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PubMed:7565736 JSONTXT

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jnlpba-st-training

Id Subject Object Predicate Lexical cue
T1 0-4 protein denotes PU.1
T2 6-11 protein denotes Spi-1
T3 17-28 protein denotes C/EBP alpha
T4 56-124 DNA denotes granulocyte-macrophage colony-stimulating factor receptor alpha gene
T5 126-149 protein denotes Growth factor receptors
T6 343-364 protein denotes transcription factors
T7 398-475 DNA denotes granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha gene
T8 507-543 DNA denotes human GM-CSF receptor alpha promoter
T9 552-565 DNA denotes reporter gene
T10 607-627 cell_type denotes myelomonocytic cells
T11 720-750 DNA denotes GM-CSF receptor alpha promoter
T12 857-876 DNA denotes reporter constructs
T13 907-934 protein denotes B cell transcription factor
T14 935-939 protein denotes PU.1
T15 1008-1018 DNA denotes CCAAT site
T16 1043-1052 DNA denotes PU.1 site
T17 1134-1164 DNA denotes GM-CSF receptor alpha promoter
T18 1175-1186 protein denotes C/EBP alpha
T19 1200-1230 protein denotes CCAAT/enhancer-binding protein
T20 1232-1237 protein denotes C/EBP
T21 1288-1298 cell_line denotes U937 cells
T22 1330-1339 DNA denotes PU.1 site
T23 1347-1357 DNA denotes C/EBP site
T24 1445-1475 DNA denotes GM-CSF receptor alpha promoter
T25 1488-1508 cell_type denotes myelomonocytic cells
T26 1580-1584 protein denotes PU.1
T27 1641-1646 protein denotes PU-SF
T28 1665-1686 protein denotes GM-CSF receptor alpha
T29 1696-1705 DNA denotes PU.1 site
T30 1770-1774 protein denotes PU.1
T31 1780-1805 DNA denotes myeloid PU.1 binding site
T32 1882-1903 DNA denotes B cell enhancer sites
T33 1937-1941 protein denotes PU.1
T34 1967-1988 cell_type denotes nonmyeloid cell types
T35 2010-2014 protein denotes PU.1
T36 2026-2033 cell_type denotes T cells
T37 2038-2054 cell_type denotes epithelial cells
T38 2069-2084 cell_type denotes erythroid cells
T39 2123-2136 protein denotes PU-SF complex
T40 2146-2150 protein denotes PU.1
T41 2178-2195 DNA denotes myeloid promoters
T42 2234-2243 DNA denotes PU.1 site
T43 2296-2300 protein denotes PU.1
T44 2304-2320 cell_type denotes nonmyeloid cells
T45 2338-2368 DNA denotes GM-CSF receptor alpha promoter
T46 2386-2407 protein denotes amino-terminal region
T47 2411-2415 protein denotes PU.1
T48 2449-2454 protein denotes PU-SF
T49 2542-2555 protein denotes PU-SF complex
T50 2608-2638 DNA denotes GM-CSF receptor alpha promoter
T51 2669-2680 protein denotes C/EBP alpha
T52 2701-2731 DNA denotes GM-CSF receptor alpha promoter
T53 2735-2751 cell_type denotes nonmyeloid cells

