PubMed:5334765 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/5334765","sourcedb":"PubMed","sourceid":"5334765","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/5334765","text":"Differentiation of cariogenic streptococci by fluorescent antibody.\nJablon, J. M. (University of Miami, Miami, Fla.), and D. D. Zinner. Differentiation of cariogenic streptococci by fluorescent antibody. J. Bacteriol. 92:1590-1596. 1966.-Eight strains of streptococci were isolated from human carious lesions by the fluorescent-antibody (FA) technique. Seven of these strains produced experimental caries in hamsters or rats maintained on a high sucrose diet. The eighth strain was noncariogenic in animals but possessed some antigenic components in common with the cariogenic strains. On the basis of antigen-antibody reactions by microprecipitin and agar-gel diffusion patterns, the strains were divided into four groups; these groups differed with regard to their cariogenic activity in hamsters. Fluorescein-conjugated antisera, prepared against the human strains, showed some cross-reactions which interfered with the efficacy of the FA technique in differentiating between the related streptococcal groups. To eliminate these cross-reactions, a small amount of related-strain antisera was added to the fluorescein-conjugated antisera to the cariogenic strains. This technique is effective in blocking cross-reactions and should be tried wherever cross-reactions are encountered in the FA technique.","tracks":[{"project":"Ab3P-abbreviations","denotations":[{"id":"SF0","span":{"begin":338,"end":340},"obj":"ABBR"},{"id":"LF0","span":{"begin":316,"end":336},"obj":"ABBR"}],"relations":[{"id":"R0","pred":"ShortForm","subj":"LF0","obj":"SF0"}],"attributes":[{"subj":"SF0","pred":"source","obj":"Ab3P-abbreviations"},{"subj":"LF0","pred":"source","obj":"Ab3P-abbreviations"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Ab3P-abbreviations","color":"#ec9793","default":true}]}]}}