| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-31 |
Sentence |
denotes |
ICA512 autoantibody radioassay. |
| TextSentencer_T2 |
32-327 |
Sentence |
denotes |
As part of a general program of screening islet expression libraries we have identified a clone from a lambda gt11 human islet expression library that reacts with human diabetic sera and, upon sequencing, was determined to be the neuroendocrine islet autoantigen ICA512 (islet cell antigen 512). |
| TextSentencer_T3 |
328-526 |
Sentence |
denotes |
In the current communication, we describe the development of a radioassay for autoantibodies to ICA512 (ICA512AA) using in vitro transcribed and translated protein for production of labeled antigen. |
| TextSentencer_T4 |
527-699 |
Sentence |
denotes |
Our initial results indicate that this radioassay is significantly more sensitive than the enzyme-linked immunosorbant assay, which uses a COOH-terminal fragment of ICA512. |
| TextSentencer_T5 |
700-874 |
Sentence |
denotes |
The ICA512AA radioassay uses a 96-well format with membrane separation of antibody bound from free antigen and should be readily adaptable to automated large-scale screening. |
| TextSentencer_T6 |
875-945 |
Sentence |
denotes |
Only 7 microliters of serum is required for triplicate determinations. |
| TextSentencer_T7 |
946-1258 |
Sentence |
denotes |
In order to determine the specificity and sensitivity of this assay and estimate its positive predictive value, we have studied 42 new-onset diabetic patients, 33 first-degree relatives of diabetic patients followed to diabetes, 694 islet cell antibody-negative (ICA-) relatives, and 205 normal control subjects. |
| TextSentencer_T8 |
1259-1438 |
Sentence |
denotes |
Thirty-eight percent of new-onset patients and 48% of relatives followed to diabetes express autoantibodies to ICA512 exceeding the 99th percentile of the normal control subjects. |
| TextSentencer_T9 |
1439-1566 |
Sentence |
denotes |
In contrast, only 1.4% of ICA- first-degree relatives were positive for ICA512 autoantibodies.(ABSTRACT TRUNCATED AT 250 WORDS) |
| T1 |
0-31 |
Sentence |
denotes |
ICA512 autoantibody radioassay. |
| T2 |
32-327 |
Sentence |
denotes |
As part of a general program of screening islet expression libraries we have identified a clone from a lambda gt11 human islet expression library that reacts with human diabetic sera and, upon sequencing, was determined to be the neuroendocrine islet autoantigen ICA512 (islet cell antigen 512). |
| T3 |
328-526 |
Sentence |
denotes |
In the current communication, we describe the development of a radioassay for autoantibodies to ICA512 (ICA512AA) using in vitro transcribed and translated protein for production of labeled antigen. |
| T4 |
527-699 |
Sentence |
denotes |
Our initial results indicate that this radioassay is significantly more sensitive than the enzyme-linked immunosorbant assay, which uses a COOH-terminal fragment of ICA512. |
| T5 |
700-874 |
Sentence |
denotes |
The ICA512AA radioassay uses a 96-well format with membrane separation of antibody bound from free antigen and should be readily adaptable to automated large-scale screening. |
| T6 |
875-945 |
Sentence |
denotes |
Only 7 microliters of serum is required for triplicate determinations. |
| T7 |
946-1258 |
Sentence |
denotes |
In order to determine the specificity and sensitivity of this assay and estimate its positive predictive value, we have studied 42 new-onset diabetic patients, 33 first-degree relatives of diabetic patients followed to diabetes, 694 islet cell antibody-negative (ICA-) relatives, and 205 normal control subjects. |
| T8 |
1259-1438 |
Sentence |
denotes |
Thirty-eight percent of new-onset patients and 48% of relatives followed to diabetes express autoantibodies to ICA512 exceeding the 99th percentile of the normal control subjects. |
| T9 |
1439-1566 |
Sentence |
denotes |
In contrast, only 1.4% of ICA- first-degree relatives were positive for ICA512 autoantibodies.(ABSTRACT TRUNCATED AT 250 WORDS) |