pubmed-sentences-benchmark

Id Subject Object Predicate Lexical cue
S1 0-125 Sentence denotes PU.1 (Spi-1) and C/EBP alpha regulate expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene.
S2 126-190 Sentence denotes Growth factor receptors play an important role in hematopoiesis.
S3 191-476 Sentence denotes In order to further understand the mechanisms directing the expression of these key regulators of hematopoiesis, we initiated a study investigating the transcription factors activating the expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha gene.
S4 477-715 Sentence denotes Here, we demonstrate that the human GM-CSF receptor alpha promoter directs reporter gene activity in a tissue-specific fashion in myelomonocytic cells, which correlates with its expression pattern as analyzed by reverse transcription PCR.
S5 716-877 Sentence denotes The GM-CSF receptor alpha promoter contains an important functional site between positions -53 and -41 as identified by deletion analysis of reporter constructs.
S6 878-972 Sentence denotes We show that the myeloid and B cell transcription factor PU.1 binds specifically to this site.
S7 973-1174 Sentence denotes Furthermore, we demonstrate that a CCAAT site located upstream of the PU.1 site between positions -70 and -54 is involved in positive-negative regulation of the GM-CSF receptor alpha promoter activity.
S8 1175-1299 Sentence denotes C/EBP alpha is the major CCAAT/enhancer-binding protein (C/EBP) form binding to this site in nuclear extracts of U937 cells.
S9 1300-1514 Sentence denotes Point mutations of either the PU.1 site or the C/EBP site that abolish the binding of the respective factors result in a significant decrease of GM-CSF receptor alpha promoter activity in myelomonocytic cells only.
S10 1515-1706 Sentence denotes Furthermore, we demonstrate that in myeloid and B cell extracts, PU.1 forms a novel, specific, more slowly migrating complex (PU-SF) when binding the GM-CSF receptor alpha promoter PU.1 site.
S11 1707-1806 Sentence denotes This is the first demonstration of a specific interaction with PU.1 on a myeloid PU.1 binding site.
S12 1807-2085 Sentence denotes The novel complex is distinct from that described previously as binding to B cell enhancer sites and can be formed by addition of PU.1 to extracts from certain nonmyeloid cell types which do not express PU.1, including T cells and epithelial cells, but not from erythroid cells.
S13 2086-2281 Sentence denotes Furthermore, we demonstrate that the PU-SF complex binds to PU.1 sites found on a number of myeloid promoters, and its formation requires an intact PU.1 site adjacent to a single-stranded region.
S14 2282-2369 Sentence denotes Expression of PU.1 in nonmyeloid cells can activate the GM-CSF receptor alpha promoter.
S15 2370-2639 Sentence denotes Deletion of the amino-terminal region of PU.1 results in a failure to form the PU-SF complex and in a concomitant loss of transactivation, suggesting that formation of the PU-SF complex is of functional importance for the activity of the GM-CSF receptor alpha promoter.
S16 2640-2752 Sentence denotes Finally, we demonstrate that C/EBP alpha can also active the GM-CSF receptor alpha promoter in nonmyeloid cells.
S17 2753-3015 Sentence denotes These results suggest that PU.1 and C/EBP alpha direct the cell-type-specific expression of GM-CSF receptor alpha, further establish the role of PU.1 as a key regulator of hematopoiesis, and point to C/EBP alpha as an additional important factor in this process.

