein denotes RII beta
T4 184-192 protein denotes RII beta
T5 225-262 protein denotes cAMP-dependent protein kinase II beta
T6 264-274 protein denotes PKAII beta
T7 353-361 protein denotes RII beta
T8 470-476 protein denotes AKAP75
T9 508-516 protein denotes RII beta
T10 677-685 protein denotes RII beta
T11 844-850 protein denotes AKAP75
T12 873-881 protein denotes RII beta
T13 1041-1049 protein denotes RII beta
T14 1076-1082 protein denotes AKAP75
T15 1143-1151 protein denotes RII beta
T16 1208-1218 protein denotes PKAII beta
T17 1268-1276 protein denotes RII beta
T18 1540-1548 protein denotes RII beta
T19 1549-1557 protein denotes RII beta
T20 1613-1621 protein denotes RII beta
T21 1717-1723 protein denotes AKAP75
T22 1787-1795 protein denotes RII beta
T23 1811-1817 protein denotes AKAP75
T24 1893-1901 protein denotes RII beta
T25 1921-1927 protein denotes AKAP75
T26 1968-1976 protein denotes RII beta
T27 2099-2105 protein denotes AKAP75

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 200-212 Body_part denotes cytoskeleton http://purl.obolibrary.org/obo/GO_0005856
T2 308-320 Body_part denotes cytoskeleton http://purl.obolibrary.org/obo/GO_0005856
T3 1644-1657 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 217-224 Cell denotes neurons http://purl.obolibrary.org/obo/CL:0000540
T2 446-454 Cell denotes neuronal http://purl.obolibrary.org/obo/CL:0000540