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PubMed:7782277 JSONTXT

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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 1522-1529 Cell denotes T cells http://purl.obolibrary.org/obo/CL:0000084

sentences

Id Subject Object Predicate Lexical cue
T1 0-88 Sentence denotes A direct interaction between G-protein beta gamma subunits and the Raf-1 protein kinase.
T2 89-209 Sentence denotes Raf-1 is a serine/threonine protein kinase positioned downstream of Ras in the mitogen-activated protein kinase cascade.
T3 210-431 Sentence denotes Using a yeast two-hybrid strategy to identify other proteins that interact with and potentially regulate Raf-1, we isolated a clone encoding the carboxyl-terminal half of the G beta 2 subunit of heterotrimeric G-proteins.
T4 432-562 Sentence denotes In vitro, purified G beta gamma subunits specifically bound to a GST fusion protein encoding amino acids 1-330 of Raf-1 (Raf/330).
T5 563-737 Sentence denotes Binding assays with truncation mutants of GST-Raf indicate that the region located between amino acids 136 and 239 is a primary determinant for interaction with G beta gamma.
T6 738-949 Sentence denotes In competition experiments, the carboxyl terminus of beta-adrenergic receptor kinase (beta ARK) blocked the binding of G beta gamma to Raf/330; however, the Raf-1-binding proteins, Ras and 14-3-3, had no effect.
T7 950-1155 Sentence denotes Scatchard analysis of in vitro binding between Raf/330 and G beta gamma revealed an affinity of interaction (Kd = 163 +/- 36 nM), similar to that seen between G beta gamma and beta ARK (Kd = 87 +/- 24 nM).
T8 1156-1431 Sentence denotes The formation of native heterotrimeric G alpha beta gamma complexes, as measured by pertussis toxin ADP-ribosylation of G alpha, could be disrupted by increasing amounts of Raf/330, with an EC50 of approximately 200 nM, in close agreement with the estimated binding affinity.
T9 1432-1571 Sentence denotes In vivo complexes of Raf-1 and G beta gamma were isolated from human embryonic kidney 293-T cells transfected with epitope-tagged G beta 2.
T10 1572-1781 Sentence denotes The identification and characterization of this novel interaction raises several possibilities for signaling cross-talk between growth factor receptors and those receptors coupled to heterotrimeric G-proteins.
T1 0-88 Sentence denotes A direct interaction between G-protein beta gamma subunits and the Raf-1 protein kinase.
T2 89-209 Sentence denotes Raf-1 is a serine/threonine protein kinase positioned downstream of Ras in the mitogen-activated protein kinase cascade.
T3 210-431 Sentence denotes Using a yeast two-hybrid strategy to identify other proteins that interact with and potentially regulate Raf-1, we isolated a clone encoding the carboxyl-terminal half of the G beta 2 subunit of heterotrimeric G-proteins.
T4 432-562 Sentence denotes In vitro, purified G beta gamma subunits specifically bound to a GST fusion protein encoding amino acids 1-330 of Raf-1 (Raf/330).
T5 563-737 Sentence denotes Binding assays with truncation mutants of GST-Raf indicate that the region located between amino acids 136 and 239 is a primary determinant for interaction with G beta gamma.
T6 738-949 Sentence denotes In competition experiments, the carboxyl terminus of beta-adrenergic receptor kinase (beta ARK) blocked the binding of G beta gamma to Raf/330; however, the Raf-1-binding proteins, Ras and 14-3-3, had no effect.
T7 950-1155 Sentence denotes Scatchard analysis of in vitro binding between Raf/330 and G beta gamma revealed an affinity of interaction (Kd = 163 +/- 36 nM), similar to that seen between G beta gamma and beta ARK (Kd = 87 +/- 24 nM).
T8 1156-1431 Sentence denotes The formation of native heterotrimeric G alpha beta gamma complexes, as measured by pertussis toxin ADP-ribosylation of G alpha, could be disrupted by increasing amounts of Raf/330, with an EC50 of approximately 200 nM, in close agreement with the estimated binding affinity.
T9 1432-1571 Sentence denotes In vivo complexes of Raf-1 and G beta gamma were isolated from human embryonic kidney 293-T cells transfected with epitope-tagged G beta 2.
T10 1572-1781 Sentence denotes The identification and characterization of this novel interaction raises several possibilities for signaling cross-talk between growth factor receptors and those receptors coupled to heterotrimeric G-proteins.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 1511-1517 http://purl.obolibrary.org/obo/MAT_0000119 denotes kidney

AIMed

Id Subject Object Predicate Lexical cue
T1 29-49 protein denotes G-protein beta gamma
T2 67-87 protein denotes Raf-1 protein kinase
T3 89-94 protein denotes Raf-1
T4 157-160 protein denotes Ras
T5 168-200 protein denotes mitogen-activated protein kinase
T6 315-320 protein denotes Raf-1
T7 385-401 protein denotes G beta 2 subunit
T8 451-463 protein denotes G beta gamma
T9 546-551 protein denotes Raf-1
T10 553-560 protein denotes Raf/330
T11 609-612 protein denotes Raf
T12 724-736 protein denotes G beta gamma
T13 791-822 protein denotes beta-adrenergic receptor kinase
T14 824-832 protein denotes beta ARK
T15 857-869 protein denotes G beta gamma
T16 873-880 protein denotes Raf/330
T17 895-900 protein denotes Raf-1
T18 919-922 protein denotes Ras
T19 997-1004 protein denotes Raf/330
T20 1009-1021 protein denotes G beta gamma
T21 1109-1121 protein denotes G beta gamma
T22 1126-1134 protein denotes beta ARK
T23 1195-1213 protein denotes G alpha beta gamma
T24 1329-1336 protein denotes Raf/330
T25 1453-1458 protein denotes Raf-1
T26 1463-1475 protein denotes G beta gamma
T27 1562-1570 protein denotes G beta 2

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 1511-1517 Body_part denotes kidney http://purl.obolibrary.org/obo/MAT_0000119

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 1240-1249 Disease denotes pertussis http://purl.obolibrary.org/obo/MONDO_0005077

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1495-1500 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1501-1517 Body_part denotes embryonic kidney http://purl.obolibrary.org/obo/UBERON_0002120