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sentences

Id Subject Object Predicate Lexical cue
T1 0-70 Sentence denotes Structural analysis of myosin heavy chain kinase A from Dictyostelium.
T2 71-237 Sentence denotes Evidence for a highly divergent protein kinase domain, an amino-terminal coiled-coil domain, and a domain homologous to the beta-subunit of heterotrimeric G proteins.
T3 238-384 Sentence denotes We report here the cloning and characterization of the gene encoding the 130-kDa myosin heavy chain kinase (MHCK A) from the amoeba Dictyostelium.
T4 385-680 Sentence denotes Previous studies have shown that purified MHCK A phosphorylates threonines in the carboxyl-terminal tail portion of the Dictyostelium myosin II heavy chain and that phosphorylation of these sites is critical in regulating the assembly and disassembly of myosin II filaments in vitro and in vivo.
T5 681-1046 Sentence denotes Biochemical analysis of MHCK A, together with analysis of the primary sequence, suggests that the amino-terminal approximately 500 amino acids form an alpha-helical coiled-coil domain and that residues from position approximately 860 to the carboxyl terminus (residue 1146) form a domain with significant similarity to the beta-subunit of heterotrimeric G proteins.
T6 1047-1177 Sentence denotes No part of the MHCK A sequence displays significant similarity to the catalytic domain of conventional eukaryotic protein kinases.
T7 1178-1439 Sentence denotes However, both native and recombinant MHCK A displayed autophosphorylation activity following renaturation from SDS gels, and MHCK A expressed in Escherichia coli phosphorylated purified Dictyostelium myosin, confirming that MHCK A is a bona fide protein kinase.
T8 1440-1578 Sentence denotes Cross-linking studies demonstrated that native MHCK A is a multimer, consistent with the presence of an amino-terminal coiled-coil domain.
T9 1579-1683 Sentence denotes Southern blot analysis indicates that MHCK A is encoded by a single gene that has no detectable introns.
T1 0-70 Sentence denotes Structural analysis of myosin heavy chain kinase A from Dictyostelium.
T2 71-237 Sentence denotes Evidence for a highly divergent protein kinase domain, an amino-terminal coiled-coil domain, and a domain homologous to the beta-subunit of heterotrimeric G proteins.
T3 238-384 Sentence denotes We report here the cloning and characterization of the gene encoding the 130-kDa myosin heavy chain kinase (MHCK A) from the amoeba Dictyostelium.
T4 385-680 Sentence denotes Previous studies have shown that purified MHCK A phosphorylates threonines in the carboxyl-terminal tail portion of the Dictyostelium myosin II heavy chain and that phosphorylation of these sites is critical in regulating the assembly and disassembly of myosin II filaments in vitro and in vivo.
T5 681-1046 Sentence denotes Biochemical analysis of MHCK A, together with analysis of the primary sequence, suggests that the amino-terminal approximately 500 amino acids form an alpha-helical coiled-coil domain and that residues from position approximately 860 to the carboxyl terminus (residue 1146) form a domain with significant similarity to the beta-subunit of heterotrimeric G proteins.
T6 1047-1177 Sentence denotes No part of the MHCK A sequence displays significant similarity to the catalytic domain of conventional eukaryotic protein kinases.
T7 1178-1439 Sentence denotes However, both native and recombinant MHCK A displayed autophosphorylation activity following renaturation from SDS gels, and MHCK A expressed in Escherichia coli phosphorylated purified Dictyostelium myosin, confirming that MHCK A is a bona fide protein kinase.
T8 1440-1578 Sentence denotes Cross-linking studies demonstrated that native MHCK A is a multimer, consistent with the presence of an amino-terminal coiled-coil domain.
T9 1579-1683 Sentence denotes Southern blot analysis indicates that MHCK A is encoded by a single gene that has no detectable introns.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 56-69 OrganismTaxon denotes Dictyostelium 5782
T2 363-369 OrganismTaxon denotes amoeba 5774
T3 370-383 OrganismTaxon denotes Dictyostelium 5782
T4 505-518 OrganismTaxon denotes Dictyostelium 5782
T5 1323-1339 OrganismTaxon denotes Escherichia coli 562
T6 1364-1377 OrganismTaxon denotes Dictyostelium 5782

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 485-489 Body_part denotes tail http://purl.obolibrary.org/obo/UBERON_0002415|http://purl.obolibrary.org/obo/UBERON_4000164