PubMed:7689721 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/7689721","sourcedb":"PubMed","sourceid":"7689721","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/7689721","text":"Identification and characterization of multiple mdm-2 proteins and mdm-2-p53 protein complexes.\nA protein product of the mdm-2 oncogene (p90) has been recently shown to associate with the protein encoded by the tumor-suppressor gene p53. The mdm-2 gene was originally identified as a gene amplified in a spontaneously transformed Balb/c 3T3 cell line (3T3DM). This report describes the characterization of mdm-2 gene products and their interactions with the p53 protein. Polyclonal and monoclonal antibodies were generated against murine and human mdm-2 protein. These antibodies detected the mdm-2 p90 protein and at least four additional polypeptides (p85, p76, p74, p58-p57) in cultured cells. These additional proteins may arise from different spliced mRNA forms of the mdm-2 gene or post-translational modifications of the mdm-2 protein. The monoclonal antibodies distinguished at least three sets of mdm-2 proteins with distinct combinations of epitopes (p90 and p85; p76 and p74; p58-57). One or two of these proteins forms a complex with the p53 protein (p90, p58). These mdm-2 proteins were found to be overexpressed in 3T3DM cells and a subset of these proteins were complexed with p53. In 3T3DM cells, p90, like p53, had a short half-life of approximately 20 min and was localized to the cell nucleus. In resting cells stimulated with serum p90 levels and p90/p53 complex levels increased in the late G1 phase of the cell cycle. The p90 mdm-2 protein could regulate p53 activity in the late G1 phase of the cell cycle.","tracks":[{"project":"LocText","denotations":[{"id":"T1","span":{"begin":48,"end":53},"obj":"uniprot:P23804"},{"id":"T2","span":{"begin":48,"end":53},"obj":"uniprot:Q00987"},{"id":"T3","span":{"begin":67,"end":72},"obj":"uniprot:P23804"},{"id":"T4","span":{"begin":67,"end":72},"obj":"uniprot:Q00987"},{"id":"T5","span":{"begin":73,"end":76},"obj":"uniprot:P02340"},{"id":"T6","span":{"begin":73,"end":76},"obj":"uniprot:P04637"},{"id":"T7","span":{"begin":121,"end":126},"obj":"uniprot:P23804"},{"id":"T8","span":{"begin":121,"end":126},"obj":"uniprot:Q00987"},{"id":"T9","span":{"begin":137,"end":140},"obj":"uniprot:Q8TCG1"},{"id":"T10","span":{"begin":137,"end":140},"obj":"uniprot:Q61586"},{"id":"T11","span":{"begin":233,"end":236},"obj":"uniprot:P02340"},{"id":"T12","span":{"begin":233,"end":236},"obj":"uniprot:P04637"},{"id":"T13","span":{"begin":242,"end":247},"obj":"uniprot:P23804"},{"id":"T14","span":{"begin":242,"end":247},"obj":"uniprot:Q00987"},{"id":"T15","span":{"begin":406,"end":411},"obj":"uniprot:P23804"},{"id":"T16","span":{"begin":406,"end":411},"obj":"uniprot:Q00987"},{"id":"T17","span":{"begin":458,"end":461},"obj":"uniprot:P02340"},{"id":"T18","span":{"begin":458,"end":461},"obj":"uniprot:P04637"},{"id":"T19","span":{"begin":531,"end":537},"obj":"taxonomy:10090"},{"id":"T20","span":{"begin":542,"end":547},"obj":"taxonomy:9606"},{"id":"T21","span":{"begin":548,"end":553},"obj":"uniprot:P23804"},{"id":"T22","span":{"begin":548,"end":553},"obj":"uniprot:Q00987"},{"id":"T23","span":{"begin":593,"end":598},"obj":"uniprot:P23804"},{"id":"T24","span":{"begin":593,"end":598},"obj":"uniprot:Q00987"},{"id":"T25","span":{"begin":599,"end":602},"obj":"uniprot:Q8TCG1"},{"id":"T26","span":{"