| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-60 |
Sentence |
denotes |
A novel form of dipeptidylpeptidase IV found in human serum. |
| T2 |
61-162 |
Sentence |
denotes |
Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26). |
| T3 |
163-416 |
Sentence |
denotes |
Human CD26, a Type II membrane glycoprotein with intrinsic dipeptidylpeptidase IV (DPPIV) activity and ability to bind adenosine deaminase type I (ADA-1), is expressed on epithelial cells constitutively, but on T lymphocytes its expression is regulated. |
| T4 |
417-595 |
Sentence |
denotes |
A soluble form of CD26/DPPIV has been described in plasma and related to immunological status, but it has been defined by the presence of DPPIV activity rather than by isolation. |
| T5 |
596-829 |
Sentence |
denotes |
Using nondenaturing chromatographic techniques followed by nondenaturing native preparative electrophoresis, we obtained a homogeneous preparation of soluble serum DPPIV and compared it with a recombinant soluble CD26/DPPIV (rsCD26). |
| T6 |
830-989 |
Sentence |
denotes |
We show that serum DPPIV is a monomer of 175 kDa in contrast to rsCD26 of 105-110 kDa, that it exists as a trimer, and that it is probably a serine proteinase. |
| T7 |
990-1155 |
Sentence |
denotes |
Deglycosylation removed N-linked sugar from both serum DPPIV and rsCD26; no O-linked glycosylation was observed, revealing a protein core of 130 kDa for serum DPPIV. |
| T8 |
1156-1307 |
Sentence |
denotes |
The large serum form expresses functional DPPIV activity with substrate and inhibitor specificities and pH activity profile similar to those of rsCD26. |
| T9 |
1308-1447 |
Sentence |
denotes |
Epitope analysis showed that monoclonal antibodies against five epitopes expressed by rsCD26 also bound, but more weakly, with serum DPPIV. |
| T10 |
1448-1593 |
Sentence |
denotes |
Analysis of peptides after limiting proteolysis and N-terminal sequences reveals no homology with rsCD26 but some identity with other peptidases. |
| T11 |
1594-1688 |
Sentence |
denotes |
Unlike rsCD26, the serum form does not bind ADA-1 and has no ADA-1 already associated with it. |
| T12 |
1689-1754 |
Sentence |
denotes |
Similarly to rsCD26, serum DPPIV is a potent T cell costimulator. |
| T13 |
1755-1854 |
Sentence |
denotes |
We conclude that the serum form of DPPIV is unique and is not a breakdown product of membrane CD26. |
| T14 |
1855-1981 |
Sentence |
denotes |
The conservation of DPPIV activity and five epitopes specific to rsCD26 suggest, however, a significant structural similarity. |
| T1 |
0-60 |
Sentence |
denotes |
A novel form of dipeptidylpeptidase IV found in human serum. |
| T2 |
61-162 |
Sentence |
denotes |
Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26). |
| T3 |
163-416 |
Sentence |
denotes |
Human CD26, a Type II membrane glycoprotein with intrinsic dipeptidylpeptidase IV (DPPIV) activity and ability to bind adenosine deaminase type I (ADA-1), is expressed on epithelial cells constitutively, but on T lymphocytes its expression is regulated. |
| T4 |
417-595 |
Sentence |
denotes |
A soluble form of CD26/DPPIV has been described in plasma and related to immunological status, but it has been defined by the presence of DPPIV activity rather than by isolation. |
| T5 |
596-829 |
Sentence |
denotes |
Using nondenaturing chromatographic techniques followed by nondenaturing native preparative electrophoresis, we obtained a homogeneous preparation of soluble serum DPPIV and compared it with a recombinant soluble CD26/DPPIV (rsCD26). |
| T6 |
830-989 |
Sentence |
denotes |
We show that serum DPPIV is a monomer of 175 kDa in contrast to rsCD26 of 105-110 kDa, that it exists as a trimer, and that it is probably a serine proteinase. |
| T7 |
990-1155 |
Sentence |
denotes |
Deglycosylation removed N-linked sugar from both serum DPPIV and rsCD26; no O-linked glycosylation was observed, revealing a protein core of 130 kDa for serum DPPIV. |
| T8 |
1156-1307 |
Sentence |
denotes |
The large serum form expresses functional DPPIV activity with substrate and inhibitor specificities and pH activity profile similar to those of rsCD26. |
| T9 |
1308-1447 |
Sentence |
denotes |
Epitope analysis showed that monoclonal antibodies against five epitopes expressed by rsCD26 also bound, but more weakly, with serum DPPIV. |
| T10 |
1448-1593 |
Sentence |
denotes |
Analysis of peptides after limiting proteolysis and N-terminal sequences reveals no homology with rsCD26 but some identity with other peptidases. |
| T11 |
1594-1688 |
Sentence |
denotes |
Unlike rsCD26, the serum form does not bind ADA-1 and has no ADA-1 already associated with it. |
| T12 |
1689-1754 |
Sentence |
denotes |
Similarly to rsCD26, serum DPPIV is a potent T cell costimulator. |
| T13 |
1755-1854 |
Sentence |
denotes |
We conclude that the serum form of DPPIV is unique and is not a breakdown product of membrane CD26. |
| T14 |
1855-1981 |
Sentence |
denotes |
The conservation of DPPIV activity and five epitopes specific to rsCD26 suggest, however, a significant structural similarity. |