PubMed:7107573 JSON TXT

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Test-Species-PubTator

{"project":"Test-Species-PubTator","denotations":[{"id":"1","span":{"begin":63,"end":86},"obj":"Species"},{"id":"28","span":{"begin":190,"end":213},"obj":"Species"},{"id":"52","span":{"begin":1687,"end":1692},"obj":"Species"},{"id":"53","span":{"begin":1709,"end":1714},"obj":"Species"}],"attributes":[{"id":"A1","pred":"resolved_to","subj":"1","obj":"3850"},{"id":"A28","pred":"resolved_to","subj":"28","obj":"3850"},{"id":"A52","pred":"resolved_to","subj":"52","obj":"10090"},{"id":"A53","pred":"resolved_to","subj":"53","obj":"9606"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Test-Species-PubDictionaries

{"project":"Test-Species-PubDictionaries","denotations":[{"id":"T1","span":{"begin":63,"end":86},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":190,"end":213},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":840,"end":846},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1687,"end":1692},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":1709,"end":1714},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"3850"},{"id":"A2","pred":"db_id","subj":"T2","obj":"3850"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9913"},{"id":"A4","pred":"db_id","subj":"T4","obj":"10088"},{"id":"A5","pred":"db_id","subj":"T4","obj":"10090"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9606"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Test-Species-PubDictionaries-PubMedBERT

{"project":"Test-Species-PubDictionaries-PubMedBERT","denotations":[{"id":"T1","span":{"begin":63,"end":86},"obj":"Species"},{"id":"T2","span":{"begin":87,"end":92},"obj":"Species"},{"id":"T3","span":{"begin":144,"end":147},"obj":"Species"},{"id":"T4","span":{"begin":190,"end":213},"obj":"Species"},{"id":"T5","span":{"begin":214,"end":219},"obj":"Species"},{"id":"T6","span":{"begin":253,"end":256},"obj":"Species"},{"id":"T7","span":{"begin":546,"end":552},"obj":"Species"},{"id":"T8","span":{"begin":821,"end":826},"obj":"Species"},{"id":"T9","span":{"begin":827,"end":830},"obj":"Species"},{"id":"T10","span":{"begin":840,"end":846},"obj":"Species"},{"id":"T11","span":{"begin":1687,"end":1692},"obj":"Species"},{"id":"T13","span":{"begin":1709,"end":1714},"obj":"Species"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"3850"},{"id":"A2","pred":"db_id","subj":"T2","obj":"58024"},{"id":"A3","pred":"db_id","subj":"T3","obj":"98090"},{"id":"A4","pred":"db_id","subj":"T4","obj":"3850"},{"id":"A5","pred":"db_id","subj":"T5","obj":"58024"},{"id":"A6","pred":"db_id","subj":"T6","obj":"98090"},{"id":"A7","pred":"db_id","subj":"T7","obj":"48766"},{"id":"A8","pred":"db_id","subj":"T8","obj":"536441"},{"id":"A9","pred":"db_id","subj":"T9","obj":"98090"},{"id":"A10","pred":"db_id","subj":"T10","obj":"9913"},{"id":"A11","pred":"db_id","subj":"T11","obj":"10088"},{"id":"A12","pred":"db_id","subj":"T11","obj":"10090"},{"id":"A13","pred":"db_id","subj":"T13","obj":"9606"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos6-Glycan-Motif-Image

sentences

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":440},"obj":"Sentence"},{"id":"T3","span":{"begin":441,"end":586},"obj":"Sentence"},{"id":"T4","span":{"begin":587,"end":772},"obj":"Sentence"},{"id":"T5","span":{"begin":773,"end":1062},"obj":"Sentence"},{"id":"T6","span":{"begin":1063,"end":1301},"obj":"Sentence"},{"id":"T7","span":{"begin":1302,"end":1524},"obj":"Sentence"},{"id":"T8","span":{"begin":1525,"end":1638},"obj":"Sentence"},{"id":"T9","span":{"begin":1639,"end":1738},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":440},"obj":"Sentence"},{"id":"T3","span":{"begin":441,"end":586},"obj":"Sentence"},{"id":"T4","span":{"begin":587,"end":772},"obj":"Sentence"},{"id":"T5","span":{"begin":773,"end":1062},"obj":"Sentence"},{"id":"T6","span":{"begin":1063,"end":1301},"obj":"Sentence"},{"id":"T7","span":{"begin":1302,"end":1524},"obj":"Sentence"},{"id":"T8","span":{"begin":1525,"end":1638},"obj":"Sentence"},{"id":"T9","span":{"begin":1639,"end":1738},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos6-Glycan-Motif-Structure