begin":599,"end":602},"obj":"uniprot:Q61586"},{"id":"T27","span":{"begin":654,"end":657},"obj":"uniprot:uniprot:"},{"id":"T28","span":{"begin":659,"end":662},"obj":"uniprot:Q3TCN2"},{"id":"T29","span":{"begin":659,"end":662},"obj":"uniprot:Q8NHP8"},{"id":"T30","span":{"begin":664,"end":667},"obj":"uniprot:uniprot:"},{"id":"T31","span":{"begin":669,"end":672},"obj":"uniprot:uniprot:"},{"id":"T32","span":{"begin":673,"end":676},"obj":"uniprot:uniprot:"},{"id":"T33","span":{"begin":774,"end":779},"obj":"uniprot:P23804"},{"id":"T34","span":{"begin":774,"end":779},"obj":"uniprot:Q00987"},{"id":"T35","span":{"begin":828,"end":833},"obj":"uniprot:P23804"},{"id":"T36","span":{"begin":828,"end":833},"obj":"uniprot:Q00987"},{"id":"T37","span":{"begin":906,"end":911},"obj":"uniprot:P23804"},{"id":"T38","span":{"begin":906,"end":911},"obj":"uniprot:Q00987"},{"id":"T39","span":{"begin":961,"end":964},"obj":"uniprot:Q8TCG1"},{"id":"T40","span":{"begin":961,"end":964},"obj":"uniprot:Q61586"},{"id":"T41","span":{"begin":969,"end":972},"obj":"uniprot:uniprot:"},{"id":"T42","span":{"begin":974,"end":977},"obj":"uniprot:Q3TCN2"},{"id":"T43","span":{"begin":974,"end":977},"obj":"uniprot:Q8NHP8"},{"id":"T44","span":{"begin":982,"end":985},"obj":"uniprot:uniprot:"},{"id":"T45","span":{"begin":987,"end":993},"obj":"uniprot:uniprot:"},{"id":"T46","span":{"begin":1050,"end":1053},"obj":"uniprot:P02340"},{"id":"T47","span":{"begin":1050,"end":1053},"obj":"uniprot:P04637"},{"id":"T48","span":{"begin":1063,"end":1066},"obj":"uniprot:Q8TCG1"},{"id":"T49","span":{"begin":1063,"end":1066},"obj":"uniprot:Q61586"},{"id":"T50","span":{"begin":1068,"end":1071},"obj":"uniprot:uniprot:"},{"id":"T51","span":{"begin":1080,"end":1085},"obj":"uniprot:P23804"},{"id":"T52","span":{"begin":1080,"end":1085},"obj":"uniprot:Q00987"},{"id":"T53","span":{"begin":1192,"end":1195},"obj":"uniprot:P02340"},{"id":"T54","span":{"begin":1192,"end":1195},"obj":"uniprot:P04637"},{"id":"T55","span":{"begin":1213,"end":1216},"obj":"uniprot:Q8TCG1"},{"id":"T56","span":{"begin":1213,"end":1216},"obj":"uniprot:Q61586"},{"id":"T57","span":{"begin":1223,"end":1226},"obj":"uniprot:P02340"},{"id":"T58","span":{"begin":1223,"end":1226},"obj":"uniprot:P04637"},{"id":"T59","span":{"begin":1304,"end":1311},"obj":"go:GO:0005634"},{"id":"T60","span":{"begin":1352,"end":1355},"obj":"uniprot:Q8TCG1"},{"id":"T61","span":{"begin":1352,"end":1355},"obj":"uniprot:Q61586"},{"id":"T62","span":{"begin":1367,"end":1370},"obj":"uniprot:Q8TCG1"},{"id":"T63","span":{"begin":1367,"end":1370},"obj":"uniprot:Q61586"},{"id":"T64","span":{"begin":1371,"end":1374},"obj":"uniprot:P02340"},{"id":"T65","span":{"begin":1371,"end":1374},"obj":"uniprot:P04637"},{"id":"T66","span":{"begin":1444,"end":1447},"obj":"uniprot:Q8TCG1"},{"id":"T67","span":{"begin":1444,"end":1447},"obj":"uniprot:Q61586"},{"id":"T68","span":{"begin":1448,"end":1453},"obj":"uniprot:P23804"},{"id":"T69","span":{"begin":1448,"end":1453},"obj":"uniprot:Q00987"},{"id":"T70","span":{"begin":1477,"end":1480},"obj":"uniprot:P02340"},{"id":"T71","span":{"begin":1477,"end":1480},"obj":"uniprot:P04637"}],"relations":[{"id":"R1","pred":"localizeTo","subj":"T55","obj":"T59"},{"id":"R2","pred":"localizeTo","subj":"T57","obj":"T59"}],"namespaces":[{"prefix":"uniprot","uri":"http://identifiers.org/uniprot/"},{"prefix":"taxonomy","uri":"http://identifiers.org/taxonomy/"},{"prefix":"go","uri":"http://identifiers.org/go/"