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sentences

Id Subject Object Predicate Lexical cue
T1 0-156 Sentence denotes Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli.
T2 157-343 Sentence denotes Only one of the sulfhydryl groups from Cys-32 and Cys-35 in the active center of native Escherichia coli thioredoxin-(SH)2 was alkylated by excess iodoacetic acid at pH values below 8.0.
T3 344-424 Sentence denotes Both groups reacted in the protein denatured with 4.5 M guanidine hydrochloride.
T4 425-654 Sentence denotes The second order rate of alkylation of thioredoxin-(SH)2 with 1 eq of iodoacetic acid was pH-dependent and showed independent initial reactions of one thiolate ion with a pK value of 6.7 and a second with a pK value close to 9.0.
T5 655-850 Sentence denotes The same pH dependence was observed for alkylation with iodoacetamide but the apparent rate constant, 107 M-1 S-1 at pH 7.2, was about 20-fold higher than the corresponding rate with iodoacetate.
T6 851-1096 Sentence denotes The sulfhydryl group with a pK value of 6.7 was shown to belong to Cys-32 by labeling thioredoxin with [14C]iodoacetic acid followed by complete alkylation with [3H]iodoacetate and amino acid sequence analysis of peptides from the active center.
T7 1097-1236 Sentence denotes The abnormally low pK value of Cys-32 is suggested to arise by electrostatic influence from a positive charge on the amino group of Lys-36.
T8 1237-1334 Sentence denotes A mechanism of action for thioredoxin-(SH)2 as a protein disulfide reductase has been formulated.
T9 1335-1526 Sentence denotes This is based on an initial nucleophilic attack by the thiolate of Cys-32 with the formation of an unstable transient mixed disulfide involving Cys-32 and one of the sulfurs in the substrate.
T10 1527-1697 Sentence denotes This is followed by a conformational change and a nucleophilic attack of Cys-35 to give the 14-membered disulfide ring in thioredoxin-S2 and the dithiol of the substrate.
T1 0-156 Sentence denotes Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli.
T2 157-343 Sentence denotes Only one of the sulfhydryl groups from Cys-32 and Cys-35 in the active center of native Escherichia coli thioredoxin-(SH)2 was alkylated by excess iodoacetic acid at pH values below 8.0.
T3 344-424 Sentence denotes Both groups reacted in the protein denatured with 4.5 M guanidine hydrochloride.
T4 425-654 Sentence denotes The second order rate of alkylation of thioredoxin-(SH)2 with 1 eq of iodoacetic acid was pH-dependent and showed independent initial reactions of one thiolate ion with a pK value of 6.7 and a second with a pK value close to 9.0.
T5 655-850 Sentence denotes The same pH dependence was observed for alkylation with iodoacetamide but the apparent rate constant, 107 M-1 S-1 at pH 7.2, was about 20-fold higher than the corresponding rate with iodoacetate.
T6 851-1096 Sentence denotes The sulfhydryl group with a pK value of 6.7 was shown to belong to Cys-32 by labeling thioredoxin with [14C]iodoacetic acid followed by complete alkylation with [3H]iodoacetate and amino acid sequence analysis of peptides from the active center.
T7 1097-1236 Sentence denotes The abnormally low pK value of Cys-32 is suggested to arise by electrostatic influence from a positive charge on the amino group of Lys-36.
T8 1237-1334 Sentence denotes A mechanism of action for thioredoxin-(SH)2 as a protein disulfide reductase has been formulated.
T9 1335-1526 Sentence denotes This is based on an initial nucleophilic attack by the thiolate of Cys-32 with the formation of an unstable transient mixed disulfide involving Cys-32 and one of the sulfurs in the substrate.
T10 1527-1697 Sentence denotes This is followed by a conformational change and a nucleophilic attack of Cys-35 to give the 14-membered disulfide ring in thioredoxin-S2 and the dithiol of the substrate.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 139-155 OrganismTaxon denotes Escherichia coli 562
T2 245-261 OrganismTaxon denotes Escherichia coli 562