| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-66 |
Sentence |
denotes |
Circular dichroism of intermediate subviral particles of reovirus. |
| T2 |
67-159 |
Sentence |
denotes |
Elucidation of the mechanism underlying the specific monovalent cation effects on uncoating. |
| T3 |
160-162 |
Sentence |
denotes |
1. |
| T4 |
163-314 |
Sentence |
denotes |
Circular dichroic (CD) spectra of purified intermediate subviral particles of reovirus were determined in the presence of different monovalent cations. |
| T5 |
315-317 |
Sentence |
denotes |
2. |
| T6 |
318-431 |
Sentence |
denotes |
The CD spectra reveal that reo intermediate subviral particles can exist in two conformationally different forms. |
| T7 |
432-651 |
Sentence |
denotes |
The two forms are readily distinguished by comparison of their ellipticities in the wavelength regions 210 nm and 220 nm, with a Na+-induced form exhibiting a reduced negative ellipticity relative to a Cs+-induced form. |
| T8 |
652-654 |
Sentence |
denotes |
3. |
| T9 |
655-825 |
Sentence |
denotes |
The transition between the Na+- and Cs+-induced forms is reversible by manipulation of the species of monovalent cation present and appears to be temperature independent. |
| T10 |
826-828 |
Sentence |
denotes |
4. |
| T11 |
829-1063 |
Sentence |
denotes |
Temperature variation studies on dilute suspensions of particles indicate that the Na+-induced form is stable, whereas the Cs+-induced from undergoes a second transition, temperature dependent and irreversible, to become a viral core. |
| T12 |
1064-1066 |
Sentence |
denotes |
5. |
| T13 |
1067-1191 |
Sentence |
denotes |
A model is presented relating these observations to the known properties of reovirus uncoating and transcriptase activation. |