| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-103 |
Sentence |
denotes |
The mitogenic effects of the B beta chain of fibrinogen are mediated through cell surface calreticulin. |
| T2 |
104-339 |
Sentence |
denotes |
We have previously shown that soluble partially degraded fibrin(ogen) remains in solution after fibrin clot formation and is a potent fibroblast mitogen (Gray, A.J., Bishop, J.E., Reeves J.T., Mecham, R.P., and Laurent, G.J. (1995) Am. |
| T3 |
340-342 |
Sentence |
denotes |
J. |
| T4 |
343-352 |
Sentence |
denotes |
Cell Mol. |
| T5 |
353-358 |
Sentence |
denotes |
Biol. |
| T6 |
359-372 |
Sentence |
denotes |
12, 684-690). |
| T7 |
373-505 |
Sentence |
denotes |
Mitogenic sites within the fibrin(ogen) molecule are located on the A alpha and B beta chains of the protein (Gray, A.J., Bishop, J. |
| T8 |
506-551 |
Sentence |
denotes |
E., Reeves, J.T., and Laurent, G.J. (1993) J. |
| T9 |
552-561 |
Sentence |
denotes |
Cell Sci. |
| T10 |
562-576 |
Sentence |
denotes |
104, 409-413). |
| T11 |
577-661 |
Sentence |
denotes |
However, receptor pathways through which mitogenic effects are mediated are unknown. |
| T12 |
662-816 |
Sentence |
denotes |
The present study sought to determine the nature of fibrin(ogen) receptors expressed on human fibroblasts which interact with the fibrinogen B beta chain. |
| T13 |
817-946 |
Sentence |
denotes |
Receptor complexes were isolated from 125I-surface-labeled fibroblasts and purified on a fibrinogen B beta chain affinity column. |
| T14 |
947-1130 |
Sentence |
denotes |
Subsequent high performance liquid chromatography and SDS-polyacrylamide gel electrophoresis analysis indicated fibrinogen B beta chain bound specifically to a 60-kDa surface protein. |
| T15 |
1131-1233 |
Sentence |
denotes |
Sequence analysis of the amino terminus of this protein indicated 100% homology to human calreticulin. |
| T16 |
1234-1406 |
Sentence |
denotes |
Immunoprecipitation experiments employing a polyclonal anti-calreticulin antibody provided further evidence that the 60-kDa protein isolated in this study was calreticulin. |
| T17 |
1407-1555 |
Sentence |
denotes |
Further, polyclonal antibodies to human calreticulin significantly inhibited the mitogenic activity of fibrinogen B beta chain on human fibroblasts. |
| T18 |
1556-1688 |
Sentence |
denotes |
The present study has shown that cell surface calreticulin binds to the B beta chain of fibrinogen mediating its mitogenic activity. |
| T1 |
0-103 |
Sentence |
denotes |
The mitogenic effects of the B beta chain of fibrinogen are mediated through cell surface calreticulin. |
| T2 |
104-339 |
Sentence |
denotes |
We have previously shown that soluble partially degraded fibrin(ogen) remains in solution after fibrin clot formation and is a potent fibroblast mitogen (Gray, A.J., Bishop, J.E., Reeves J.T., Mecham, R.P., and Laurent, G.J. (1995) Am. |
| T3 |
340-342 |
Sentence |
denotes |
J. |
| T4 |
343-352 |
Sentence |
denotes |
Cell Mol. |
| T5 |
353-358 |
Sentence |
denotes |
Biol. |
| T6 |
359-372 |
Sentence |
denotes |
12, 684-690). |
| T7 |
373-505 |
Sentence |
denotes |
Mitogenic sites within the fibrin(ogen) molecule are located on the A alpha and B beta chains of the protein (Gray, A.J., Bishop, J. |
| T8 |
506-551 |
Sentence |
denotes |
E., Reeves, J.T., and Laurent, G.J. (1993) J. |
| T9 |
552-561 |
Sentence |
denotes |
Cell Sci. |
| T10 |
562-576 |
Sentence |
denotes |
104, 409-413). |
| T11 |
577-661 |
Sentence |
denotes |
However, receptor pathways through which mitogenic effects are mediated are unknown. |
| T12 |
662-816 |
Sentence |
denotes |
The present study sought to determine the nature of fibrin(ogen) receptors expressed on human fibroblasts which interact with the fibrinogen B beta chain. |
| T13 |
817-946 |
Sentence |
denotes |
Receptor complexes were isolated from 125I-surface-labeled fibroblasts and purified on a fibrinogen B beta chain affinity column. |
| T14 |
947-1130 |
Sentence |
denotes |
Subsequent high performance liquid chromatography and SDS-polyacrylamide gel electrophoresis analysis indicated fibrinogen B beta chain bound specifically to a 60-kDa surface protein. |
| T15 |
1131-1233 |
Sentence |
denotes |
Sequence analysis of the amino terminus of this protein indicated 100% homology to human calreticulin. |
| T16 |
1234-1406 |
Sentence |
denotes |
Immunoprecipitation experiments employing a polyclonal anti-calreticulin antibody provided further evidence that the 60-kDa protein isolated in this study was calreticulin. |
| T17 |
1407-1555 |
Sentence |
denotes |
Further, polyclonal antibodies to human calreticulin significantly inhibited the mitogenic activity of fibrinogen B beta chain on human fibroblasts. |
| T18 |
1556-1688 |
Sentence |
denotes |
The present study has shown that cell surface calreticulin binds to the B beta chain of fibrinogen mediating its mitogenic activity. |