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":34,"end":43},"obj":"https://glytoucan.org/Structures/Glycans/G48600JD"},{"id":"T2","span":{"begin":36,"end":43},"obj":"https://glytoucan.org/Structures/Glycans/G00048MO"},{"id":"T3","span":{"begin":36,"end":43},"obj":"https://glytoucan.org/Structures/Glycans/G39211DH"},{"id":"T4","span":{"begin":135,"end":148},"obj":"https://glytoucan.org/Structures/Glycans/G00048MO"},{"id":"T5","span":{"begin":135,"end":148},"obj":"https://glytoucan.org/Structures/Glycans/G39211DH"},{"id":"T6","span":{"begin":253,"end":256},"obj":"https://glytoucan.org/Structures/Glycans/G39211DH"},{"id":"T7","span":{"begin":266,"end":272},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T8","span":{"begin":323,"end":329},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T9","span":{"begin":613,"end":620},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T10","span":{"begin":622,"end":641},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"},{"id":"T11","span":{"begin":643,"end":649},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T12","span":{"begin":827,"end":830},"obj":"https://glytoucan.org/Structures/Glycans/G39211DH"},{"id":"T13","span":{"begin":898,"end":904},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T14","span":{"begin":955,"end":961},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T15","span":{"begin":1031,"end":1038},"obj":"https://glytoucan.org/Structures/Glycans/G00047MO"},{"id":"T16","span":{"begin":1031,"end":1038},"obj":"https://glytoucan.org/Structures/Glycans/G39023AU"},{"id":"T17","span":{"begin":1527,"end":1533},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Glycosmos6-GlycoEpitope

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":36,"end":43},"obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"T2","span":{"begin":135,"end":142},"obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"T3","span":{"begin":144,"end":147},"obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"T4","span":{"begin":253,"end":256},"obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"T5","span":{"begin":827,"end":830},"obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"T6","span":{"begin":1031,"end":1038},"obj":"http://www.glycoepitope.jp/epitopes/EP0007"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Glycosmos6-MAT

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":815,"end":820},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T2","span":{"begin":815,"end":820},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T3","span":{"begin":1693,"end":1699},"obj":"http://purl.obolibrary.org/obo/MAT_0000085"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

NGLY1-deficiency

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":353,"end":359},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":622,"end":641},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T3","span":{"begin":985,"end":991},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T4","span":{"begin":1289,"end":1295},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T5","span":{"begin":1353,"end":1359},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Anatomy-MAT

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":815,"end":820},"obj":"Body_part"},{"id":"T3","span":{"begin":1693,"end":1699},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A2","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000085"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-Glycan

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":36,"end":43},"obj":"Glycan"},{"id":"T2","span":{"begin":135,"end":142},"obj":"Glycan"},{"id":"T3","span":{"begin":144,"end":147},"obj":"Glycan"},{"id":"T4","span":{"begin":253,"end":256},"obj":"Glycan"},{"id":"T5","span":{"begin":827,"end":830},"obj":"Glycan"},{"id":"T6","span":{"begin":1031,"end":1038},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A7","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A8","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A9","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A10","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A11","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G39023AU"},{"id":"A12","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39023AU"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Glycan-GlyCosmos

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":36,"end":43},"obj":"Glycan"},{"id":"T2","span":{"begin":135,"end":142},"obj":"Glycan"},{"id":"T3","span":{"begin":144,"end":147},"obj":"Glycan"},{"id":"T4","span":{"begin":253,"end":256},"obj":"Glycan"},{"id":"T5","span":{"begin":827,"end":830},"obj":"Glycan"},{"id":"T6","span":{"begin":1031,"end":1038},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A7","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A8","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A9","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A10","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G00048MO"},{"id":"A11","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00048MO"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G39023AU"},{"id":"A12","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39023AU"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos-GlycoEpitope

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":36,"end":43},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":135,"end":142},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":144,"end":147},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":253,"end":256},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":827,"end":830},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":1031,"end":1038},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0007"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-CL

{"project":"GlyCosmos15-CL","denotations":[{"id":"T1","span":{"begin":1726,"end":1737},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000542"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-UBERON

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":815,"end":820},"obj":"Body_part"},{"id":"T2","span":{"begin":1693,"end":1699},"obj":"Body_part"},{"id":"T3","span":{"begin":1726,"end":1737},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000542"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-Taxon

{"project":"GlyCosmos15-Taxon","denotations":[{"id":"T1","span":{"begin":63,"end":86},"obj":"Organism"},{"id":"T2","span":{"begin":190,"end":213},"obj":"Organism"},{"id":"T3","span":{"begin":840,"end":846},"obj":"Organism"},{"id":"T4","span":{"begin":1687,"end":1692},"obj":"Organism"},{"id":"T6","span":{"begin":1709,"end":1714},"obj":"Organism"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"3850"},{"id":"A2","pred":"db_id","subj":"T2","obj":"3850"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9913"},{"id":"A4","pred":"db_id","subj":"T4","obj":"10088"},{"id":"A5","pred":"db_id","subj":"T4","obj":"10090"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9606"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-GlycoEpitope

