PubMed:7673116 JSON TXT

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sentences

Id	Subject	Object	Predicate	Lexical cue
T1	0-136	Sentence	denotes	A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.
T2	137-279	Sentence	denotes	Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription.
T3	280-462	Sentence	denotes	Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined.
T4	463-586	Sentence	denotes	Recent reports suggest that phosphorylation of Pit-1 may not be required for hormonal regulation of the prolactin promoter.
T5	587-736	Sentence	denotes	Analysis of the contribution of individual Pit-1 binding sites has been complicated due to the fact that some of the elements appear to be redundant.
T6	737-993	Sentence	denotes	To better understand the role of Pit-1 sites in mediating hormonal regulation of the prolactin gene, we have performed enhancer tests using the three most proximal Pit-1 binding sites of the rat prolactin gene which are designated the 1P, 2P, and 3P sites.
T7	994-1181	Sentence	denotes	The results demonstrate that multimers of the 3P Pit-1 binding site are much more responsive to several hormonal and intracellular signaling pathways than multimers of the 1P or 2P sites.
T8	1182-1278	Sentence	denotes	The 3P DNA element was found to contain a consensus binding site for the Ets family of proteins.
T9	1279-1442	Sentence	denotes	Mutation of the Ets binding site greatly decreased the ability of epidermal growth factor, phorbol esters, Ras, or the Raf kinase to induce reporter gene activity.
T10	1443-1513	Sentence	denotes	Mutation of the Ets site had little effect on basal enhancer activity.
T11	1514-1640	Sentence	denotes	In contrast, mutation of the consensus Pit-1 binding site in the 3P element essentially abolished all basal enhancer activity.
T12	1641-1754	Sentence	denotes	Overexpression of Ets-1 in GH3 pituitary cells enhanced both basal and Ras induced activity from the 3P enhancer.
T13	1755-2028	Sentence	denotes	These data describe a composite element in the prolactin gene containing binding sites for two different factors and the studies suggest a mechanism by which Ets proteins and Pit-1 functionally cooperate to permit transcriptional regulation by different signaling pathways.
T1	0-136	Sentence	denotes	A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.
T2	137-279	Sentence	denotes	Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription.
T3	280-462	Sentence	denotes	Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined.
T4	463-586	Sentence	denotes	Recent reports suggest that phosphorylation of Pit-1 may not be required for hormonal regulation of the prolactin promoter.
T5	587-736	Sentence	denotes	Analysis of the contribution of individual Pit-1 binding sites has been complicated due to the fact that some of the elements appear to be redundant.
T6	737-993	Sentence	denotes	To better understand the role of Pit-1 sites in mediating hormonal regulation of the prolactin gene, we have performed enhancer tests using the three most proximal Pit-1 binding sites of the rat prolactin gene which are designated the 1P, 2P, and 3P sites.
T7	994-1181	Sentence	denotes	The results demonstrate that multimers of the 3P Pit-1 binding site are much more responsive to several hormonal and intracellular signaling pathways than multimers of the 1P or 2P sites.
T8	1182-1278	Sentence	denotes	The 3P DNA element was found to contain a consensus binding site for the Ets family of proteins.
T9	1279-1442	Sentence	denotes	Mutation of the Ets binding site greatly decreased the ability of epidermal growth factor, phorbol esters, Ras, or the Raf kinase to induce reporter gene activity.
T10	1443-1513	Sentence	denotes	Mutation of the Ets site had little effect on basal enhancer activity.
T11	1514-1640	Sentence	denotes	In contrast, mutation of the consensus Pit-1 binding site in the 3P element essentially abolished all basal enhancer activity.
T12	1641-1754	Sentence	denotes	Overexpression of Ets-1 in GH3 pituitary cells enhanced both basal and Ras induced activity from the 3P enhancer.
T13	1755-2028	Sentence	denotes	These data describe a composite element in the prolactin gene containing binding sites for two different factors and the studies suggest a mechanism by which Ets proteins and Pit-1 functionally cooperate to permit transcriptional regulation by different signaling pathways.

Glycosmos6-MAT

Id	Subject	Object	Predicate	Lexical cue
T1	892-900	http://purl.obolibrary.org/obo/MAT_0000491	denotes	proximal
T2	1672-1681	http://purl.obolibrary.org/obo/MAT_0000077	denotes	pituitary

Anatomy-MAT

Id	Subject	Object	Predicate	Lexical cue	mat_id
T1	892-900	Body_part	denotes	proximal	http://purl.obolibrary.org/obo/MAT_0000491
T2	1672-1681	Body_part	denotes	pituitary	http://purl.obolibrary.org/obo/MAT_0000077

mondo_disease

Id	Subject	Object	Predicate	Lexical cue	mondo_id
T1	1672-1681	Disease	denotes	pituitary	http://purl.obolibrary.org/obo/MONDO_0021156

NCBITAXON

Id	Subject	Object	Predicate	Lexical cue	db_id
T1	928-931	OrganismTaxon	denotes	rat	10114\|10116

Anatomy-UBERON

Id	Subject	Object	Predicate	Lexical cue	uberon_id
T1	159-165	Body_part	denotes	tissue	http://purl.obolibrary.org/obo/UBERON_0000479
T2	1111-1124	Body_part	denotes	intracellular	http://purl.obolibrary.org/obo/GO_0005622
T3	1345-1361	Body_part	denotes	epidermal growth	http://purl.obolibrary.org/obo/UBERON_0000021
T4	1672-1681	Body_part	denotes	pituitary	http://purl.obolibrary.org/obo/UBERON_0000007

CL-cell

Id	Subject	Object	Predicate	Lexical cue	cl_id
T1	221-226	Cell	denotes	basal	http://purl.obolibrary.org/obo/CL:0002324
T2	1489-1494	Cell	denotes	basal	http://purl.obolibrary.org/obo/CL:0002324
T3	1616-1621	Cell	denotes	basal	http://purl.obolibrary.org/obo/CL:0002324
T4	1702-1707	Cell	denotes	basal	http://purl.obolibrary.org/obo/CL:0002324