> top > docs > PubMed:7499266 > annotations

PubMed:7499266 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-160 Sentence denotes Identification of an I kappa B alpha-associated protein kinase in a human monocytic cell line and determination of its phosphorylation sites on I kappa B alpha.
T2 161-279 Sentence denotes Nuclear factor kappa B (NF-kappa B) is stored in the cytoplasm as an inactive form through interaction with I kappa B.
T3 280-419 Sentence denotes Stimulation of cells leads to a rapid phosphorylation of I kappa B alpha, which is presumed to be important for the subsequent degradation.
T4 420-615 Sentence denotes We have recently reported the establishment of a lipopolysaccharide (LPS)-dependent cell-free activation system of NF-kappa B in association with the induction of I kappa B alpha phosphorylation.
T5 616-793 Sentence denotes In this study, we have identified a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha.
T6 794-888 Sentence denotes LPS stimulation transiently enhanced the I kappa B alpha-bound kinase activity in THP-1 cells.
T7 889-1118 Sentence denotes Mutational analyses of I kappa B alpha and competition experiments with the synthetic peptides identified major phosphorylation sites by the bound kinase as Ser and Thr residues in the C-terminal acidic domain of I kappa B alpha.
T8 1119-1374 Sentence denotes Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS-induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells.
T9 1375-1611 Sentence denotes These results suggested that the bound kinase is involved in the signaling pathway of LPS by inducing the phosphorylation of the C-terminal region of I kappa B alpha and subsequent dissociation of the NF-kappa B.I kappa B alpha complex.
T1 0-160 Sentence denotes Identification of an I kappa B alpha-associated protein kinase in a human monocytic cell line and determination of its phosphorylation sites on I kappa B alpha.
T2 161-279 Sentence denotes Nuclear factor kappa B (NF-kappa B) is stored in the cytoplasm as an inactive form through interaction with I kappa B.
T3 280-419 Sentence denotes Stimulation of cells leads to a rapid phosphorylation of I kappa B alpha, which is presumed to be important for the subsequent degradation.
T4 420-615 Sentence denotes We have recently reported the establishment of a lipopolysaccharide (LPS)-dependent cell-free activation system of NF-kappa B in association with the induction of I kappa B alpha phosphorylation.
T5 616-793 Sentence denotes In this study, we have identified a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha.
T6 794-888 Sentence denotes LPS stimulation transiently enhanced the I kappa B alpha-bound kinase activity in THP-1 cells.
T7 889-1118 Sentence denotes Mutational analyses of I kappa B alpha and competition experiments with the synthetic peptides identified major phosphorylation sites by the bound kinase as Ser and Thr residues in the C-terminal acidic domain of I kappa B alpha.
T8 1119-1374 Sentence denotes Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS-induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells.
T9 1375-1611 Sentence denotes These results suggested that the bound kinase is involved in the signaling pathway of LPS by inducing the phosphorylation of the C-terminal region of I kappa B alpha and subsequent dissociation of the NF-kappa B.I kappa B alpha complex.

jnlpba-st-training

Id Subject Object Predicate Lexical cue
T1 21-62 protein denotes I kappa B alpha-associated protein kinase
T2 68-93 cell_line denotes human monocytic cell line
T3 119-140 protein denotes phosphorylation sites
T4 144-159 protein denotes I kappa B alpha
T5 161-183 protein denotes Nuclear factor kappa B
T6 185-195 protein denotes NF-kappa B
T7 269-278 protein denotes I kappa B
T8 337-352 protein denotes I kappa B alpha
T9 535-545 protein denotes NF-kappa B
T10 583-598 protein denotes I kappa B alpha
T11 652-658 protein denotes kinase
T12 685-725 cell_line denotes LPS-stimulated human monocytic cell line
T13 727-732 cell_line denotes THP-1
T14 777-792 protein denotes I kappa B alpha
T15 835-850 protein denotes I kappa B alpha
T16 850-863 protein denotes -bound kinase
T17 876-887 cell_line denotes THP-1 cells
T18 912-921 protein denotes I kappa B
T19 1001-1022 protein denotes phosphorylation sites
T20 1036-1042 protein denotes kinase
T21 1074-1098 protein denotes C-terminal acidic domain
T22 1102-1117 protein denotes I kappa B alpha
T23 1176-1200 protein denotes C-terminal acidic domain
T24 1204-1219 protein denotes I kappa B alpha
T25 1245-1255 protein denotes NF-kappa B
T26 1318-1333 protein denotes I kappa B alpha
T27 1362-1373 cell_line denotes THP-1 cells
T28 1408-1420 protein denotes bound kinase
T29 1504-1521 protein denotes C-terminal region
T30 1525-1540 protein denotes I kappa B alpha
T31 1576-1586 protein denotes NF-kappa B
T32 1587-1610 protein denotes I kappa B alpha complex

