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PubMed:7685022 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-47 Sentence denotes RNA-directed RNA polymerase from tomato leaves.
T2 48-50 Sentence denotes I.
T3 51-88 Sentence denotes Purification and physical properties.
T4 89-208 Sentence denotes An RNA-directed RNA polymerase (RdRP, EC 2.7.7.48) from tomato leaf tissue was purified to electrophoretic homogeneity.
T5 209-378 Sentence denotes A terminal transferase activity that was found to cofractionate with RdRP from DEAE-Sepharose and DNA-cellulose columns was removed by chromatography on a Mono Q column.
T6 379-525 Sentence denotes The highly purified RdRP exhibits a specific activity of 500 nmol x mg-1 x 30 min-1, which corresponds to a 100,000-fold enrichment of the enzyme.
T7 526-603 Sentence denotes In buffer containing 50% glycerol, its activity decreased by about 15%/month.
T8 604-805 Sentence denotes RdRP activity coincided with the silver staining intensity of a single 128-kDa polypeptide when the fractions eluted from the Mono Q column were analyzed by electrophoresis in a SDS-polyacrylamide gel.
T9 806-917 Sentence denotes Its molecular mass and its sedimentation coefficient of 6.6 S indicate that RdRP is a nearly globular molecule.
T10 918-998 Sentence denotes The catalytic activity of RdRP is resistant to alpha-amanitin and actinomycin D.
T11 999-1185 Sentence denotes In tomato leaves systemically infected with potato spindle tuber viroid, the activity of RdRP was found to be increased about 3-fold compared with RdRP isolated from healthy leaf tissue.
T1 0-47 Sentence denotes RNA-directed RNA polymerase from tomato leaves.
T2 48-50 Sentence denotes I.
T3 51-88 Sentence denotes Purification and physical properties.
T4 89-208 Sentence denotes An RNA-directed RNA polymerase (RdRP, EC 2.7.7.48) from tomato leaf tissue was purified to electrophoretic homogeneity.
T5 209-378 Sentence denotes A terminal transferase activity that was found to cofractionate with RdRP from DEAE-Sepharose and DNA-cellulose columns was removed by chromatography on a Mono Q column.
T6 379-525 Sentence denotes The highly purified RdRP exhibits a specific activity of 500 nmol x mg-1 x 30 min-1, which corresponds to a 100,000-fold enrichment of the enzyme.
T7 526-603 Sentence denotes In buffer containing 50% glycerol, its activity decreased by about 15%/month.
T8 604-805 Sentence denotes RdRP activity coincided with the silver staining intensity of a single 128-kDa polypeptide when the fractions eluted from the Mono Q column were analyzed by electrophoresis in a SDS-polyacrylamide gel.
T9 806-917 Sentence denotes Its molecular mass and its sedimentation coefficient of 6.6 S indicate that RdRP is a nearly globular molecule.
T10 918-998 Sentence denotes The catalytic activity of RdRP is resistant to alpha-amanitin and actinomycin D.
T11 999-1185 Sentence denotes In tomato leaves systemically infected with potato spindle tuber viroid, the activity of RdRP was found to be increased about 3-fold compared with RdRP isolated from healthy leaf tissue.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 1058-1063 http://purl.obolibrary.org/obo/MAT_0000358 denotes tuber

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 637-652 Disease denotes silver staining http://purl.obolibrary.org/obo/MONDO_0018952

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 1058-1063 Body_part denotes tuber http://purl.obolibrary.org/obo/MAT_0000358

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1043-1070 OrganismTaxon denotes potato spindle tuber viroid 12892

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 157-163 Body_part denotes tissue http://purl.obolibrary.org/obo/UBERON_0000479
T2 1058-1063 Body_part denotes tuber http://purl.obolibrary.org/obo/UBERON_0005346|http://purl.obolibrary.org/obo/UBERON_0005813
T4 1178-1184 Body_part denotes tissue http://purl.obolibrary.org/obo/UBERON_0000479