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":36,"end":43},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":135,"end":142},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":144,"end":147},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":253,"end":256},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":827,"end":830},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":1031,"end":1038},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0010"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0007"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-Sentences

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":440},"obj":"Sentence"},{"id":"T3","span":{"begin":441,"end":586},"obj":"Sentence"},{"id":"T4","span":{"begin":587,"end":772},"obj":"Sentence"},{"id":"T5","span":{"begin":773,"end":1062},"obj":"Sentence"},{"id":"T6","span":{"begin":1063,"end":1301},"obj":"Sentence"},{"id":"T7","span":{"begin":1302,"end":1524},"obj":"Sentence"},{"id":"T8","span":{"begin":1525,"end":1638},"obj":"Sentence"},{"id":"T9","span":{"begin":1639,"end":1738},"obj":"Sentence"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Lectin-Jamboree-Sentence

{"project":"Lectin-Jamboree-Sentence","blocks":[{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":440},"obj":"Sentence"},{"id":"T3","span":{"begin":441,"end":586},"obj":"Sentence"},{"id":"T4","span":{"begin":587,"end":772},"obj":"Sentence"},{"id":"T5","span":{"begin":773,"end":1062},"obj":"Sentence"},{"id":"T6","span":{"begin":1063,"end":1301},"obj":"Sentence"},{"id":"T7","span":{"begin":1302,"end":1524},"obj":"Sentence"},{"id":"T8","span":{"begin":1525,"end":1638},"obj":"Sentence"},{"id":"T9","span":{"begin":1639,"end":1738},"obj":"Sentence"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-FMA

{"project":"GlyCosmos15-FMA","denotations":[{"id":"T1","span":{"begin":815,"end":820},"obj":"Body_part"},{"id":"T2","span":{"begin":847,"end":852},"obj":"Body_part"},{"id":"T3","span":{"begin":1693,"end":1699},"obj":"Body_part"},{"id":"T4","span":{"begin":1726,"end":1737},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"FMA:9670"},{"id":"A2","pred":"db_id","subj":"T2","obj":"FMA:63083"},{"id":"A3","pred":"db_id","subj":"T3","obj":"FMA:7196"},{"id":"A4","pred":"db_id","subj":"T4","obj":"FMA:62863"}],"namespaces":[{"prefix":"FMA","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

GlyCosmos15-MAT

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":815,"end":820},"obj":"Body_part"},{"id":"T2","span":{"begin":1693,"end":1699},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000085"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

NCBITAXON

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":63,"end":86},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":190,"end":213},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":840,"end":846},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1687,"end":1692},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":1709,"end":1714},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"3850"},{"id":"A2","pred":"db_id","subj":"T2","obj":"3850"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9913"},{"id":"A4","pred":"db_id","subj":"T4","obj":"10088"},{"id":"A5","pred":"db_id","subj":"T4","obj":"10090"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9606"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

Anatomy-UBERON

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":815,"end":820},"obj":"Body_part"},{"id":"T2","span":{"begin":1693,"end":1699},"obj":"Body_part"},{"id":"T3","span":{"begin":1726,"end":1737},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000542"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}

CL-cell

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":1726,"end":1737},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000542"}],"text":"Isolation and characterization of a Lewis b-active lectin from Griffonia simplicifolia seeds.\nA fourth lectin (GS IV), a serologically Lewis b (Leb)-active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 3)-[alpha-L-fucose-(1 leads to 4)]-beta-D-GlcNAc)-active fragment coupled to Synsorb, followed by elution with 0.1 N acetic acid. The purified lectin was shown to be homogeneous by polyacrylamide gel electrophoresis and analytical gel column chromatography on Sephadex G-200. A glycoprotein containing mannose, N-acetylglucosamine, fucose, and xylose (4:2:1:1), the lectin is a dimer composed of subunits of Mr = 29,000 and 27,000 with an aggregate Mr = 56,000. The GS IV lectin precipitate equally with blood group Leb fragment-bovine serum albumin (BSA) and H-active fragment (alpha-L-fucose-(1 leads to 2)-beta-D-Gal-(1 leads to 4)-[alpha-L-fucose-(1 leads to 3)]-beta-D-GlcNAc)-BSA conjugates but only poorly with a Lewis a fragment-BSA conjugate. Inhibition of precipitation with various mono- and oligosaccharides indicated that the lectin is specific for type 1 or 2 chains containing two alpha-L-fucosyl groups on C-2 of the D-galactosyl residue and C-3 or C-4 of the D-GlcNAc unit. One alpha-L-fucosyl residue on C-3 or C-4 of the D-GlcNAc residue of type 1 or 2 chains inhibited the reaction, although these oligosaccharides were less active than the oligosaccharides containing 2 alpha-L-fucosyl units. L-Fucose, methyl alpha- or beta-fucoside, and p-nitrophenyl alpha- or beta-L-fucoside did not inhibit the lectin. GSIV did not display mitogenic activity against mouse spleen cells or human peripheral lymphocytes."}