genia-medco-coref

Id Subject Object Predicate Lexical cue
C1 18-62 NP denotes an I kappa B alpha-associated protein kinase
C2 66-93 NP denotes a human monocytic cell line
C4 115-118 NP denotes its
C5 144-159 NP denotes I kappa B alpha
C3 115-159 NP denotes its phosphorylation sites on I kappa B alpha
C6 161-196 NP denotes Nuclear factor kappa B (NF-kappa B)
C8 337-352 NP denotes I kappa B alpha
C7 310-352 NP denotes a rapid phosphorylation of I kappa B alpha
C9 354-359 NP denotes which
C10 535-545 NP denotes NF-kappa B
C12 650-658 NP denotes a kinase
C13 681-725 NP denotes the LPS-stimulated human monocytic cell line
C14 727-732 NP denotes THP-1
C15 734-738 NP denotes that
C16 777-792 NP denotes I kappa B alpha
C11 650-792 NP denotes a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha
C17 876-887 NP denotes THP-1 cells
C18 912-927 NP denotes I kappa B alpha
C20 1026-1042 NP denotes the bound kinase
C19 995-1042 NP denotes major phosphorylation sites by the bound kinase
C22 1102-1117 NP denotes I kappa B alpha
C21 1070-1117 NP denotes the C-terminal acidic domain of I kappa B alpha
C24 1204-1219 NP denotes I kappa B alpha
C23 1172-1219 NP denotes the C-terminal acidic domain of I kappa B alpha
C25 1362-1373 NP denotes THP-1 cells
C26 1404-1420 NP denotes the bound kinase
C28 1525-1540 NP denotes I kappa B alpha
C27 1500-1540 NP denotes the C-terminal region of I kappa B alpha
R1 C4 C1 coref-pron its,an I kappa B alpha-associated protein kinase
R2 C8 C5 coref-ident I kappa B alpha,I kappa B alpha
R3 C9 C7 coref-relat which,a rapid phosphorylation of I kappa B alpha
R4 C10 C6 coref-ident NF-kappa B,Nuclear factor kappa B (NF-kappa B)
R5 C13 C2 coref-ident the LPS-stimulated human monocytic cell line,a human monocytic cell line
R6 C14 C13 coref-appos THP-1,the LPS-stimulated human monocytic cell line
R7 C15 C12 coref-relat that,a kinase
R8 C16 C8 coref-ident I kappa B alpha,I kappa B alpha
R9 C11 C1 coref-ident "a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha",an I kappa B alpha-associated protein kinase
R10 C17 C13 coref-ident THP-1 cells,the LPS-stimulated human monocytic cell line
R11 C18 C16 coref-ident I kappa B alpha,I kappa B alpha
R12 C19 C3 coref-ident major phosphorylation sites by the bound kinase,its phosphorylation sites on I kappa B alpha
R13 C22 C18 coref-ident I kappa B alpha,I kappa B alpha
R14 C24 C22 coref-ident I kappa B alpha,I kappa B alpha
R15 C23 C21 coref-ident the C-terminal acidic domain of I kappa B alpha,the C-terminal acidic domain of I kappa B alpha
R16 C25 C17 coref-ident THP-1 cells,THP-1 cells
R17 C26 C20 coref-ident the bound kinase,the bound kinase
R18 C28 C24 coref-ident I kappa B alpha,I kappa B alpha
R19 C27 C23 coref-ident the C-terminal region of I kappa B alpha,the C-terminal acidic domain of I kappa B alpha