genia-medco-coref

Id Subject Object Predicate Lexical cue
C1 0-12 NP denotes PU.1 (Spi-1)
C2 17-28 NP denotes C/EBP alpha
C3 38-124 NP denotes expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene
C4 126-149 NP denotes Growth factor receptors
C5 176-189 NP denotes hematopoiesis
C7 289-302 NP denotes hematopoiesis
C6 265-302 NP denotes these key regulators of hematopoiesis
C8 376-475 NP denotes the expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha gene
C9 503-543 NP denotes the human GM-CSF receptor alpha promoter
C10 651-654 NP denotes its
C11 716-750 NP denotes The GM-CSF receptor alpha promoter
C12 760-876 NP denotes an important functional site between positions -53 and -41 as identified by deletion analysis of reporter constructs
C13 891-939 NP denotes the myeloid and B cell transcription factor PU.1
C14 962-971 NP denotes this site
C16 1039-1052 NP denotes the PU.1 site
C15 1006-1082 NP denotes a CCAAT site located upstream of the PU.1 site between positions -70 and -54
C17 1130-1173 NP denotes the GM-CSF receptor alpha promoter activity
C18 1175-1186 NP denotes C/EBP alpha
C19 1255-1264 NP denotes this site
C20 1326-1339 NP denotes the PU.1 site
C21 1343-1357 NP denotes the C/EBP site
C22 1445-1484 NP denotes GM-CSF receptor alpha promoter activity
C23 1580-1584 NP denotes PU.1
C24 1591-1647 NP denotes a novel, specific, more slowly migrating complex (PU-SF)
C25 1770-1774 NP denotes PU.1
C26 1807-1824 NP denotes The novel complex
C27 1842-1846 NP denotes that
C28 1937-1941 NP denotes PU.1
C29 1959-1988 NP denotes certain nonmyeloid cell types
C30 1989-1994 NP denotes which
C31 2010-2014 NP denotes PU.1
C32 2119-2136 NP denotes the PU-SF complex
C33 2146-2156 NP denotes PU.1 sites
C34 2201-2204 NP denotes its
C35 2296-2300 NP denotes PU.1
C36 2304-2320 NP denotes nonmyeloid cells
C37 2334-2368 NP denotes the GM-CSF receptor alpha promoter
C38 2411-2415 NP denotes PU.1
C39 2445-2462 NP denotes the PU-SF complex
C40 2538-2555 NP denotes the PU-SF complex
C42 2604-2638 NP denotes the GM-CSF receptor alpha promoter
C41 2588-2638 NP denotes the activity of the GM-CSF receptor alpha promoter
C43 2669-2680 NP denotes C/EBP alpha
C44 2697-2731 NP denotes the GM-CSF receptor alpha promoter
C45 2735-2751 NP denotes nonmyeloid cells
R1 C7 C5 coref-ident hematopoiesis,hematopoiesis
R2 C6 C4 coref-ident these key regulators of hematopoiesis,Growth factor receptors
R3 C8 C3 coref-ident the expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha gene,expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene
R4 C10 C9 coref-pron its,the human GM-CSF receptor alpha promoter
R5 C11 C9 coref-ident The GM-CSF receptor alpha promoter,the human GM-CSF receptor alpha promoter
R6 C13 C1 coref-ident the myeloid and B cell transcription factor PU.1,PU.1 (Spi-1)
R7 C14 C12 coref-ident this site,an important functional site between positions -53 and -41 as identified by deletion analysis of reporter constructs
R8 C16 C14 coref-ident the PU.1 site,this site
R9 C18 C2 coref-ident C/EBP alpha,C/EBP alpha
R10 C19 C15 coref-ident this site,a CCAAT site located upstream of the PU.1 site between positions -70 and -54
R11 C20 C16 coref-ident the PU.1 site,the PU.1 site
R12 C21 C19 coref-ident the C/EBP site,this site
R13 C22 C17 coref-ident GM-CSF receptor alpha promoter activity,the GM-CSF receptor alpha promoter activity
R14 C23 C13 coref-ident PU.1,the myeloid and B cell transcription factor PU.1
R15 C25 C23 coref-ident PU.1,PU.1
R16 C26 C24 coref-ident The novel complex,"a novel, specific, more slowly migrating complex (PU-SF)"
R17 C27 C26 coref-pron that,The novel complex
R18 C28 C25 coref-ident PU.1,PU.1
R19 C30 C29 coref-relat which,certain nonmyeloid cell types
R20 C31 C28 coref-ident PU.1,PU.1
R21 C32 C26 coref-ident the PU-SF complex,The novel complex
R22 C33 C20 coref-ident PU.1 sites,the PU.1 site
R23 C34 C32 coref-pron its,the PU-SF complex
R24 C35 C31 coref-ident PU.1,PU.1
R25 C37 C11 coref-ident the GM-CSF receptor alpha promoter,The GM-CSF receptor alpha promoter
R26 C38 C35 coref-ident PU.1,PU.1
R27 C39 C32 coref-ident the PU-SF complex,the PU-SF complex
R28 C40 C39 coref-ident the PU-SF complex,the PU-SF complex
R29 C42 C37 coref-ident the GM-CSF receptor alpha promoter,the GM-CSF receptor alpha promoter
R30 C41 C22 coref-ident the activity of the GM-CSF receptor alpha promoter,GM-CSF receptor alpha promoter activity
R31 C43 C18 coref-ident C/EBP alpha,C/EBP alpha
R32 C44 C42 coref-ident the GM-CSF receptor alpha promoter,the GM-CSF receptor alpha promoter
R33 C45 C36 coref-ident nonmyeloid cells,nonmyeloid cells