}],"attributes":[{"subj":"T1","pred":"source","obj":"LocText"},{"subj":"T2","pred":"source","obj":"LocText"},{"subj":"T3","pred":"source","obj":"LocText"},{"subj":"T4","pred":"source","obj":"LocText"},{"subj":"T5","pred":"source","obj":"LocText"},{"subj":"T6","pred":"source","obj":"LocText"},{"subj":"T7","pred":"source","obj":"LocText"},{"subj":"T8","pred":"source","obj":"LocText"},{"subj":"T9","pred":"source","obj":"LocText"},{"subj":"T10","pred":"source","obj":"LocText"},{"subj":"T11","pred":"source","obj":"LocText"},{"subj":"T12","pred":"source","obj":"LocText"},{"subj":"T13","pred":"source","obj":"LocText"},{"subj":"T14","pred":"source","obj":"LocText"},{"subj":"T15","pred":"source","obj":"LocText"},{"subj":"T16","pred":"source","obj":"LocText"},{"subj":"T17","pred":"source","obj":"LocText"},{"subj":"T18","pred":"source","obj":"LocText"},{"subj":"T19","pred":"source","obj":"LocText"},{"subj":"T20","pred":"source","obj":"LocText"},{"subj":"T21","pred":"source","obj":"LocText"},{"subj":"T22","pred":"source","obj":"LocText"},{"subj":"T23","pred":"source","obj":"LocText"},{"subj":"T24","pred":"source","obj":"LocText"},{"subj":"T25","pred":"source","obj":"LocText"},{"subj":"T26","pred":"source","obj":"LocText"},{"subj":"T27","pred":"source","obj":"LocText"},{"subj":"T28","pred":"source","obj":"LocText"},{"subj":"T29","pred":"source","obj":"LocText"},{"subj":"T30","pred":"source","obj":"LocText"},{"subj":"T31","pred":"source","obj":"LocText"},{"subj":"T32","pred":"source","obj":"LocText"},{"subj":"T33","pred":"source","obj":"LocText"},{"subj":"T34","pred":"source","obj":"LocText"},{"subj":"T35","pred":"source","obj":"LocText"},{"subj":"T36","pred":"source","obj":"LocText"},{"subj":"T37","pred":"source","obj":"LocText"},{"subj":"T38","pred":"source","obj":"LocText"},{"subj":"T39","pred":"source","obj":"LocText"},{"subj":"T40","pred":"source","obj":"LocText"},{"subj":"T41","pred":"source","obj":"LocText"},{"subj":"T42","pred":"source","obj":"LocText"},{"subj":"T43","pred":"source","obj":"LocText"},{"subj":"T44","pred":"source","obj":"LocText"},{"subj":"T45","pred":"source","obj":"LocText"},{"subj":"T46","pred":"source","obj":"LocText"},{"subj":"T47","pred":"source","obj":"LocText"},{"subj":"T48","pred":"source","obj":"LocText"},{"subj":"T49","pred":"source","obj":"LocText"},{"subj":"T50","pred":"source","obj":"LocText"},{"subj":"T51","pred":"source","obj":"LocText"},{"subj":"T52","pred":"source","obj":"LocText"},{"subj":"T53","pred":"source","obj":"LocText"},{"subj":"T54","pred":"source","obj":"LocText"},{"subj":"T55","pred":"source","obj":"LocText"},{"subj":"T56","pred":"source","obj":"LocText"},{"subj":"T57","pred":"source","obj":"LocText"},{"subj":"T58","pred":"source","obj":"LocText"},{"subj":"T59","pred":"source","obj":"LocText"},{"subj":"T60","pred":"source","obj":"LocText"},{"subj":"T61","pred":"source","obj":"LocText"},{"subj":"T62","pred":"source","obj":"LocText"},{"subj":"T63","pred":"source","obj":"LocText"},{"subj":"T64","pred":"source","obj":"LocText"},{"subj":"T65","pred":"source","obj":"LocText"},{"subj":"T66","pred":"source","obj":"LocText"},{"subj":"T67","pred":"source","obj":"LocText"},{"subj":"T68","pred":"source","obj":"LocText"},{"subj":"T69","pred":"source","obj":"LocText"},{"subj":"T70","pred":"source","obj":"LocText"},{"subj":"T71","pred":"source","obj":"LocText"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LocText","color":"#93ecc4","default":true}]}]}}