Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-103 |
Sentence |
denotes |
The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments. |
T2 |
104-469 |
Sentence |
denotes |
The low molecular weight GTP-binding proteins RhoA, RhoB, and RhoC are characterized as specific substrates for the ADP-ribosyltransferase C3 from Clostridium botulinum and are supposed to be involved in the organization of the microfilamental network and transformation. rhoB is known to be immediate-early inducible by growth factors and protein-tyrosine kinases. |
T3 |
470-657 |
Sentence |
denotes |
Since increasing evidence indicates overlapping of growth factor- and UV-induced signal pathways, we studied the effect of UV light and other genotoxic agents on early rhoB transcription. |
T4 |
658-753 |
Sentence |
denotes |
Within 30 min after UV irradiation of NIH3T3 cells, the amount of rhoB mRNA increased 3-4-fold. |
T5 |
754-870 |
Sentence |
denotes |
Elevated rhoB mRNA was accompanied by an increase in RhoB protein, as detected by C3-mediated [32P]ADP-ribosylation. |
T6 |
871-1037 |
Sentence |
denotes |
The transcription inhibitor actinomycin D prevented the UV-induced increase in rhoB mRNA and proved rhoB mRNA to be unstable with a half-life of approximately 20 min. |
T7 |
1038-1118 |
Sentence |
denotes |
Transcriptional activation of rhoB by UV light was confirmed by run-on analysis. |
T8 |
1119-1236 |
Sentence |
denotes |
The increase in rhoB mRNA after UV irradiation was prevented by inhibitors of protein kinase A (H9) and C (H7, Gö18). |
T9 |
1237-1313 |
Sentence |
denotes |
The tyrosine kinase inhibitor genistein did not affect UV induction of rhoB. |
T10 |
1314-1411 |
Sentence |
denotes |
In addition to UV, N-methyl-N-nitrosourea and the cytostatic drug cisplatin evoked rhoB response. |
T11 |
1412-1581 |
Sentence |
denotes |
Cycloheximide was likewise effective in increasing the amount of rhoB mRNA, whereas Bt2cAMP, 12-O-tetradecanoylphorbol-13-acetate, and retinoic acid were without effect. |
T12 |
1582-1718 |
Sentence |
denotes |
Prior down-regulation of signaling by 12-O-tetradecanoylphorbol-13-acetate and serum pretreatment reduced UV-stimulated rhoB expression. |
T13 |
1719-1865 |
Sentence |
denotes |
The data indicate that rhoB represents a novel DNA damage-inducible function involved in early steps of signal transduction upon genotoxic stress. |
T1 |
0-103 |
Sentence |
denotes |
The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments. |
T2 |
104-469 |
Sentence |
denotes |
The low molecular weight GTP-binding proteins RhoA, RhoB, and RhoC are characterized as specific substrates for the ADP-ribosyltransferase C3 from Clostridium botulinum and are supposed to be involved in the organization of the microfilamental network and transformation. rhoB is known to be immediate-early inducible by growth factors and protein-tyrosine kinases. |
T3 |
470-657 |
Sentence |
denotes |
Since increasing evidence indicates overlapping of growth factor- and UV-induced signal pathways, we studied the effect of UV light and other genotoxic agents on early rhoB transcription. |
T4 |
658-753 |
Sentence |
denotes |
Within 30 min after UV irradiation of NIH3T3 cells, the amount of rhoB mRNA increased 3-4-fold. |
T5 |
754-870 |
Sentence |
denotes |
Elevated rhoB mRNA was accompanied by an increase in RhoB protein, as detected by C3-mediated [32P]ADP-ribosylation. |
T6 |
871-1037 |
Sentence |
denotes |
The transcription inhibitor actinomycin D prevented the UV-induced increase in rhoB mRNA and proved rhoB mRNA to be unstable with a half-life of approximately 20 min. |
T7 |
1038-1118 |
Sentence |
denotes |
Transcriptional activation of rhoB by UV light was confirmed by run-on analysis. |
T8 |
1119-1236 |
Sentence |
denotes |
The increase in rhoB mRNA after UV irradiation was prevented by inhibitors of protein kinase A (H9) and C (H7, Gö18). |
T9 |
1237-1313 |
Sentence |
denotes |
The tyrosine kinase inhibitor genistein did not affect UV induction of rhoB. |
T10 |
1314-1411 |
Sentence |
denotes |
In addition to UV, N-methyl-N-nitrosourea and the cytostatic drug cisplatin evoked rhoB response. |
T11 |
1412-1581 |
Sentence |
denotes |
Cycloheximide was likewise effective in increasing the amount of rhoB mRNA, whereas Bt2cAMP, 12-O-tetradecanoylphorbol-13-acetate, and retinoic acid were without effect. |
T12 |
1582-1718 |
Sentence |
denotes |
Prior down-regulation of signaling by 12-O-tetradecanoylphorbol-13-acetate and serum pretreatment reduced UV-stimulated rhoB expression. |
T13 |
1719-1865 |
Sentence |
denotes |
The data indicate that rhoB represents a novel DNA damage-inducible function involved in early steps of signal transduction upon genotoxic stress. |