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PubMed:415761 JSONTXT

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Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 19-26 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T2 506-513 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T3 690-697 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 12-18 OrganismTaxon denotes bovine 9913
T2 92-98 OrganismTaxon denotes Bovine 9913
T3 453-459 OrganismTaxon denotes bovine 9913

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-36 Sentence denotes Immobilized bovine lactose synthase.
T2 37-91 Sentence denotes A method of topographical analysis of the active site.
T3 92-334 Sentence denotes Bovine galactosyltransferase (UDPgalactose: D-glucose 4beta-galactosyltransferase, EC 2.4.1.22) was covalently coupled to Sepharose 4B by reaction at pH 5.0 with the activated mixed disulfide Sepharose-glutathione-2(5-nitropyridyl)-disulfide.
T4 335-467 Sentence denotes The Sepharose-protein conjugate was presumably coupled via the unique highly reactive cysteine of those thiols on the bovine enzyme.
T5 468-676 Sentence denotes The gel-bound N-acetyllactosamine and lactose synthase activity of about 0.4% was consistent with the affects of diffusion and the 90% activity reduction noted upon thiol modification of the dissolved enzyme.
T6 677-929 Sentence denotes The residual lactose biosynthetic activity of the bound enzyme appeared possible only if the reactive thiol were physically distinct from the active site since the bulky Sepharose-glutathione group must not obscure the alpha-lactalbumin binding region.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 19-26 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T2 506-513 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T3 690-697 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 12-18 OrganismTaxon denotes bovine 9913
T2 92-98 OrganismTaxon denotes Bovine 9913
T3 453-459 OrganismTaxon denotes bovine 9913