> top > docs > PubMed:7802263 > annotations

PubMed:7802263 JSONTXT

Annnotations TAB JSON ListView MergeView

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 16-23 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T2 115-122 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T3 318-325 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T4 597-604 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T5 861-868 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T6 953-960 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 16-23 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T2 115-122 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T3 318-325 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T4 597-604 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T5 861-868 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T6 953-960 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-77 Sentence denotes Engineering the maltose binding protein for reagentless fluorescence sensing.
T2 78-255 Sentence denotes This paper describes a mutant of the maltose binding protein (MBP) in which the serine residue at position 337 is replaced by a cysteine residue using site-directed mutagenesis.
T3 256-511 Sentence denotes The mutant MBP has an approximately 2-fold lower affinity for maltose, and the cysteine residue can be modified with 4-[N-(2-(iodoacetoxy)ethyl)-N-methylamino]-7-nitrobenz-2-oxa-1,3-diazole (IANBD) and 6-acryloyl-2-(dimethylamino)-naphthalene (acrylodan).
T4 512-749 Sentence denotes This combined genetic and chemical modification places the fluorophores close to the maltose binding site such that when the ligand is added the fluorescence intensity of the labels increases by 60-180% over that of the ligand-free form.
T5 750-877 Sentence denotes This change is consistent with the fluorophores being buried when the conformation of the protein changes with maltose binding.
T6 878-1054 Sentence denotes Titration of the labeled mutant proteins yields dissociation constants for maltose of 62 +/- 0.2 and 0.8 +/- 0.01 microM respectively for the IANBD and acrylodan modifications.
T7 1055-1202 Sentence denotes The application of this strategy of combined genetic and chemical modification to the development of reagentless fluorescence sensing is discussed.