PubMed:7598686 JSONTXT

{"project":"PennBioIE","denotations":[{"id":"T1","span":{"begin":48,"end":57},"obj":"protein"},{"id":"T2","span":{"begin":67,"end":80},"obj":"protein"},{"id":"T3","span":{"begin":231,"end":258},"obj":"protein"},{"id":"T4","span":{"begin":260,"end":269},"obj":"protein"},{"id":"T5","span":{"begin":314,"end":323},"obj":"protein"},{"id":"T6","span":{"begin":332,"end":345},"obj":"protein"},{"id":"T7","span":{"begin":458,"end":467},"obj":"protein"},{"id":"T8","span":{"begin":542,"end":555},"obj":"protein"},{"id":"T9","span":{"begin":624,"end":637},"obj":"protein"},{"id":"T10","span":{"begin":790,"end":803},"obj":"protein"},{"id":"T11","span":{"begin":985,"end":994},"obj":"protein"}],"text":"Pentoxifylline augments but does not antagonize TNF alpha-mediated neuroblastoma cell differentiation: modulation of calcium mobilization but not cAMP.\nWe described here that pentoxifylline (PTX), which is well known to counteract tumor necrosis factor alpha (TNF alpha)-mediated inflammatory responses, augmented TNF alpha-induced neuroblastoma cell differentiation in conjunction with growth inhibition and cell-cycle arrest in G1 phase. PTX also enhanced TNF alpha-induced down-regulation of acetylcholine-mediated [Ca2+]i mobilization in neuroblastoma cells. Furthermore, we found that addition of cAMP failed to induce neuroblastoma cell differentiation, whereas blockade of [Ca2+]i mobilization by 8-(N,N-diethyl-amino)octyl-3,4,5-trimethoxybenzoate HCl (TMB-8, 10 microM) did induce neuroblastoma cell differentiation. Taken together, these results indicated that PTX possessed a novel signal transduction, down-regulation of [Ca2+]i mobilization, to augment but not counteract TNF alpha-mediated functions."}