PubMed:4019466
Annnotations
GlyCosmos6-Glycan-Motif-Image
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 165-172 | Glycan_Motif | denotes | heparin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO |
T3 | 367-374 | Glycan_Motif | denotes | heparin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO |
T5 | 394-415 | Glycan_Motif | denotes | chondroitin 6-sulfate | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G34992DF |
T6 | 394-405 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
T7 | 652-659 | Glycan_Motif | denotes | heparin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO |
T9 | 664-675 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
T10 | 843-850 | Glycan_Motif | denotes | heparin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO |
T12 | 889-900 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
Glycosmos6-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 394-415 | http://www.glycoepitope.jp/epitopes/EP0083 | denotes | chondroitin 6-sulfate |
T2 | 394-405 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
T3 | 664-675 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
T4 | 889-900 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-82 | Sentence | denotes | A sulfatase specific for glucuronic acid 2-sulfate residues in glycosaminoglycans. |
T2 | 83-269 | Sentence | denotes | Although 2-O-sulfated L-iduronic acid (IdoA) residues have been known to occur in heparin, 2-O-sulfated D-glucuronic acid (GlcA) residues have been reported only recently (Bienkowski, M. |
T3 | 270-288 | Sentence | denotes | J., and Conrad, H. |
T4 | 289-301 | Sentence | denotes | E. (1985) J. |
T5 | 302-307 | Sentence | denotes | Biol. |
T6 | 308-313 | Sentence | denotes | Chem. |
T7 | 314-328 | Sentence | denotes | 250, 356-365). |
T8 | 329-566 | Sentence | denotes | Disaccharides prepared by cleavage of heparin and N-deacetylated chondroitin 6-sulfate with nitrous acid were used to demonstrate a new sulfatase that catalyzed the removal of the 2-O-sulfate substituents from GlcA but not IdoA residues. |
T9 | 567-808 | Sentence | denotes | The deamination products were labeled by NaB3H4 reduction to give disaccharides from heparin and chondroitin sulfate which had reducing terminal 2,5-anhydro-D-mannitol ([3H]AManR) and 2,5-anhydro-D-talitol ([3H]ATalR) residues, respectively. |
T10 | 809-945 | Sentence | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) from heparin and GlcA(2-SO4)-[3H]ATalR(6-SO4) from chondroitin sulfate were purified for use as substrates. |
T11 | 946-1069 | Sentence | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) was prepared by epimerization of IdoA(2-SO4)-[3H]AManR(6-SO4) with hydrazine at 100 degrees C. |
T12 | 1070-1355 | Sentence | denotes | Lysosomal enzyme preparations from chick embryo chondrocytes and from two normal human fibroblast cell lines catalyzed the removal of the 2-O-SO4 substituent from the uronic acid residues of IdoA(2-SO4)-[3H]AManR(6-SO4), GlcA(2-SO4)-[3H] AManR(6-SO4), and GlcA(2-SO4)-[3H]ATalR(6-SO4). |
T13 | 1356-1646 | Sentence | denotes | In contrast, a lysosomal enzyme preparation from a human fibroblast cell line deficient in idurono-2-sulfatase (Hunter's-syndrome), which had no activity on the IdoA(2-SO4)-[3H]AManR(6-SO4), converted GlcA(2-SO4)-[3H]AManR(6-SO4) to a mixture of GlcA-[3H] AManR(6-SO4) and [3H]AManR(6-SO4). |
T14 | 1647-1762 | Sentence | denotes | This enzyme also converted GlcA(2-SO4)-[3H]ATalR(6-SO4) to a mixture of GlcA-[3H]ATalR(6-SO4) and [3H]ATalR(6-SO4). |
T15 | 1763-1947 | Sentence | denotes | Digestion of both GlcA(2-SO4)-[3H]AManR(6-SO4) and GlcA(2-SO4)-[3H]ATalR(6-SO4) was inhibited by 35SO2-4 and was arrested at the monosulfated disaccharide stage by 1,4-saccharolactone. |
T16 | 1948-2002 | Sentence | denotes | The glucurono-2-sulfatase exhibited a pH optimum of 4. |
T17 | 2003-2151 | Sentence | denotes | The results indicate that there exists a separate sulfatase for the removal of sulfate substituents from C-2 of GlcA residues in glycosaminoglycans. |
T1 | 0-82 | Sentence | denotes | A sulfatase specific for glucuronic acid 2-sulfate residues in glycosaminoglycans. |
T2 | 83-269 | Sentence | denotes | Although 2-O-sulfated L-iduronic acid (IdoA) residues have been known to occur in heparin, 2-O-sulfated D-glucuronic acid (GlcA) residues have been reported only recently (Bienkowski, M. |
T3 | 270-288 | Sentence | denotes | J., and Conrad, H. |
T4 | 289-301 | Sentence | denotes | E. (1985) J. |
T5 | 302-307 | Sentence | denotes | Biol. |
T6 | 308-313 | Sentence | denotes | Chem. |
T7 | 314-328 | Sentence | denotes | 250, 356-365). |
T8 | 329-566 | Sentence | denotes | Disaccharides prepared by cleavage of heparin and N-deacetylated chondroitin 6-sulfate with nitrous acid were used to demonstrate a new sulfatase that catalyzed the removal of the 2-O-sulfate substituents from GlcA but not IdoA residues. |
