PubMed:7685215 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/7685215","sourcedb":"PubMed","sourceid":"7685215","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/7685215","text":"The bZIP domains of Fos and Jun mediate a physical association with the TATA box-binding protein.\nFos and Jun oncoproteins form a complex that regulates transcription from promoters containing AP-1 binding sites. These two proteins, like other transcriptional activators, are likely to stimulate transcription through direct and/or indirect interactions with members of the basal transcriptional machinery. The ability of c-Fos and c-Jun proteins to interact directly with the TATA box-binding protein (TBP), the general transcription factor required for initiating the assembly of transcription complexes, was investigated. Using co-immunoprecipitation and protein-protein association assays, we show that both c-Fos and c-Jun bind specifically and stably to TBP. Mutational analysis demonstrates that both the basic region and leucine zipper domains of c-Fos and c-Jun are necessary and sufficient for stable association with TBP. A 51-residue region from the conserved C-terminal region of TBP, previously shown to be the binding site for the viral activator protein E1A, interacts with c-Fos and c-Jun proteins. We propose that c-Fos and c-Jun proteins function as transcriptional activators, in part by recruiting TBP to form complexes to initiate RNA synthesis.","tracks":[{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":20,"end":23},"obj":"protein"},{"id":"T2","span":{"begin":28,"end":31},"obj":"protein"},{"id":"T3","span":{"begin":72,"end":96},"obj":"protein"},{"id":"T4","span":{"begin":98,"end":101},"obj":"protein"},{"id":"T5","span":{"begin":106,"end":109},"obj":"protein"},{"id":"T6","span":{"begin":422,"end":427},"obj":"protein"},{"id":"T7","span":{"begin":432,"end":437},"obj":"protein"},{"id":"T8","span":{"begin":477,"end":501},"obj":"protein"},{"id":"T9","span":{"begin":503,"end":506},"obj":"protein"},{"id":"T10","span":{"begin":712,"end":717},"obj":"protein"},{"id":"T11","span":{"begin":722,"end":727},"obj":"protein"},{"id":"T12","span":{"begin":760,"end":763},"obj":"protein"},{"id":"T13","span":{"begin":855,"end":860},"obj":"protein"},{"id":"T14","span":{"begin":865,"end":870},"obj":"protein"},{"id":"T15","span":{"begin":928,"end":931},"obj":"protein"},{"id":"T16","span":{"begin":993,"end":996},"obj":"protein"},{"id":"T17","span":{"begin":1070,"end":1073},"obj":"protein"},{"id":"T18","span":{"begin":1090,"end":1095},"obj":"protein"},{"id":"T19","span":{"begin":1100,"end":1105},"obj":"protein"},{"id":"T20","span":{"begin":1132,"end":1137},"obj":"protein"},{"id":"T21","span":{"begin":1142,"end":1147},"obj":"protein"},{"id":"T22","span":{"begin":1219,"end":1222},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"AIMed"},{"subj":"T2","pred":"source","obj":"AIMed"},{"subj":"T3","pred":"source","obj":"AIMed"},{"subj":"T4","pred":"source","obj":"AIMed"},{"subj":"T5","pred":"source","obj":"AIMed"},{"subj":"T6","pred":"source","obj":"AIMed"},{"subj":"T7","pred":"source","obj":"AIMed"},{"subj":"T8","pred":"source","obj":"AIMed"},{"subj":"T9","pred":"source","obj":"AIMed"},{"subj":"T10","pred":"source","obj":"AIMed"},{"subj":"T11","pred":"source","obj":"AIMed"},{"subj":"T12","pred":"source","obj":"AIMed"},{"subj":"T13","pred":"source","obj":"AIMed"},{"subj":"T14","pred":"source","obj":"AIMed"},{"subj":"T15","pred":"source","obj":"AIMed"},{"subj":"T16","pred":"source","obj":"AIMed"},{"subj":"T17","pred":"source","obj":"AIMed"},{"subj":"T18","pred":"source","obj":"AIMed"},{"subj":"T19","pred":"source","obj":"AIMed"},{"subj":"T20","pred":"source","obj":"AIMed"},{"subj":"T21","pred":"source","obj":"AIMed"},{"subj":"T22","pred":"source","obj":"AIMed"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AIMed","color":"#eca493","default":true}]}]}}