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PubMed:7772866 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-105 Sentence denotes Location of the active site of the bean alpha-amylase inhibitor and involvement of a Trp, Arg, Tyr triad.
TextSentencer_T2 106-274 Sentence denotes Seeds of the common bean contain three homologous proteins: phytohaemagglutinin E, phytohaemagglutinin L and the lectin-like protein alpha-amylase inhibitor (alpha AI).
TextSentencer_T3 275-638 Sentence denotes Whereas the active site of lectins has been studied in great detail, there is no information on the active site of the related protein alpha AI, which exerts its biological activity by making a 1:1 complex with alpha-amylase. alpha-amylase inhibitor is synthesized as a 30 kDa precursor glycoprotein that needs to be processed at Asn77 to form an active molecule.
TextSentencer_T4 639-836 Sentence denotes Comparison of the amino acid sequence of the bean alpha AI with that of the bacterial amylase inhibitor, tendamistat, suggested that a region around Trp188 might be involved in the inhibitory site.
TextSentencer_T5 837-986 Sentence denotes When a three-dimensional model of the bean alpha AI was constructed based on its homology to the legume lectins, this Trp region was alongside Asn77.
TextSentencer_T6 987-1127 Sentence denotes To test this site hypothesis, mutants of alpha AI were created by site-directed mutagenesis of the cDNA and expressed in transgenic tobacco.
TextSentencer_T7 1128-1230 Sentence denotes The mutant proteins R74N and WSY188-190GNV, as well as the double mutant, were inactive as inhibitors.
TextSentencer_T8 1231-1483 Sentence denotes These findings suggest that the active site of alpha AI consists of W188, R74 and Y190, in analogy to the Trp-Arg-Tyr motif of tendamistat, and that the processing of the polypeptide at Asn77 may be necessary to bring these residues in close proximity.
T1 0-105 Sentence denotes Location of the active site of the bean alpha-amylase inhibitor and involvement of a Trp, Arg, Tyr triad.
T2 106-274 Sentence denotes Seeds of the common bean contain three homologous proteins: phytohaemagglutinin E, phytohaemagglutinin L and the lectin-like protein alpha-amylase inhibitor (alpha AI).
T3 275-638 Sentence denotes Whereas the active site of lectins has been studied in great detail, there is no information on the active site of the related protein alpha AI, which exerts its biological activity by making a 1:1 complex with alpha-amylase. alpha-amylase inhibitor is synthesized as a 30 kDa precursor glycoprotein that needs to be processed at Asn77 to form an active molecule.
T4 639-836 Sentence denotes Comparison of the amino acid sequence of the bean alpha AI with that of the bacterial amylase inhibitor, tendamistat, suggested that a region around Trp188 might be involved in the inhibitory site.
T5 837-986 Sentence denotes When a three-dimensional model of the bean alpha AI was constructed based on its homology to the legume lectins, this Trp region was alongside Asn77.
T6 987-1127 Sentence denotes To test this site hypothesis, mutants of alpha AI were created by site-directed mutagenesis of the cDNA and expressed in transgenic tobacco.
T7 1128-1230 Sentence denotes The mutant proteins R74N and WSY188-190GNV, as well as the double mutant, were inactive as inhibitors.
T8 1231-1483 Sentence denotes These findings suggest that the active site of alpha AI consists of W188, R74 and Y190, in analogy to the Trp-Arg-Tyr motif of tendamistat, and that the processing of the polypeptide at Asn77 may be necessary to bring these residues in close proximity.
T1 0-105 Sentence denotes Location of the active site of the bean alpha-amylase inhibitor and involvement of a Trp, Arg, Tyr triad.
T2 106-274 Sentence denotes Seeds of the common bean contain three homologous proteins: phytohaemagglutinin E, phytohaemagglutinin L and the lectin-like protein alpha-amylase inhibitor (alpha AI).
T3 275-638 Sentence denotes Whereas the active site of lectins has been studied in great detail, there is no information on the active site of the related protein alpha AI, which exerts its biological activity by making a 1:1 complex with alpha-amylase. alpha-amylase inhibitor is synthesized as a 30 kDa precursor glycoprotein that needs to be processed at Asn77 to form an active molecule.
T4 639-836 Sentence denotes Comparison of the amino acid sequence of the bean alpha AI with that of the bacterial amylase inhibitor, tendamistat, suggested that a region around Trp188 might be involved in the inhibitory site.
T5 837-986 Sentence denotes When a three-dimensional model of the bean alpha AI was constructed based on its homology to the legume lectins, this Trp region was alongside Asn77.
T6 987-1127 Sentence denotes To test this site hypothesis, mutants of alpha AI were created by site-directed mutagenesis of the cDNA and expressed in transgenic tobacco.
T7 1128-1230 Sentence denotes The mutant proteins R74N and WSY188-190GNV, as well as the double mutant, were inactive as inhibitors.
T8 1231-1483 Sentence denotes These findings suggest that the active site of alpha AI consists of W188, R74 and Y190, in analogy to the Trp-Arg-Tyr motif of tendamistat, and that the processing of the polypeptide at Asn77 may be necessary to bring these residues in close proximity.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 1473-1482 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximity

