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PubMed:6085029 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-115 Sentence denotes Specific and non-specific affinities of the extracellular glucosyltransferase complex of Streptococcus mutans 6715.
TextSentencer_T2 116-275 Sentence denotes Glucosyltransferases (GTF) from different strains of streptococci exhibited different elution profiles when fractionated on insoluble-dextran affinity columns.
TextSentencer_T3 276-532 Sentence denotes The proportions of unadsorbed and adsorbed GTF were not related to their extent of stimulation by exogenous dextran, and GTF preparations exposed to, and freed from, clinical dextran prior to fractionation lost their ability to bind to the dextran columns.
TextSentencer_T4 533-866 Sentence denotes Different proportions of bound GTF were released by irrigation of columns with different concentrations of salt and clinical dextran, and the "specific" binding and release of GTF exhibited by a column possessing covalently linked, clinical dextran ligands was duplicated on a control column that did not possess the dextran ligands.
TextSentencer_T5 867-1117 Sentence denotes These results, and the high affinity of GTF for hydrophobic alkyl (Shaltiel) ligands, demonstrate that ionic and hydrophobic properties of impure GTF aggregates may lead to erroneous characterization of the dextran affinity of some protein fractions.
TextSentencer_T6 1118-1364 Sentence denotes Fractionations on DEAE-Sepharose and on hydroxylapatite showed that the two dextran-dependent GTF activities (GTF-S and GTF-I) were present in the major enzyme fraction (Streptococcus mutans 6715) recovered from a Sephacryl S-200 affinity column.
TextSentencer_T7 1365-1441 Sentence denotes A minor, dextran-independent GTF was not adsorbed onto the Sephacryl column.
TextSentencer_T8 1442-1653 Sentence denotes The presence of SDS (0.005%) and Triton X100 (0.01%) stabilized GTF activity during gel filtration and improved the separation of GTF-S and GTF-I in hydroxylapatite fractionation of the highly aggregated enzyme.
TextSentencer_T9 1654-1827 Sentence denotes A comparable separation of the two enzyme forms on DEAE-Sepharose was achieved only if T10 dextran (10 mg/mL) was included with the detergent mixture in the column irrigant.
T1 0-115 Sentence denotes Specific and non-specific affinities of the extracellular glucosyltransferase complex of Streptococcus mutans 6715.
T2 116-275 Sentence denotes Glucosyltransferases (GTF) from different strains of streptococci exhibited different elution profiles when fractionated on insoluble-dextran affinity columns.
T3 276-532 Sentence denotes The proportions of unadsorbed and adsorbed GTF were not related to their extent of stimulation by exogenous dextran, and GTF preparations exposed to, and freed from, clinical dextran prior to fractionation lost their ability to bind to the dextran columns.
T4 533-866 Sentence denotes Different proportions of bound GTF were released by irrigation of columns with different concentrations of salt and clinical dextran, and the "specific" binding and release of GTF exhibited by a column possessing covalently linked, clinical dextran ligands was duplicated on a control column that did not possess the dextran ligands.
T5 867-1117 Sentence denotes These results, and the high affinity of GTF for hydrophobic alkyl (Shaltiel) ligands, demonstrate that ionic and hydrophobic properties of impure GTF aggregates may lead to erroneous characterization of the dextran affinity of some protein fractions.
T6 1118-1364 Sentence denotes Fractionations on DEAE-Sepharose and on hydroxylapatite showed that the two dextran-dependent GTF activities (GTF-S and GTF-I) were present in the major enzyme fraction (Streptococcus mutans 6715) recovered from a Sephacryl S-200 affinity column.
T7 1365-1441 Sentence denotes A minor, dextran-independent GTF was not adsorbed onto the Sephacryl column.
T8 1442-1653 Sentence denotes The presence of SDS (0.005%) and Triton X100 (0.01%) stabilized GTF activity during gel filtration and improved the separation of GTF-S and GTF-I in hydroxylapatite fractionation of the highly aggregated enzyme.
T9 1654-1827 Sentence denotes A comparable separation of the two enzyme forms on DEAE-Sepharose was achieved only if T10 dextran (10 mg/mL) was included with the detergent mixture in the column irrigant.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 89-109 OrganismTaxon denotes Streptococcus mutans 1309
T2 1288-1308 OrganismTaxon denotes Streptococcus mutans 1309

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 44-57 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576