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Id Subject Object Predicate Lexical cue
T1 0-85 Sentence denotes Role of phosphorylation in agonist-promoted beta 2-adrenergic receptor sequestration.
T2 86-151 Sentence denotes Rescue of a sequestration-defective mutant receptor by beta ARK1.
T3 152-253 Sentence denotes The beta 2-adrenergic receptor (beta 2AR) belongs to the large family of G protein-coupled receptors.
T4 254-469 Sentence denotes Mutation of tyrosine residue 326 to an alanine resulted in a beta 2AR mutant (beta 2AR-Y326A) that was defective in its ability to sequester and was less well coupled to adenylyl cyclase than the wild-type beta 2AR.
T5 470-585 Sentence denotes However, this mutant receptor not only desensitized in response to agonist stimulation but down-regulated normally.
T6 586-768 Sentence denotes In an attempt to understand the basis for the properties of this mutant, we have examined the ability of this regulation-defective mutant to undergo agonist-mediated phosphorylation.
T7 769-888 Sentence denotes When expressed in 293 cells, the maximal response for phosphorylation of the beta 2AR-Y326A mutant was impaired by 75%.
T8 889-1225 Sentence denotes Further characterization of this phosphorylation, using either forskolin stimulation or phosphorylation site-deficient beta 2AR-Y326A mutants, demonstrated that the beta 2AR-Y326A mutant can be phosphorylated by cAMP-dependent protein kinase (PKA) but does not serve as a substrate for the beta-adrenergic receptor kinase 1 (beta ARK1).
T9 1226-1534 Sentence denotes However, overexpression of beta ARK1 led to the agonist-dependent phosphorylation of the beta 2AR-Y326A mutant and rescue of its sequestration. beta ARK1-mediated rescue of beta 2AR-Y326A sequestration could be prevented by mutating putative beta ARK phosphorylation sites, but not PKA phosphorylation sites.
T10 1535-1713 Sentence denotes In addition, both sequestration and phosphorylation of the wild-type beta 2AR could be attenuated by overexpressing a dominant-negative mutant of beta ARK1 (C20 beta ARK1-K220M).
T11 1714-1834 Sentence denotes These findings implicate a role for beta ARK1-mediated phosphorylation in facilitating wild-type beta 2AR sequestration.
T1 0-85 Sentence denotes Role of phosphorylation in agonist-promoted beta 2-adrenergic receptor sequestration.
T2 86-151 Sentence denotes Rescue of a sequestration-defective mutant receptor by beta ARK1.
T3 152-253 Sentence denotes The beta 2-adrenergic receptor (beta 2AR) belongs to the large family of G protein-coupled receptors.
T4 254-469 Sentence denotes Mutation of tyrosine residue 326 to an alanine resulted in a beta 2AR mutant (beta 2AR-Y326A) that was defective in its ability to sequester and was less well coupled to adenylyl cyclase than the wild-type beta 2AR.
T5 470-585 Sentence denotes However, this mutant receptor not only desensitized in response to agonist stimulation but down-regulated normally.
T6 586-768 Sentence denotes In an attempt to understand the basis for the properties of this mutant, we have examined the ability of this regulation-defective mutant to undergo agonist-mediated phosphorylation.
T7 769-888 Sentence denotes When expressed in 293 cells, the maximal response for phosphorylation of the beta 2AR-Y326A mutant was impaired by 75%.
T8 889-1225 Sentence denotes Further characterization of this phosphorylation, using either forskolin stimulation or phosphorylation site-deficient beta 2AR-Y326A mutants, demonstrated that the beta 2AR-Y326A mutant can be phosphorylated by cAMP-dependent protein kinase (PKA) but does not serve as a substrate for the beta-adrenergic receptor kinase 1 (beta ARK1).
T9 1226-1534 Sentence denotes However, overexpression of beta ARK1 led to the agonist-dependent phosphorylation of the beta 2AR-Y326A mutant and rescue of its sequestration. beta ARK1-mediated rescue of beta 2AR-Y326A sequestration could be prevented by mutating putative beta ARK phosphorylation sites, but not PKA phosphorylation sites.
T10 1535-1713 Sentence denotes In addition, both sequestration and phosphorylation of the wild-type beta 2AR could be attenuated by overexpressing a dominant-negative mutant of beta ARK1 (C20 beta ARK1-K220M).
T11 1714-1834 Sentence denotes These findings implicate a role for beta ARK1-mediated phosphorylation in facilitating wild-type beta 2AR sequestration.