PubMed:7540078 JSON TXT

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Glycan-Motif

GlyCosmos6-Glycan-Motif-Image

Glycosmos6-GlycoEpitope

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":919,"end":923},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"T2","span":{"begin":1038,"end":1042},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"T3","span":{"begin":1496,"end":1500},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"T4","span":{"begin":1517,"end":1521},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyCosmos6-Glycan-Motif-Structure

Glycosmos6-MAT

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"T2","span":{"begin":265,"end":271},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"T3","span":{"begin":445,"end":450},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T4","span":{"begin":445,"end":450},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T5","span":{"begin":550,"end":556},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"T6","span":{"begin":704,"end":709},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T7","span":{"begin":704,"end":709},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T8","span":{"begin":753,"end":759},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlycoBiology-FMA

uniprot-human

uniprot-mouse

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":1021,"end":1023},"obj":"http://www.uniprot.org/uniprot/P04441"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlycoBiology-NCBITAXON

GO-BP

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":66,"end":81},"obj":"http://purl.obolibrary.org/obo/GO_0007283"},{"id":"T2","span":{"begin":279,"end":294},"obj":"http://purl.obolibrary.org/obo/GO_0007283"},{"id":"T3","span":{"begin":781,"end":796},"obj":"http://purl.obolibrary.org/obo/GO_0007283"},{"id":"T4","span":{"begin":1558,"end":1573},"obj":"http://purl.obolibrary.org/obo/GO_0007283"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T1","span":{"begin":326,"end":334},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T2","span":{"begin":265,"end":271},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T3","span":{"begin":550,"end":556},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T4","span":{"begin":753,"end":759},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlycoBiology-MAT

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"T2","span":{"begin":265,"end":271},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"T3","span":{"begin":445,"end":450},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T4","span":{"begin":445,"end":450},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T5","span":{"begin":550,"end":556},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"T6","span":{"begin":704,"end":709},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T7","span":{"begin":704,"end":709},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T8","span":{"begin":753,"end":759},"obj":"http://purl.obolibrary.org/obo/MAT_0000132"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyTouCan-IUPAC

sentences

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":119,"end":404},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":405,"end":597},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":598,"end":797},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":798,"end":1675},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1676,"end":1957},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1958,"end":2017},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T2","span":{"begin":119,"end":404},"obj":"Sentence"},{"id":"T3","span":{"begin":405,"end":597},"obj":"Sentence"},{"id":"T4","span":{"begin":598,"end":797},"obj":"Sentence"},{"id":"T5","span":{"begin":798,"end":1675},"obj":"Sentence"},{"id":"T6","span":{"begin":1676,"end":1957},"obj":"Sentence"},{"id":"T7","span":{"begin":1958,"end":2017},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T2","span":{"begin":119,"end":404},"obj":"Sentence"},{"id":"T3","span":{"begin":405,"end":597},"obj":"Sentence"},{"id":"T4","span":{"begin":598,"end":797},"obj":"Sentence"},{"id":"T5","span":{"begin":798,"end":1675},"obj":"Sentence"},{"id":"T6","span":{"begin":1676,"end":1957},"obj":"Sentence"},{"id":"T7","span":{"begin":1958,"end":2017},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

Lectin

{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":1766,"end":1769},"obj":"https://acgg.asia/db/lfdb/LfDB0050"},{"id":"Lectin_T2","span":{"begin":1940,"end":1943},"obj":"https://acgg.asia/db/lfdb/LfDB0050"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlycoBiology-Epitope

{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":919,"end":923},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":1038,"end":1042},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"PD-GlycoEpitope-B_T3","span":{"begin":1496,"end":1500},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"PD-GlycoEpitope-B_T4","span":{"begin":1517,"end":1521},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

performance-test

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":52,"end":58},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":265,"end":271},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":550,"end":556},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":753,"end":759},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

Anatomy-MAT

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"Body_part"},{"id":"T2","span":{"begin":265,"end":271},"obj":"Body_part"},{"id":"T3","span":{"begin":445,"end":450},"obj":"Body_part"},{"id":"T5","span":{"begin":550,"end":556},"obj":"Body_part"},{"id":"T6","span":{"begin":704,"end":709},"obj":"Body_part"},{"id":"T8","span":{"begin":753,"end":759},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A4","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"A6","pred":"mat_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A7","pred":"mat_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A8","pred":"mat_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MAT_0000132"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

Glycan-GlyCosmos

GlyCosmos-GlycoEpitope

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":919,"end":923},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":1038,"end":1042},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":1496,"end":1500},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":1517,"end":1521},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0056"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyCosmos15-CL

{"project":"GlyCosmos15-CL","denotations":[{"id":"T1","span":{"begin":445,"end":450},"obj":"Cell"},{"id":"T2","span":{"begin":704,"end":709},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000019"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000019"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyCosmos15-UBERON

