PubMed:6288256 JSON TXT

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Test-Species-PubTator

{"project":"Test-Species-PubTator","denotations":[{"id":"3","span":{"begin":696,"end":698},"obj":"CellLine"},{"id":"4","span":{"begin":902,"end":904},"obj":"CellLine"},{"id":"5","span":{"begin":1347,"end":1349},"obj":"CellLine"}],"attributes":[{"id":"A3","pred":"resolved_to","subj":"3","obj":"CVCL:0194"},{"id":"A4","pred":"resolved_to","subj":"4","obj":"CVCL:0194"},{"id":"A5","pred":"resolved_to","subj":"5","obj":"CVCL:0194"}],"text":"A small subclass of SV40 T antigen binds to the viral origin of replication.\nWe examined the affinities of SV40 large T antigen for unique viral DNA sequences by binding SV40 Bst NI DNA fragments in extracts of infected or transformed cells, and then immunoprecipitating the T antigen-DNA complex. The G fragment, which spans the viral origin of replication (ori) was quantitatively bound to T antigen. A T-antigen-specific monoclonal antibody (McI 7), which recognized only 5%-10% of the T antigen from infected or transformed cells, immunoprecipitated the majority of the ori-binding activity. This suggests that only a minor subclass of wild-type T antigen is active in binding to the origin. C6 cells contain a replication-defective mutant T antigen that when tested in the DNA-binding immunoassay, showed no affinity for the ori fragment. McI 7 not only failed to immunoprecipitate ori binding in C6 cells, but also did not detect any labeled C6 T antigen whatever. Thus McI 7 recognizes an immunologically distinct subset of wild-type 7 antigen that comprises the origin-binding form of the viral protein, which is absent in the C6 T antigen population. McI 122, which recognizes a 53 kilodalton host protein that complexes with T antigen, immunoprecipitated ori-binding activity from extracts of infected or transformed cells, but not from C6 cells. Thus wild-type T antigen can bind ori sequences even when complexed to the host protein. These data suggest that T antigen consists of different subpopulations with different functions."}

Test-Species-PubDictionaries-PubMedBERT

{"project":"Test-Species-PubDictionaries-PubMedBERT","denotations":[{"id":"T1","span":{"begin":359,"end":362},"obj":"Species"},{"id":"T2","span":{"begin":574,"end":577},"obj":"Species"},{"id":"T3","span":{"begin":622,"end":627},"obj":"Species"},{"id":"T4","span":{"begin":830,"end":833},"obj":"Species"},{"id":"T5","span":{"begin":887,"end":890},"obj":"Species"},{"id":"T6","span":{"begin":916,"end":920},"obj":"Species"},{"id":"T7","span":{"begin":1265,"end":1268},"obj":"Species"},{"id":"T8","span":{"begin":1391,"end":1394},"obj":"Species"},{"id":"T9","span":{"begin":1452,"end":1456},"obj":"Species"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"2492376"},{"id":"A2","pred":"db_id","subj":"T2","obj":"2492376"},{"id":"A3","pred":"db_id","subj":"T3","obj":"1925466"},{"id":"A4","pred":"db_id","subj":"T4","obj":"2492376"},{"id":"A5","pred":"db_id","subj":"T5","obj":"2492376"},{"id":"A6","pred":"db_id","subj":"T6","obj":"209674"},{"id":"A7","pred":"db_id","subj":"T7","obj":"2492376"},{"id":"A8","pred":"db_id","subj":"T8","obj":"2492376"},{"id":"A9","pred":"db_id","subj":"T9","obj":"1369087"}],"text":"A small subclass of SV40 T antigen binds to the viral origin of replication.\nWe examined the affinities of SV40 large T antigen for unique viral DNA sequences by binding SV40 Bst NI DNA fragments in extracts of infected or transformed cells, and then immunoprecipitating the T antigen-DNA complex. The G fragment, which spans the viral origin of replication (ori) was quantitatively bound to T antigen. A T-antigen-specific monoclonal antibody (McI 7), which recognized only 5%-10% of the T antigen from infected or transformed cells, immunoprecipitated the majority of the ori-binding activity. This suggests that only a minor subclass of wild-type T antigen is active in binding to the origin. C6 cells contain a replication-defective mutant T antigen that when tested in the DNA-binding immunoassay, showed no affinity for the ori fragment. McI 7 not only failed to immunoprecipitate ori binding in C6 cells, but also did not detect any labeled C6 T antigen whatever. Thus McI 7 recognizes an immunologically distinct subset of wild-type 7 antigen that comprises the origin-binding form of the viral protein, which is absent in the C6 T antigen population. McI 122, which recognizes a 53 kilodalton host protein that complexes with T antigen, immunoprecipitated ori-binding activity from extracts of infected or transformed cells, but not from C6 cells. Thus wild-type T antigen can bind ori sequences even when complexed to the host protein. These data suggest that T antigen consists of different subpopulations with different functions."}

GlyCosmos15-Glycan

Glycan-GlyCosmos

GlyCosmos-GlycoEpitope

GlyCosmos15-GlycoEpitope

GlyCosmos15-Sentences

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":76},"obj":"Sentence"},{"id":"T2","span":{"begin":77,"end":297},"obj":"Sentence"},{"id":"T3","span":{"begin":298,"end":402},"obj":"Sentence"},{"id":"T4","span":{"begin":403,"end":595},"obj":"Sentence"},{"id":"T5","span":{"begin":596,"end":695},"obj":"Sentence"},{"id":"T6","span":{"begin":696,"end":843},"obj":"Sentence"},{"id":"T7","span":{"begin":844,"end":970},"obj":"Sentence"},{"id":"T8","span":{"begin":971,"end":1159},"obj":"Sentence"},{"id":"T9","span":{"begin":1160,"end":1356},"obj":"Sentence"},{"id":"T10","span":{"begin":1357,"end":1445},"obj":"Sentence"},{"id":"T11","span":{"begin":1446,"end":1542},"obj":"Sentence"}],"text":"A small subclass of SV40 T antigen binds to the viral origin of replication.\nWe examined the affinities of SV40 large T antigen for unique viral DNA sequences by binding SV40 Bst NI DNA fragments in extracts of infected or transformed cells, and then immunoprecipitating the T antigen-DNA complex. The G fragment, which spans the viral origin of replication (ori) was quantitatively bound to T antigen. A T-antigen-specific monoclonal antibody (McI 7), which recognized only 5%-10% of the T antigen from infected or transformed cells, immunoprecipitated the majority of the ori-binding activity. This suggests that only a minor subclass of wild-type T antigen is active in binding to the origin. C6 cells contain a replication-defective mutant T antigen that when tested in the DNA-binding immunoassay, showed no affinity for the ori fragment. McI 7 not only failed to immunoprecipitate ori binding in C6 cells, but also did not detect any labeled C6 T antigen whatever. Thus McI 7 recognizes an immunologically distinct subset of wild-type 7 antigen that comprises the origin-binding form of the viral protein, which is absent in the C6 T antigen population. McI 122, which recognizes a 53 kilodalton host protein that complexes with T antigen, immunoprecipitated ori-binding activity from extracts of infected or transformed cells, but not from C6 cells. Thus wild-type T antigen can bind ori sequences even when complexed to the host protein. These data suggest that T antigen consists of different subpopulations with different functions."}