PubMed:6520117 JSONTXT

{"project":"silkworm_phenotype","denotations":[{"id":"T15","span":{"begin":873,"end":880},"obj":"BMO_00547"},{"id":"T14","span":{"begin":653,"end":660},"obj":"BMO_00547"},{"id":"T12","span":{"begin":632,"end":651},"obj":"BMO_00547"},{"id":"T11","span":{"begin":828,"end":843},"obj":"BMO_00548"},{"id":"T8","span":{"begin":1125,"end":1137},"obj":"Chemical:MESH:D017830"},{"id":"T7","span":{"begin":884,"end":893},"obj":"Chemical:MESH:D004220"},{"id":"T6","span":{"begin":828,"end":843},"obj":"Gene:693047"},{"id":"T5","span":{"begin":632,"end":651},"obj":"Gene:693030"},{"id":"T4","span":{"begin":243,"end":254},"obj":"Species:7091"},{"id":"T3","span":{"begin":233,"end":241},"obj":"Species:7091"},{"id":"T2","span":{"begin":144,"end":155},"obj":"Species:7091"},{"id":"T1","span":{"begin":134,"end":142},"obj":"Species:7091"}],"text":"Predominant synthesis of fibroin heavy and light chains on the membrane-bound polysomes prepared from the posterior silk gland of the silkworm, Bombyx mori.\nMembrane-bound polysomes were prepared from the posterior silk gland of the silkworm, Bombyx mori, on the fourth to fifth day in the fifth larval instar. The polysomes, when supplemented with a soluble fraction from the posterior silk gland, exhibited the elongation reaction of the growing polypeptide-chains, but the initiation reaction of polypeptide synthesis was not demonstrated in this system. The predominant products synthesized on the membrane-bound polysomes were fibroin heavy chain (H-chain) and light chain (L-chain), while polypeptides of heterogeneous size classes were synthesized on the 105,000 X g-sedimentable polysomes. A substantial fraction of the fibroin L-chain synthesized was bound to the H-chain by disulfide bond. Most of the newly synthesized fibroin H- and L-chains on the membrane-bound polysomes were proved to be present within microsomal membrane vesicles because of their insensitivity to digestion with proteases in the absence of Triton X-100."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":134,"end":142},"obj":"Species:7091"},{"id":"T2","span":{"begin":144,"end":155},"obj":"Species:7091"},{"id":"T3","span":{"begin":233,"end":241},"obj":"Species:7091"},{"id":"T4","span":{"begin":243,"end":254},"obj":"Species:7091"},{"id":"T5","span":{"begin":632,"end":651},"obj":"Gene:693030"},{"id":"T6","span":{"begin":828,"end":843},"obj":"Gene:693047"},{"id":"T7","span":{"begin":884,"end":893},"obj":"Chemical:MESH:D004220"},{"id":"T8","span":{"begin":1125,"end":1137},"obj":"Chemical:MESH:D017830"}],"text":"Predominant synthesis of fibroin heavy and light chains on the membrane-bound polysomes prepared from the posterior silk gland of the silkworm, Bombyx mori.\nMembrane-bound polysomes were prepared from the posterior silk gland of the silkworm, Bombyx mori, on the fourth to fifth day in the fifth larval instar. The polysomes, when supplemented with a soluble fraction from the posterior silk gland, exhibited the elongation reaction of the growing polypeptide-chains, but the initiation reaction of polypeptide synthesis was not demonstrated in this system. The predominant products synthesized on the membrane-bound polysomes were fibroin heavy chain (H-chain) and light chain (L-chain), while polypeptides of heterogeneous size classes were synthesized on the 105,000 X g-sedimentable polysomes. A substantial fraction of the fibroin L-chain synthesized was bound to the H-chain by disulfide bond. Most of the newly synthesized fibroin H- and L-chains on the membrane-bound polysomes were proved to be present within microsomal membrane vesicles because of their insensitivity to digestion with proteases in the absence of Triton X-100."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":134,"end":142},"obj":"Species:7091"},{"id":"T2","span":{"begin":144,"end":155},"obj":"Species:7091"},{"id":"T3","span":{"begin":233,"end":241},"obj":"Species:7091"},{"id":"T4","span":{"begin":243,"end":254},"obj":"Species:7091"},{"id":"T5","span":{"begin":632,"end":651},"obj":"Gene:693030"},{"id":"T6","span":{"begin":828,"end":843},"obj":"Gene:693047"},{"id":"T7","span":{"begin":884,"end":893},"obj":"Chemical:MESH:D004220"},{"id":"T8","span":{"begin":1125,"end":1137},"obj":"Chemical:MESH:D017830"},{"id":"T11","span":{"begin":828,"end":843},"obj":"BMO_00548"},{"id":"T12","span":{"begin":632,"end":651},"obj":"BMO_00547"},{"id":"T14","span":{"begin":653,"end":660},"obj":"BMO_00547"},{"id":"T15","span":{"begin":873,"end":880},"obj":"BMO_00547"}],"text":"Predominant synthesis of fibroin heavy and light chains on the membrane-bound polysomes prepared from the posterior silk gland of the silkworm, Bombyx mori.\nMembrane-bound polysomes were prepared from the posterior silk gland of the silkworm, Bombyx mori, on the fourth to fifth day in the fifth larval instar. The polysomes, when supplemented with a soluble fraction from the posterior silk gland, exhibited the elongation reaction of the growing polypeptide-chains, but the initiation reaction of polypeptide synthesis was not demonstrated in this system. The predominant products synthesized on the membrane-bound polysomes were fibroin heavy chain (H-chain) and light chain (L-chain), while polypeptides of heterogeneous size classes were synthesized on the 105,000 X g-sedimentable polysomes. A substantial fraction of the fibroin L-chain synthesized was bound to the H-chain by disulfide bond. Most of the newly synthesized fibroin H- and L-chains on the membrane-bound polysomes were proved to be present within microsomal membrane vesicles because of their insensitivity to digestion with proteases in the absence of Triton X-100."}