PubMed:721898 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/721898","sourcedb":"PubMed","sourceid":"721898","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/721898","text":"Localization of intestinal sucrase-isomaltase complex on the microvillous membrane by electron microscopy using nonlabeled antibodies.\nMicrovillous vesicles isolated from rabbit small intestine showed a trilaminar membrane with a rather smooth surface, which was apparently not affected by papain solubilizing sucrase-isomaltase complex or by trypsin unable to solubilize it. When microvilous vesicles or trysinized ones were incubated with immunoglobulin G against the sucrase-isomaltase complex or monovalent fragments therefrom, an apparently continuous electron-opaque layer approximately 180 A in width appeared around the external surface of vesicles. Such a layer was not formed on papainized vesicles. Microvillous vesicles and trypsinized ones negatively stained with phosphotungate showed a great number of particles protruding approximately 150 A from the membrane surface, but papainized vesicles did not. The particles existed close to one another and appeared to form a particulate layer 150 A in width on the surface. The antibodies, whether they were divalent or monovalent, increased the width of the layer to approximately 200 A and obscured the fine particulate structure of intact and trypsinized vesicles. Papainized vesicles retained their smooth surface upon interaction with antibodies. These results, together with those with the Triton-solubilized sucrase- isomaltase complex (Nishi and Takesue, 1978), J. Ultra-struct. Res., 62:1- 12), indicate not only that sucrase-isomaltase complexes are located close to one another on the membrane, but also that they or at least their protein portions protrude approximately 150 A from the surface of the trilaminar membrane.","tracks":[{"project":"NEUROSES","denotations":[{"id":"T1","span":{"begin":46,"end":53},"obj":"PATO_0001504"},{"id":"T2","span":{"begin":329,"end":336},"obj":"PATO_0001504"},{"id":"T3","span":{"begin":489,"end":496},"obj":"PATO_0001504"},{"id":"T4","span":{"begin":1394,"end":1401},"obj":"PATO_0001504"},{"id":"T5","span":{"begin":86,"end":94},"obj":"CHEBI_10545"},{"id":"T6","span":{"begin":557,"end":565},"obj":"CHEBI_10545"},{"id":"T7","span":{"begin":178,"end":183},"obj":"PATO_0000587"},{"id":"T8","span":{"begin":237,"end":243},"obj":"PATO_0000701"},{"id":"T9","span":{"begin":1262,"end":1268},"obj":"PATO_0000701"},{"id":"T10","span":{"begin":343,"end":350},"obj":"CHEBI_9765"},{"id":"T11","span":{"begin":546,"end":556},"obj":"PATO_0000689"},{"id":"T12","span":{"begin":566,"end":572},"obj":"PATO_0000963"},{"id":"T13","span":{"begin":602,"end":607},"obj":"PATO_0000921"},{"id":"T14","span":{"begin":1011,"end":1016},"obj":"PATO_0000921"},{"id":"T15","span":{"begin":1105,"end":1110},"obj":"PATO_0000921"},{"id":"T16","span":{"begin":801,"end":806},"obj":"PATO_0000586"},{"id":"T19","span":{"begin":909,"end":912},"obj":"CHEBI_52027"},{"id":"T22","span":{"begin":1091,"end":1100},"obj":"PATO_0000470"},{"id":"T23","span":{"begin":1181,"end":1190},"obj":"PATO_0000141"},{"id":"T24","span":{"begin":1355,"end":1361},"obj":"CHEBI_29234"},{"id":"T25","span":{"begin":1602,"end":1609},"obj":"CHEBI_16541"},{"id":"T26","span":{"begin":1602,"end":1609},"obj":"CHEBI_36080"}],"attributes":[{"subj":"T1","pred":"source","obj":"NEUROSES"},{"subj":"T2","pred":"source","obj":"NEUROSES"},{"subj":"T3","pred":"source","obj":"NEUROSES"},{"subj":"T4","pred":"source","obj":"NEUROSES"},{"subj":"T5","pred":"source","obj":"NEUROSES"},{"subj":"T6","pred":"source","obj":"NEUROSES"},{"subj":"T7","pred":"source","obj":"NEUROSES"},{"subj":"T8","pred":"source","obj":"NEUROSES"},{"subj":"T9","pred":"source","obj":"NEUROSES"},{"subj":"T10","pred":"source","obj":"NEUROSES"},{"subj":"T11","pred":"source","obj":"NEUROSES"},{"subj":"T12","pred":"source","obj":"NEUROSES"},{"subj":"T13","pred":"source","obj":"NEUROSES"},{"subj":"T14","pred":"source","obj":"NEUROSES"},{"subj":"T15","pred":"source","obj":"NEUROSES"},{"subj":"T16","pred":"source","obj":"NEUROSES"},{"subj":"T19","pred":"source","obj":"NEUROSES"},{"subj":"T22","pred":"source","obj":"NEUROSES"},{"subj":"T23","pred":"source","obj":"NEUROSES"},{"subj":"T24","pred":"source","obj":"NEUROSES"},{"subj":"T25","pred":"source","obj":"NEUROSES"},{"subj":"T26","pred":"source","obj":"NEUROSES"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NEUROSES","color":"#93b3ec","default":true}]}]}}