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"Body_part"},{"id":"T2","span":{"begin":265,"end":271},"obj":"Body_part"},{"id":"T3","span":{"begin":445,"end":450},"obj":"Body_part"},{"id":"T5","span":{"begin":550,"end":556},"obj":"Body_part"},{"id":"T6","span":{"begin":704,"end":709},"obj":"Body_part"},{"id":"T8","span":{"begin":753,"end":759},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A7","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyCosmos15-MAT

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"Body_part"},{"id":"T2","span":{"begin":265,"end":271},"obj":"Body_part"},{"id":"T3","span":{"begin":445,"end":450},"obj":"Body_part"},{"id":"T5","span":{"begin":550,"end":556},"obj":"Body_part"},{"id":"T6","span":{"begin":704,"end":709},"obj":"Body_part"},{"id":"T8","span":{"begin":753,"end":759},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A4","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000132"},{"id":"A6","pred":"mat_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A7","pred":"mat_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A8","pred":"mat_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MAT_0000132"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

sentences

GlyCosmos15-Sentences

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T2","span":{"begin":119,"end":404},"obj":"Sentence"},{"id":"T3","span":{"begin":405,"end":597},"obj":"Sentence"},{"id":"T4","span":{"begin":598,"end":797},"obj":"Sentence"},{"id":"T5","span":{"begin":798,"end":1675},"obj":"Sentence"},{"id":"T6","span":{"begin":1676,"end":1957},"obj":"Sentence"},{"id":"T7","span":{"begin":1958,"end":2017},"obj":"Sentence"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyCosmos15-Glycan

GlyCosmos15-GlycoEpitope

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":919,"end":923},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":1038,"end":1042},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":1496,"end":1500},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":1517,"end":1521},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0056"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

GlyCosmos15-Lectin-Jamboree

{"project":"GlyCosmos15-Lectin-Jamboree","denotations":[{"id":"T1","span":{"begin":1766,"end":1769},"obj":"Lectin"},{"id":"T2","span":{"begin":1940,"end":1943},"obj":"Lectin"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"GL_005239"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"GL_005239"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

Anatomy-UBERON

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":52,"end":58},"obj":"Body_part"},{"id":"T2","span":{"begin":265,"end":271},"obj":"Body_part"},{"id":"T3","span":{"begin":445,"end":450},"obj":"Body_part"},{"id":"T5","span":{"begin":550,"end":556},"obj":"Body_part"},{"id":"T6","span":{"begin":704,"end":709},"obj":"Body_part"},{"id":"T8","span":{"begin":753,"end":759},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A7","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}

CL-cell

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":445,"end":450},"obj":"Cell"},{"id":"T2","span":{"begin":704,"end":709},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000019"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000019"}],"text":"Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.\nThe developmental expression of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid-containing glycosphingolipids (KDN-gangliosides) in rainbow trout testis during spermatogenesis was studied using a monoclonal antibody, mAb.kdn3G, which recognizes the KDN alpha 2--\u003e3Gal beta 1--\u003eepitope. A major KDN-ganglioside found in mature sperm, (KDN)GM3, KDN alpha 2--\u003e3Gal beta 1--\u003e4Glc beta 1--\u003eCer (where Cer is ceramide), was expressed in testis throughout all stages of its maturation. On the contrary, four new KDN-gangliosides which were reactive with mAb.kdn3G were not detected in mature sperm, although they were identified in immature testis and expressed during spermatogenesis. The structures of these KDN-gangliosides were established by chemical, enzymatic and immunochemical methods as: (i) (KDN)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (ii) (KDN, Neu5Ac)GD1a, KDN alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(Neu5Ac alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer and Neu5Ac alpha 2--\u003e3Gal beta 1--\u003e3GalNAc beta 1--\u003e4(KDN alpha 2--\u003e3)Gal beta 1--\u003e4Glc beta 1--\u003eCer; (iii) (KDN) GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(KDN alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer; and (iv) (KDN,Neu5Ac)GD1 alpha, KDN alpha 2--\u003e3Gal beta 1--\u003e3(Neu5Ac alpha 2--\u003e6)GalNAc beta 1--\u003e4Gal beta 1--\u003e4Glc beta 1--\u003eCer. (KDN)GD1a and (KDN,Neu5Ac)GD1a first appeared at an early stage of spermatogenesis, but (KDN)GD1 alpha and (KDN,Neu5Ac)GD1 alpha were not expressed until 2 months prior to spermiation. While (KDN)GM3 was previously shown to contain only 4-sphingenine (d18:1) acylated with a C16:0 fatty acid, the new KDN-gangliosides discovered in this study were composed of 4-hydroxysphinganine (t18:0) or 4-sphingenine (d18:1), and were acylated with a C24:1 or C16:0 fatty acid. A possible function of these KDN-gangliosides is suggested."}