PubMed:7816143 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/7816143","sourcedb":"PubMed","sourceid":"7816143","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/7816143","text":"Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA.\nThe Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous alpha-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.","tracks":[{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":65,"end":70},"obj":"protein"},{"id":"T2","span":{"begin":71,"end":76},"obj":"protein"},{"id":"T3","span":{"begin":95,"end":98},"obj":"protein"},{"id":"T4","span":{"begin":103,"end":106},"obj":"protein"},{"id":"T5","span":{"begin":319,"end":324},"obj":"protein"},{"id":"T6","span":{"begin":329,"end":334},"obj":"protein"},{"id":"T7","span":{"begin":697,"end":700},"obj":"protein"},{"id":"T8","span":{"begin":701,"end":704},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"AIMed"},{"subj":"T2","pred":"source","obj":"AIMed"},{"subj":"T3","pred":"source","obj":"AIMed"},{"subj":"T4","pred":"source","obj":"AIMed"},{"subj":"T5","pred":"source","obj":"AIMed"},{"subj":"T6","pred":"source","obj":"AIMed"},{"subj":"T7","pred":"source","obj":"AIMed"},{"subj":"T8","pred":"source","obj":"AIMed"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AIMed","color":"#abec93","default":true}]}]}}