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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-131 Sentence denotes Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters.
T2 132-269 Sentence denotes Human eosinophil major basic protein (MBP) is one of the principal mediators of injury to parasites and tissues in allergic inflammation.
T3 270-389 Sentence denotes MBP is stored in eosinophil crystalloid granules and released with other granule constituents during eosinophil action.
T4 390-565 Sentence denotes Previous studies have identified an MBP gene promoter that generates a 1.0 kb mRNA transcript encoding MBP preproprotein which undergoes processing to the mature storage form.
T5 566-725 Sentence denotes To investigate how the MBP gene is regulated, we have examined the identity and levels of the MBP transcripts both in precursor cells and in blood eosinophils.
T6 726-869 Sentence denotes It was found that the gene was expressed from two upstream promoters, a distal promoter P1 in addition to the previously described promoter P2.
T7 870-1058 Sentence denotes Evidence for the second promoter was initially provided by isolation from a human HL-60 leukaemic cell cDNA library of a novel 1.6 kb MBP cDNA that was distinct from the known 1.0 kb cDNA.
T8 1059-1241 Sentence denotes The complete nucleotide sequence of the 1.6 kb cDNA was determined, and showed that the two cDNAs had identical coding and 3' untranslated regions but differed in their 5' sequences.
T9 1242-1423 Sentence denotes By isolating and sequencing MBP genomic clones from an arrayed chromosome 11 library, it was demonstrated that the MBP gene is composed of nine upstream exons and five coding exons.
T10 1424-1587 Sentence denotes The 1.6 and 1.0 kb cDNAs arise by differential splicing of alternate MBP transcripts from promoters P1 and P2 respectively, located 32 kb apart in the genomic DNA.
T11 1588-1711 Sentence denotes Primer extension analysis identified two transcription start sites at P1, neither associated with a typical TATA box motif.
T12 1712-1906 Sentence denotes Northern blotting and reverse-transcription PCR analysis showed that the 1.0 kb mRNA was present at higher levels than the 1.6 kb species in immature cells including HL-60 and bone-marrow cells.
T13 1907-2007 Sentence denotes By contrast, low levels of 1.6 kb mRNA transcripts predominated in differentiated blood eosinophils.
T14 2008-2157 Sentence denotes The results are compatible with differential use of P1 and P2 promoters as a mechanism for regulation of MBP expression during eosinophil maturation.
T1 0-131 Sentence denotes Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters.
T2 132-269 Sentence denotes Human eosinophil major basic protein (MBP) is one of the principal mediators of injury to parasites and tissues in allergic inflammation.
T3 270-389 Sentence denotes MBP is stored in eosinophil crystalloid granules and released with other granule constituents during eosinophil action.
T4 390-565 Sentence denotes Previous studies have identified an MBP gene promoter that generates a 1.0 kb mRNA transcript encoding MBP preproprotein which undergoes processing to the mature storage form.
T5 566-725 Sentence denotes To investigate how the MBP gene is regulated, we have examined the identity and levels of the MBP transcripts both in precursor cells and in blood eosinophils.
T6 726-869 Sentence denotes It was found that the gene was expressed from two upstream promoters, a distal promoter P1 in addition to the previously described promoter P2.
T7 870-1058 Sentence denotes Evidence for the second promoter was initially provided by isolation from a human HL-60 leukaemic cell cDNA library of a novel 1.6 kb MBP cDNA that was distinct from the known 1.0 kb cDNA.
T8 1059-1241 Sentence denotes The complete nucleotide sequence of the 1.6 kb cDNA was determined, and showed that the two cDNAs had identical coding and 3' untranslated regions but differed in their 5' sequences.
T9 1242-1423 Sentence denotes By isolating and sequencing MBP genomic clones from an arrayed chromosome 11 library, it was demonstrated that the MBP gene is composed of nine upstream exons and five coding exons.
T10 1424-1587 Sentence denotes The 1.6 and 1.0 kb cDNAs arise by differential splicing of alternate MBP transcripts from promoters P1 and P2 respectively, located 32 kb apart in the genomic DNA.
T11 1588-1711 Sentence denotes Primer extension analysis identified two transcription start sites at P1, neither associated with a typical TATA box motif.
T12 1712-1906 Sentence denotes Northern blotting and reverse-transcription PCR analysis showed that the 1.0 kb mRNA was present at higher levels than the 1.6 kb species in immature cells including HL-60 and bone-marrow cells.
T13 1907-2007 Sentence denotes By contrast, low levels of 1.6 kb mRNA transcripts predominated in differentiated blood eosinophils.
T14 2008-2157 Sentence denotes The results are compatible with differential use of P1 and P2 promoters as a mechanism for regulation of MBP expression during eosinophil maturation.