Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-34 |
Sentence |
denotes |
Control of glycoprotein synthesis. |
T2 |
35-248 |
Sentence |
denotes |
UDP-GlcNAc:glycopeptide beta 4-N-acetylglucosaminyltransferase III, an enzyme in hen oviduct which adds GlcNAc in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-glycosyl oligosaccharides. |
T3 |
249-323 |
Sentence |
denotes |
Hen oviduct membranes are shown to catalyze the following enzyme reaction: |
T4 |
324-590 |
Sentence |
denotes |
GlcNAc beta 1-2Man alpha 1-6(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-Asn + UDP-GlcNAc leads to GlcNAc beta 1-2Man alpha 1-6(GlcNAc beta 1-2Man alpha 1-3)GlcNAc beta 1-4)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-Asn + UDP. |
T5 |
591-875 |
Sentence |
denotes |
The enzyme catalyzing this reaction has been named UDP-GlcNAc:glycopeptide beta 4-N-acetylglucosaminyltransferase III (GlcNAc-transferase III) to distinguish it from two other GlcNAc-transferases (I and II) present in hen oviduct and previously described in several mammalian tissues. |
T6 |
876-1034 |
Sentence |
denotes |
GlcNAc-transferases I and II, respectively, attach GlcNAc in beta 1-2 linkage to the Man alpha 1-3 and Man alpha 1-6 arms of Asn-linked oligosaccharide cores. |
T7 |
1035-1248 |
Sentence |
denotes |
A specific assay for GlcNAc-transferase III was devised by using concanavalin A/Sepharose columns to separate the product of transferase III from other interfering radioactive glycopeptides formed in the reaction. |
T8 |
1249-1354 |
Sentence |
denotes |
The specific activity of GlcNAc-transferase III in hen oviduct membranes is about 5 nmol/mg of protein/h. |
T9 |
1355-1517 |
Sentence |
denotes |
Substrate specificity studies have shown that GlcNAc-transferase III requires both terminal beta 1-2-linked GlcNAc residues in its substrate for maximal activity. |
T10 |
1518-1675 |
Sentence |
denotes |
Removal of the GlcNAc residue on the Man alpha 1-6 arm reduces activity by at least 85% and removal of both GlcNAc residues reduces activity by at least 93%. |
T11 |
1676-1874 |
Sentence |
denotes |
Two large scale preparations of product were subjected to high resolution proton NMR spectroscopy to establish the incorporation by the enzyme of a GlcNAc in beta 1-4 linkage to the beta-linked Man. |
T12 |
1875-2030 |
Sentence |
denotes |
This GlcNAc residue is called a "bisecting" GlcNAc and appears to play important control functions in the synthesis of complex N-glycosyl oligosaccharides. |
T13 |
2031-2157 |
Sentence |
denotes |
Several enzymes in the biosynthetic scheme are unable to act on glycopeptide substrates containing a bisecting GlcNAc residue. |
T1 |
0-34 |
Sentence |
denotes |
Control of glycoprotein synthesis. |
T2 |
35-248 |
Sentence |
denotes |
UDP-GlcNAc:glycopeptide beta 4-N-acetylglucosaminyltransferase III, an enzyme in hen oviduct which adds GlcNAc in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-glycosyl oligosaccharides. |
T3 |
249-323 |
Sentence |
denotes |
Hen oviduct membranes are shown to catalyze the following enzyme reaction: |
T4 |
324-590 |
Sentence |
denotes |
GlcNAc beta 1-2Man alpha 1-6(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-Asn + UDP-GlcNAc leads to GlcNAc beta 1-2Man alpha 1-6(GlcNAc beta 1-2Man alpha 1-3)GlcNAc beta 1-4)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-Asn + UDP. |
T5 |
591-875 |
Sentence |
denotes |
The enzyme catalyzing this reaction has been named UDP-GlcNAc:glycopeptide beta 4-N-acetylglucosaminyltransferase III (GlcNAc-transferase III) to distinguish it from two other GlcNAc-transferases (I and II) present in hen oviduct and previously described in several mammalian tissues. |
T6 |
876-1034 |
Sentence |
denotes |
GlcNAc-transferases I and II, respectively, attach GlcNAc in beta 1-2 linkage to the Man alpha 1-3 and Man alpha 1-6 arms of Asn-linked oligosaccharide cores. |
T7 |
1035-1248 |
Sentence |
denotes |
A specific assay for GlcNAc-transferase III was devised by using concanavalin A/Sepharose columns to separate the product of transferase III from other interfering radioactive glycopeptides formed in the reaction. |
T8 |
1249-1354 |
Sentence |
denotes |
The specific activity of GlcNAc-transferase III in hen oviduct membranes is about 5 nmol/mg of protein/h. |
T9 |
1355-1517 |
Sentence |
denotes |
Substrate specificity studies have shown that GlcNAc-transferase III requires both terminal beta 1-2-linked GlcNAc residues in its substrate for maximal activity. |
T10 |
1518-1675 |
Sentence |
denotes |
Removal of the GlcNAc residue on the Man alpha 1-6 arm reduces activity by at least 85% and removal of both GlcNAc residues reduces activity by at least 93%. |
T11 |
1676-1874 |
Sentence |
denotes |
Two large scale preparations of product were subjected to high resolution proton NMR spectroscopy to establish the incorporation by the enzyme of a GlcNAc in beta 1-4 linkage to the beta-linked Man. |
T12 |
1875-2030 |
Sentence |
denotes |
This GlcNAc residue is called a "bisecting" GlcNAc and appears to play important control functions in the synthesis of complex N-glycosyl oligosaccharides. |
T13 |
2031-2157 |
Sentence |
denotes |
Several enzymes in the biosynthetic scheme are unable to act on glycopeptide substrates containing a bisecting GlcNAc residue. |