pubmed-sentences-benchmark

Id Subject Object Predicate Lexical cue
S1 0-160 Sentence denotes Identification of an I kappa B alpha-associated protein kinase in a human monocytic cell line and determination of its phosphorylation sites on I kappa B alpha.
S2 161-279 Sentence denotes Nuclear factor kappa B (NF-kappa B) is stored in the cytoplasm as an inactive form through interaction with I kappa B.
S3 280-419 Sentence denotes Stimulation of cells leads to a rapid phosphorylation of I kappa B alpha, which is presumed to be important for the subsequent degradation.
S4 420-615 Sentence denotes We have recently reported the establishment of a lipopolysaccharide (LPS)-dependent cell-free activation system of NF-kappa B in association with the induction of I kappa B alpha phosphorylation.
S5 616-793 Sentence denotes In this study, we have identified a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha.
S6 794-888 Sentence denotes LPS stimulation transiently enhanced the I kappa B alpha-bound kinase activity in THP-1 cells.
S7 889-1118 Sentence denotes Mutational analyses of I kappa B alpha and competition experiments with the synthetic peptides identified major phosphorylation sites by the bound kinase as Ser and Thr residues in the C-terminal acidic domain of I kappa B alpha.
S8 1119-1374 Sentence denotes Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS-induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells.
S9 1375-1611 Sentence denotes These results suggested that the bound kinase is involved in the signaling pathway of LPS by inducing the phosphorylation of the C-terminal region of I kappa B alpha and subsequent dissociation of the NF-kappa B.I kappa B alpha complex.

GENIAcorpus

Id Subject Object Predicate Lexical cue
T1 21-62 protein_molecule denotes I kappa B alpha-associated protein kinase
T2 68-93 cell_line denotes human monocytic cell line
T3 119-140 protein_domain_or_region denotes phosphorylation sites
T4 144-159 protein_molecule denotes I kappa B alpha
T5 161-183 protein_molecule denotes Nuclear factor kappa B
T6 185-195 protein_molecule denotes NF-kappa B
T7 214-223 cell_component denotes cytoplasm
T8 269-278 protein_molecule denotes I kappa B
T9 312-333 other_name denotes rapid phosphorylation
T10 337-346 protein_molecule denotes I kappa B
T11 396-418 other_name denotes subsequent degradation
T12 469-487 lipid denotes lipopolysaccharide
T13 489-492 lipid denotes LPS
T14 535-545 protein_molecule denotes NF-kappa B
T15 583-592 protein_molecule denotes I kappa B
T16 652-658 protein_family_or_group denotes kinase
T17 662-675 cell_component denotes cell extracts
T18 685-688 lipid denotes LPS
T19 727-732 cell_line denotes THP-1
T20 777-792 protein_molecule denotes I kappa B alpha
T21 794-797 lipid denotes LPS
T22 835-844 protein_molecule denotes I kappa B
T23 850-863 protein_family_or_group denotes -bound kinase
T24 876-887 cell_line denotes THP-1 cells
T25 912-921 protein_molecule denotes I kappa B
T26 932-955 other_name denotes competition experiments
T27 965-983 peptide denotes synthetic peptides
T28 1001-1022 protein_domain_or_region denotes phosphorylation sites
T29 1036-1042 protein_molecule denotes kinase
T30 1046-1049 amino_acid_monomer denotes Ser
T31 1054-1057 amino_acid_monomer denotes Thr
T32 1074-1098 protein_domain_or_region denotes C-terminal acidic domain
T33 1102-1111 protein_molecule denotes I kappa B
T34 1146-1153 peptide denotes peptide
T35 1176-1200 protein_domain_or_region denotes C-terminal acidic domain
T36 1204-1213 protein_molecule denotes I kappa B
T37 1233-1236 lipid denotes LPS
T38 1245-1255 protein_molecule denotes NF-kappa B
T39 1288-1303 other_name denotes phosphorylation
T40 1318-1327 protein_molecule denotes I kappa B
T41 1339-1355 other_name denotes cell-free system
T42 1362-1373 cell_line denotes THP-1 cells
T43 1408-1420 protein_family_or_group denotes bound kinase
T44 1461-1464 lipid denotes LPS
T45 1481-1496 other_name denotes phosphorylation
T46 1504-1521 protein_domain_or_region denotes C-terminal region
T47 1525-1534 protein_molecule denotes I kappa B
T48 1576-1586 protein_molecule denotes NF-kappa B
T49 1587-1602 protein_molecule denotes I kappa B alpha

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 68-73 OrganismTaxon denotes human 9606
T2 700-705 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 214-223 Body_part denotes cytoplasm http://purl.obolibrary.org/obo/GO_0005737

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 74-83 Cell denotes monocytic http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T3 706-715 Cell denotes monocytic http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054