PubMed:6198387 JSONTXT

{"project":"AnEM_abstracts","denotations":[{"id":"T1","span":{"begin":42,"end":48},"obj":"Cell"},{"id":"T2","span":{"begin":123,"end":129},"obj":"Cell"},{"id":"T3","span":{"begin":178,"end":185},"obj":"Cell"},{"id":"T4","span":{"begin":218,"end":238},"obj":"Cell"},{"id":"T5","span":{"begin":240,"end":242},"obj":"Cell"},{"id":"T6","span":{"begin":318,"end":320},"obj":"Cell"},{"id":"T7","span":{"begin":398,"end":404},"obj":"Cell"},{"id":"T8","span":{"begin":583,"end":585},"obj":"Cell"},{"id":"T9","span":{"begin":979,"end":1013},"obj":"Cell"},{"id":"T10","span":{"begin":1072,"end":1081},"obj":"Cell"},{"id":"T11","span":{"begin":1192,"end":1202},"obj":"Cell"},{"id":"T12","span":{"begin":1287,"end":1294},"obj":"Cell"},{"id":"T13","span":{"begin":1325,"end":1327},"obj":"Cell"},{"id":"T14","span":{"begin":1361,"end":1363},"obj":"Cell"},{"id":"T15","span":{"begin":1395,"end":1401},"obj":"Cell"},{"id":"T16","span":{"begin":1428,"end":1430},"obj":"Cell"},{"id":"T17","span":{"begin":1465,"end":1472},"obj":"Cell"},{"id":"T18","span":{"begin":1522,"end":1524},"obj":"Cell"},{"id":"T19","span":{"begin":1563,"end":1565},"obj":"Cell"},{"id":"T20","span":{"begin":1615,"end":1617},"obj":"Cell"},{"id":"T21","span":{"begin":1661,"end":1668},"obj":"Cell"},{"id":"T22","span":{"begin":1792,"end":1794},"obj":"Cell"},{"id":"T23","span":{"begin":1820,"end":1827},"obj":"Cell"},{"id":"T24","span":{"begin":1944,"end":1957},"obj":"Cell"},{"id":"T25","span":{"begin":295,"end":297},"obj":"Cell"},{"id":"T26","span":{"begin":870,"end":872},"obj":"Cell"},{"id":"T27","span":{"begin":765,"end":767},"obj":"Cell"}],"text":"Role of interleukin 1 in antigen-specific T cell proliferation.\nThe role of interleukin 1 (IL 1) in human antigen-specific T cell proliferation was examined. Nylon wool-purified T cells proliferated in the presence of autologous monocytes (Mo.) pulsed for 18 h with tetanus toxoid (TT) antigen (Mo.TT). Irradiation of Mo.TT with ultraviolet (UV) light (72 J/m2) abolished their capacity to support T cell proliferation and drastically reduced their capacity to secrete IL 1 after stimulation with Staphylococcus albus. The defect in antigen presentation induced by UV irradiation of Mo.TT was reversed in a dose-dependent manner by the addition of two different preparations containing human interleukin 1 (IL 1). The first preparation consisted of supernatants of Mo. stimulated with Con A for 18 hr and in which Con A activity was blocked by alpha-D-methyl-mannoside (Mo.-Con A-Sup). The second preparation consisted of human IL 1 partially purified from supernatants of human peripheral blood mononuclear cells stimulated with S. albus. This IL 1 copurified with human leukocyte pyrogen (LP) and was termed IL 1/LP. Both IL 1-containing preparations enhanced the response of C57BL/6 mouse thymocytes to phytohemagglutinin. A rabbit antibody to human IL 1/LP inhibited the capacity of T cells to proliferate in response to Mo.TT and inhibited the capacity of Mo.-Con A-Sup to reconstitute the T cell response to UV-irradiated Mo.TT. IL 1/LP was not necessary for T cells to recognize the immunogenic moiety presented by Mo., because monolayers of UV-irradiated Mo.TT were equivalent to monolayers of unirradiated MO.TT in their capacity to adsorb TT-reactive T cells specifically. Furthermore, the addition of rabbit antibody to IL 1/LP did not interfere with the capacity of UV-irradiated Mo.TT to adsorb TT-reactive T cells. The results obtained in this study indicate that IL 1 is involved in optimal antigen-driven proliferation of human T lymphocytes."}