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sentences

Id Subject Object Predicate Lexical cue
T1 0-97 Sentence denotes Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii.
T2 98-461 Sentence denotes In previous work from this laboratory, a partially purified protein kinase from the soil amoeba Acanthamoeba castellanii was shown to phosphorylate the heavy chain of the two single-headed Acanthamoeba myosin isoenzymes, myosin IA and IB, resulting in a 10- to 20-fold increase in their actin-activated Mg2+-ATPase activities (Maruta, H., and Korn, E.D. (1977) J.
T3 462-467 Sentence denotes Biol.
T4 468-473 Sentence denotes Chem.
T5 474-490 Sentence denotes 252, 8329-8332).
T6 491-706 Sentence denotes A myosin I heavy chain kinase has now been purified to near homogeneity from Acanthamoeba by chromatography on DE-52 cellulose, phosphocellulose, and Procion red dye, followed by chromatography on histone-Sepharose.
T7 707-807 Sentence denotes Myosin I heavy chain kinase contains a single polypeptide of 107,000 Da by electrophoretic analysis.
T8 808-987 Sentence denotes Molecular sieve chromatography yields a Stokes radius of 4.1 nm, consistent with a molecular weight of 107,000 for a native protein with a frictional ratio of approximately 1.3:1.
T9 988-1105 Sentence denotes The kinase catalyzes the incorporation of 0.9 to 1.0 mol of phosphate into the heavy chain of both myosins IA and IB.
T10 1106-1184 Sentence denotes Phosphoserine has been shown to be the phosphorylated amino acid in myosin IB.
T11 1185-1383 Sentence denotes The kinase has highest specific activity toward myosin IA and IB, about 3-4 mumol of phosphate incorporated/min/mg (30 degrees C) at concentrations of myosin I that are well below saturating levels.
T12 1384-1644 Sentence denotes The kinase also phosphorylates histone 2A, isolated smooth muscle light chains, and, to a very small extent, casein, but has no activity toward phosvitin or myosin II, a third Acanthamoeba myosin isoenzyme with a very different structure from myosin IA and IB.
T13 1645-1755 Sentence denotes Myosin I heavy chain kinase requires Mg2+ but is not dependent on Ca2+, Ca2+/calmodulin, or cAMP for activity.
T14 1756-1809 Sentence denotes The kinase undergoes an apparent autophosphorylation.
T1 0-97 Sentence denotes Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii.
T2 98-461 Sentence denotes In previous work from this laboratory, a partially purified protein kinase from the soil amoeba Acanthamoeba castellanii was shown to phosphorylate the heavy chain of the two single-headed Acanthamoeba myosin isoenzymes, myosin IA and IB, resulting in a 10- to 20-fold increase in their actin-activated Mg2+-ATPase activities (Maruta, H., and Korn, E.D. (1977) J.
T3 462-467 Sentence denotes Biol.
T4 468-473 Sentence denotes Chem.
T5 474-490 Sentence denotes 252, 8329-8332).
T6 491-706 Sentence denotes A myosin I heavy chain kinase has now been purified to near homogeneity from Acanthamoeba by chromatography on DE-52 cellulose, phosphocellulose, and Procion red dye, followed by chromatography on histone-Sepharose.
T7 707-807 Sentence denotes Myosin I heavy chain kinase contains a single polypeptide of 107,000 Da by electrophoretic analysis.
T8 808-987 Sentence denotes Molecular sieve chromatography yields a Stokes radius of 4.1 nm, consistent with a molecular weight of 107,000 for a native protein with a frictional ratio of approximately 1.3:1.
T9 988-1105 Sentence denotes The kinase catalyzes the incorporation of 0.9 to 1.0 mol of phosphate into the heavy chain of both myosins IA and IB.
T10 1106-1184 Sentence denotes Phosphoserine has been shown to be the phosphorylated amino acid in myosin IB.
T11 1185-1383 Sentence denotes The kinase has highest specific activity toward myosin IA and IB, about 3-4 mumol of phosphate incorporated/min/mg (30 degrees C) at concentrations of myosin I that are well below saturating levels.
T12 1384-1644 Sentence denotes The kinase also phosphorylates histone 2A, isolated smooth muscle light chains, and, to a very small extent, casein, but has no activity toward phosvitin or myosin II, a third Acanthamoeba myosin isoenzyme with a very different structure from myosin IA and IB.
T13 1645-1755 Sentence denotes Myosin I heavy chain kinase requires Mg2+ but is not dependent on Ca2+, Ca2+/calmodulin, or cAMP for activity.
T14 1756-1809 Sentence denotes The kinase undergoes an apparent autophosphorylation.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 280-286 http://purl.obolibrary.org/obo/MAT_0000294 denotes headed
T2 1436-1449 http://purl.obolibrary.org/obo/MAT_0000303 denotes smooth muscle
T3 1443-1449 http://purl.obolibrary.org/obo/MAT_0000025 denotes muscle

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 280-286 Body_part denotes headed http://purl.obolibrary.org/obo/MAT_0000294
T2 1436-1449 Body_part denotes smooth muscle http://purl.obolibrary.org/obo/MAT_0000303

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T5 568-580 OrganismTaxon denotes Acanthamoeba 5754
T6 1560-1572 OrganismTaxon denotes Acanthamoeba 5754
T1 72-96 OrganismTaxon denotes Acanthamoeba castellanii 5755
T2 187-193 OrganismTaxon denotes amoeba 5774
T3 194-218 OrganismTaxon denotes Acanthamoeba castellanii 5755
T4 287-299 OrganismTaxon denotes Acanthamoeba 5754

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 855-861 Body_part denotes radius http://purl.obolibrary.org/obo/UBERON_0001423
T2 1436-1449 Body_part denotes smooth muscle http://purl.obolibrary.org/obo/UBERON_0001135