PubMed:6488211 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":104},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":105,"end":249},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":250,"end":315},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":316,"end":496},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":497,"end":643},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":644,"end":770},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":771,"end":968},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":104},"obj":"Sentence"},{"id":"T2","span":{"begin":105,"end":249},"obj":"Sentence"},{"id":"T3","span":{"begin":250,"end":315},"obj":"Sentence"},{"id":"T4","span":{"begin":316,"end":496},"obj":"Sentence"},{"id":"T5","span":{"begin":497,"end":643},"obj":"Sentence"},{"id":"T6","span":{"begin":644,"end":770},"obj":"Sentence"},{"id":"T7","span":{"begin":771,"end":968},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Synthesis and fast-atom-bombardment-mass spectrometry of N-acetylmuramoyl-L-alanyl-D-isoglutamine (MDP).\nN-Acetylmuramoyl-L-alanyl-D-isoglutamine (MDP) was synthesized by a series of condensations of appropriate reagents, followed by hydrogenolysis. Each intermediate step resulted in a stable, crystalline product. D-Isoglutamine 4-benzyl ester was condensed with N-(tert-butoxycarbonyl)-L-alanine N-hydroxysuccinimide ester, to give N-(tert-butoxycarbonyl)-L-alanyl-D-isoglutamine benzyl ester. Condensation of L-alanyl-D-isoglutamine benzyl ester with N-acetyl-1-O-benzyl-4,6-O-benzylidenemuramic acid, followed by hydrogenolysis, gave MDP. The synthetic scheme was shown to be capable of producing gram quantities of highly pure MDP, as well as a few of its analogs. The synthetic MDP was characterized by analytical and biological methods, and it was found that the use of fast-atom-bombardment-mass spectrometry may greatly simplify the characterization process."}