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 156-164 FMAID:67257 denotes proteins
_T2 156-164 FMAID:165447 denotes proteins
_T3 231-238 FMAID:165447 denotes protein
_T4 231-238 FMAID:67257 denotes protein
_T5 402-409 FMAID:165447 denotes protein
_T6 402-409 FMAID:67257 denotes protein
_T7 562-574 FMAID:167256 denotes glycoprotein
_T8 562-574 FMAID:62925 denotes glycoprotein
_T9 589-601 FMAID:181526 denotes be processed
_T10 589-601 FMAID:75433 denotes be processed
_T11 657-667 FMAID:82739 denotes amino acid
_T12 657-667 FMAID:196728 denotes amino acid
_T13 990-994 FMAID:178661 denotes test
_T14 1139-1147 FMAID:165447 denotes proteins
_T15 1139-1147 FMAID:67257 denotes proteins
_T16 1473-1482 FMAID:171415 denotes proximity
_T17 1473-1482 FMAID:30326 denotes proximity

uniprot-human

Id Subject Object Predicate Lexical cue
T1 394-415 http://www.uniprot.org/uniprot/Q9UBT7 denotes related protein alpha
T2 394-415 http://www.uniprot.org/uniprot/Q53T67 denotes related protein alpha

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 85-88 http://www.uniprot.org/uniprot/P62340 denotes Trp
T2 955-958 http://www.uniprot.org/uniprot/P62340 denotes Trp
T3 1337-1340 http://www.uniprot.org/uniprot/P62340 denotes Trp
T4 95-98 http://www.uniprot.org/uniprot/P11344 denotes Tyr
T5 1345-1348 http://www.uniprot.org/uniprot/P11344 denotes Tyr
T6 330-335 http://www.uniprot.org/uniprot/Q91ZZ5 denotes great

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 394-401 http://purl.bioontology.org/ontology/NCBITAXON/353209 denotes related
T2 657-676 http://purl.bioontology.org/ontology/STY/T087 denotes amino acid sequence
T3 1237-1245 http://purl.bioontology.org/ontology/STY/T033 denotes findings

GO-BP

Id Subject Object Predicate Lexical cue
T1 40-63 http://purl.obolibrary.org/obo/GO_0015066 denotes alpha-amylase inhibitor
T2 239-262 http://purl.obolibrary.org/obo/GO_0015066 denotes alpha-amylase inhibitor
T3 245-269 http://purl.obolibrary.org/obo/GO_0015066 denotes amylase inhibitor (alpha

GO-CC

Id Subject Object Predicate Lexical cue
T1 99-104 http://purl.obolibrary.org/obo/GO_0030315 denotes triad

GlycoBiology-MAT

Id Subject Object Predicate Lexical cue
T1 1473-1482 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximity

Lectin-Jamboree

Id Subject Object Predicate Lexical cue
T1 219-225 lectin denotes lectin
T2 302-309 lectin denotes lectins
T3 941-948 lectin denotes lectins

Lectin-Jamboree-Sentence

Id Subject Object Predicate Lexical cue
T1 0-105 Sentence denotes Location of the active site of the bean alpha-amylase inhibitor and involvement of a Trp, Arg, Tyr triad.
T2 106-274 Sentence denotes Seeds of the common bean contain three homologous proteins: phytohaemagglutinin E, phytohaemagglutinin L and the lectin-like protein alpha-amylase inhibitor (alpha AI).
T3 275-638 Sentence denotes Whereas the active site of lectins has been studied in great detail, there is no information on the active site of the related protein alpha AI, which exerts its biological activity by making a 1:1 complex with alpha-amylase. alpha-amylase inhibitor is synthesized as a 30 kDa precursor glycoprotein that needs to be processed at Asn77 to form an active molecule.
T4 639-836 Sentence denotes Comparison of the amino acid sequence of the bean alpha AI with that of the bacterial amylase inhibitor, tendamistat, suggested that a region around Trp188 might be involved in the inhibitory site.
T5 837-986 Sentence denotes When a three-dimensional model of the bean alpha AI was constructed based on its homology to the legume lectins, this Trp region was alongside Asn77.
T6 987-1127 Sentence denotes To test this site hypothesis, mutants of alpha AI were created by site-directed mutagenesis of the cDNA and expressed in transgenic tobacco.
T7 1128-1230 Sentence denotes The mutant proteins R74N and WSY188-190GNV, as well as the double mutant, were inactive as inhibitors.
T8 1231-1483 Sentence denotes These findings suggest that the active site of alpha AI consists of W188, R74 and Y190, in analogy to the Trp-Arg-Tyr motif of tendamistat, and that the processing of the polypeptide at Asn77 may be necessary to bring these residues in close proximity.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1119-1126 OrganismTaxon denotes tobacco 4097

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 1473-1482 Body_part denotes proximity http://purl.obolibrary.org/obo/MAT_0000491