GENIAcorpus

Id Subject Object Predicate Lexical cue
T1 0-4 protein_molecule denotes PU.1
T2 6-11 protein_molecule denotes Spi-1
T3 17-28 protein_molecule denotes C/EBP alpha
T4 56-113 protein_molecule denotes granulocyte-macrophage colony-stimulating factor receptor
T5 126-149 protein_family_or_group denotes Growth factor receptors
T6 176-189 other_name denotes hematopoiesis
T7 289-302 other_name denotes hematopoiesis
T8 343-364 protein_family_or_group denotes transcription factors
T9 398-446 protein_molecule denotes granulocyte-macrophage colony-stimulating factor
T10 448-454 protein_molecule denotes GM-CSF
T11 507-512 DNA_domain_or_region denotes human
T12 513-519 protein_molecule denotes GM-CSF
T13 552-565 DNA_family_or_group denotes reporter gene
T14 580-603 other_name denotes tissue-specific fashion
T15 607-627 cell_type denotes myelomonocytic cells
T16 689-714 other_name denotes reverse transcription PCR
T17 720-726 protein_molecule denotes GM-CSF
T18 857-876 DNA_family_or_group denotes reporter constructs
T19 907-934 protein_family_or_group denotes B cell transcription factor
T20 935-939 protein_molecule denotes PU.1
T21 1008-1018 DNA_domain_or_region denotes CCAAT site
T22 1043-1047 protein_molecule denotes PU.1
T23 1098-1126 other_name denotes positive-negative regulation
T24 1134-1140 protein_molecule denotes GM-CSF
T25 1175-1186 protein_molecule denotes C/EBP alpha
T26 1200-1214 DNA_domain_or_region denotes CCAAT/enhancer
T27 1232-1237 protein_family_or_group denotes C/EBP
T28 1268-1284 cell_component denotes nuclear extracts
T29 1288-1298 cell_line denotes U937 cells
T30 1330-1334 protein_molecule denotes PU.1
T31 1347-1352 protein_molecule denotes C/EBP
T32 1445-1451 protein_molecule denotes GM-CSF
T33 1488-1508 cell_type denotes myelomonocytic cells
T34 1580-1584 protein_molecule denotes PU.1
T35 1641-1646 protein_complex denotes PU-SF
T36 1665-1671 protein_molecule denotes GM-CSF
T37 1696-1700 protein_molecule denotes PU.1
T38 1770-1774 protein_molecule denotes PU.1
T39 1780-1787 DNA_domain_or_region denotes myeloid
T40 1788-1792 protein_molecule denotes PU.1
T41 1882-1903 DNA_domain_or_region denotes B cell enhancer sites
T42 1937-1941 protein_molecule denotes PU.1
T43 1967-1988 cell_type denotes nonmyeloid cell types
T44 2010-2014 protein_molecule denotes PU.1
T45 2026-2033 cell_type denotes T cells
T46 2038-2054 cell_type denotes epithelial cells
T47 2069-2084 cell_type denotes erythroid cells
T48 2123-2128 protein_complex denotes PU-SF
T49 2146-2150 protein_molecule denotes PU.1
T50 2178-2195 DNA_family_or_group denotes myeloid promoters
T51 2234-2238 protein_molecule denotes PU.1
T52 2296-2300 protein_molecule denotes PU.1
T53 2304-2320 cell_type denotes nonmyeloid cells
T54 2338-2344 protein_molecule denotes GM-CSF
T55 2386-2407 protein_domain_or_region denotes amino-terminal region
T56 2411-2415 protein_molecule denotes PU.1
T57 2449-2454 protein_complex denotes PU-SF
T58 2542-2547 protein_complex denotes PU-SF
T59 2608-2614 protein_molecule denotes GM-CSF
T60 2669-2674 protein_molecule denotes C/EBP
T61 2701-2707 protein_molecule denotes GM-CSF
T62 2735-2751 cell_type denotes nonmyeloid cells