| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-85 |
Sentence |
denotes |
Inhibition of smooth muscle actin-activated myosin Mg2+-ATPase activity by caldesmon. |
| T2 |
86-227 |
Sentence |
denotes |
Caldesmon, a major calmodulin- and actin-binding protein of smooth muscle (Sobue, K., Muramoto, Y., Fujita, M., and Kakiuchi, S. (1981) Proc. |
| T3 |
228-233 |
Sentence |
denotes |
Natl. |
| T4 |
234-239 |
Sentence |
denotes |
Acad. |
| T5 |
240-244 |
Sentence |
denotes |
Sci. |
| T6 |
245-247 |
Sentence |
denotes |
U. |
| T7 |
248-250 |
Sentence |
denotes |
S. |
| T8 |
251-253 |
Sentence |
denotes |
A. |
| T9 |
254-396 |
Sentence |
denotes |
78, 5652-5655), has been obtained in highly purified form from chicken gizzard by a modification of a previously published procedure (Ngai, P. |
| T10 |
397-415 |
Sentence |
denotes |
K., Carruthers, C. |
| T11 |
416-433 |
Sentence |
denotes |
A., and Walsh, M. |
| T12 |
434-452 |
Sentence |
denotes |
P. (1984) Biochem. |
| T13 |
453-455 |
Sentence |
denotes |
J. |
| T14 |
456-729 |
Sentence |
denotes |
218, 863-870) and was found to cause a significant inhibition of both superprecipitation and actin-activated myosin Mg2+-ATPase activity in a system reconstituted from the purified contractile and regulatory proteins without influencing the phosphorylation state of myosin. |
| T15 |
730-810 |
Sentence |
denotes |
This inhibitory effect was seen both in the presence and absence of tropomyosin. |
| T16 |
811-985 |
Sentence |
denotes |
A Ca2+-and calmodulin-dependent kinase which catalyzed phosphorylation of caldesmon was identified in chicken gizzard; this kinase is distinct from myosin light-chain kinase. |
| T17 |
986-1176 |
Sentence |
denotes |
Caldesmon prepared by calmodulin-Sepharose affinity chromatography was contaminated with caldesmon kinase activity and was unable to inhibit actomyosin ATPase activity or superprecipitation. |
| T18 |
1177-1274 |
Sentence |
denotes |
Phosphatase activity capable of dephosphorylating caldesmon was also identified in smooth muscle. |
| T19 |
1275-1473 |
Sentence |
denotes |
These results indicate that caldesmon can inhibit smooth muscle actomyosin ATPase activity in vitro, and this function may itself be subject to regulation by reversible phosphorylation of caldesmon. |
| T1 |
0-85 |
Sentence |
denotes |
Inhibition of smooth muscle actin-activated myosin Mg2+-ATPase activity by caldesmon. |
| T2 |
86-227 |
Sentence |
denotes |
Caldesmon, a major calmodulin- and actin-binding protein of smooth muscle (Sobue, K., Muramoto, Y., Fujita, M., and Kakiuchi, S. (1981) Proc. |
| T3 |
228-233 |
Sentence |
denotes |
Natl. |
| T4 |
234-239 |
Sentence |
denotes |
Acad. |
| T5 |
240-244 |
Sentence |
denotes |
Sci. |
| T6 |
245-247 |
Sentence |
denotes |
U. |
| T7 |
248-250 |
Sentence |
denotes |
S. |
| T8 |
251-253 |
Sentence |
denotes |
A. |
| T9 |
254-396 |
Sentence |
denotes |
78, 5652-5655), has been obtained in highly purified form from chicken gizzard by a modification of a previously published procedure (Ngai, P. |
| T10 |
397-415 |
Sentence |
denotes |
K., Carruthers, C. |
| T11 |
416-433 |
Sentence |
denotes |
A., and Walsh, M. |
| T12 |
434-452 |
Sentence |
denotes |
P. (1984) Biochem. |
| T13 |
453-455 |
Sentence |
denotes |
J. |
| T14 |
456-729 |
Sentence |
denotes |
218, 863-870) and was found to cause a significant inhibition of both superprecipitation and actin-activated myosin Mg2+-ATPase activity in a system reconstituted from the purified contractile and regulatory proteins without influencing the phosphorylation state of myosin. |
| T15 |
730-810 |
Sentence |
denotes |
This inhibitory effect was seen both in the presence and absence of tropomyosin. |
| T16 |
811-985 |
Sentence |
denotes |
A Ca2+-and calmodulin-dependent kinase which catalyzed phosphorylation of caldesmon was identified in chicken gizzard; this kinase is distinct from myosin light-chain kinase. |
| T17 |
986-1176 |
Sentence |
denotes |
Caldesmon prepared by calmodulin-Sepharose affinity chromatography was contaminated with caldesmon kinase activity and was unable to inhibit actomyosin ATPase activity or superprecipitation. |
| T18 |
1177-1274 |
Sentence |
denotes |
Phosphatase activity capable of dephosphorylating caldesmon was also identified in smooth muscle. |
| T19 |
1275-1473 |
Sentence |
denotes |
These results indicate that caldesmon can inhibit smooth muscle actomyosin ATPase activity in vitro, and this function may itself be subject to regulation by reversible phosphorylation of caldesmon. |