T9 | 567-808 | Sentence | denotes | The deamination products were labeled by NaB3H4 reduction to give disaccharides from heparin and chondroitin sulfate which had reducing terminal 2,5-anhydro-D-mannitol ([3H]AManR) and 2,5-anhydro-D-talitol ([3H]ATalR) residues, respectively. |
T10 | 809-945 | Sentence | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) from heparin and GlcA(2-SO4)-[3H]ATalR(6-SO4) from chondroitin sulfate were purified for use as substrates. |
T11 | 946-1069 | Sentence | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) was prepared by epimerization of IdoA(2-SO4)-[3H]AManR(6-SO4) with hydrazine at 100 degrees C. |
T12 | 1070-1355 | Sentence | denotes | Lysosomal enzyme preparations from chick embryo chondrocytes and from two normal human fibroblast cell lines catalyzed the removal of the 2-O-SO4 substituent from the uronic acid residues of IdoA(2-SO4)-[3H]AManR(6-SO4), GlcA(2-SO4)-[3H] AManR(6-SO4), and GlcA(2-SO4)-[3H]ATalR(6-SO4). |
T13 | 1356-1646 | Sentence | denotes | In contrast, a lysosomal enzyme preparation from a human fibroblast cell line deficient in idurono-2-sulfatase (Hunter's-syndrome), which had no activity on the IdoA(2-SO4)-[3H]AManR(6-SO4), converted GlcA(2-SO4)-[3H]AManR(6-SO4) to a mixture of GlcA-[3H] AManR(6-SO4) and [3H]AManR(6-SO4). |
T14 | 1647-1762 | Sentence | denotes | This enzyme also converted GlcA(2-SO4)-[3H]ATalR(6-SO4) to a mixture of GlcA-[3H]ATalR(6-SO4) and [3H]ATalR(6-SO4). |
T15 | 1763-1947 | Sentence | denotes | Digestion of both GlcA(2-SO4)-[3H]AManR(6-SO4) and GlcA(2-SO4)-[3H]ATalR(6-SO4) was inhibited by 35SO2-4 and was arrested at the monosulfated disaccharide stage by 1,4-saccharolactone. |
T16 | 1948-2002 | Sentence | denotes | The glucurono-2-sulfatase exhibited a pH optimum of 4. |
T17 | 2003-2151 | Sentence | denotes | The results indicate that there exists a separate sulfatase for the removal of sulfate substituents from C-2 of GlcA residues in glycosaminoglycans. |
GlyCosmos6-Glycan-Motif-Structure
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 165-172 | https://glytoucan.org/Structures/Glycans/G00021MO | denotes | heparin |
T2 | 165-172 | https://glytoucan.org/Structures/Glycans/G54161DR | denotes | heparin |
T3 | 367-374 | https://glytoucan.org/Structures/Glycans/G00021MO | denotes | heparin |
T4 | 367-374 | https://glytoucan.org/Structures/Glycans/G54161DR | denotes | heparin |
T5 | 394-415 | https://glytoucan.org/Structures/Glycans/G34992DF | denotes | chondroitin 6-sulfate |
T6 | 394-405 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
T7 | 652-659 | https://glytoucan.org/Structures/Glycans/G00021MO | denotes | heparin |
T8 | 652-659 | https://glytoucan.org/Structures/Glycans/G54161DR | denotes | heparin |
T9 | 664-675 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
T10 | 843-850 | https://glytoucan.org/Structures/Glycans/G00021MO | denotes | heparin |
T11 | 843-850 | https://glytoucan.org/Structures/Glycans/G54161DR | denotes | heparin |
T12 | 889-900 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
Glycosmos6-MAT
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 1111-1117 | http://purl.obolibrary.org/obo/MAT_0000226 | denotes | embryo |
bionlp-st-pc-2013-training
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 25-50 | Simple_chemical | denotes | glucuronic acid 2-sulfate |
T2 | 63-81 | Simple_chemical | denotes | glycosaminoglycans |
T3 | 105-120 | Simple_chemical | denotes | L-iduronic acid |
T4 | 122-126 | Simple_chemical | denotes | IdoA |
T5 | 165-172 | Simple_chemical | denotes | heparin |
T6 | 187-204 | Simple_chemical | denotes | D-glucuronic acid |
T7 | 206-210 | Simple_chemical | denotes | GlcA |
T8 | 367-374 | Simple_chemical | denotes | heparin |
T9 | 394-415 | Simple_chemical | denotes | chondroitin 6-sulfate |
T10 | 421-433 | Simple_chemical | denotes | nitrous acid |
T11 | 509-520 | Simple_chemical | denotes | 2-O-sulfate |
T12 | 539-543 | Simple_chemical | denotes | GlcA |
T13 | 552-556 | Simple_chemical | denotes | IdoA |
T14 | 608-614 | Simple_chemical | denotes | NaB3H4 |
T15 | 652-659 | Simple_chemical | denotes | heparin |
T16 | 664-683 | Simple_chemical | denotes | chondroitin sulfate |
T17 | 712-734 | Simple_chemical | denotes | 2,5-anhydro-D-mannitol |
T18 | 736-745 | Simple_chemical | denotes | [3H]AManR |
T19 | 751-772 | Simple_chemical | denotes | 2,5-anhydro-D-talitol |
T20 | 774-783 | Simple_chemical | denotes | [3H]ATalR |
T21 | 809-837 | Simple_chemical | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) |
T22 | 843-850 | Simple_chemical | denotes | heparin |
T23 | 855-883 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]ATalR(6-SO4) |
T24 | 889-908 | Simple_chemical | denotes | chondroitin sulfate |
T25 | 946-974 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) |
T26 | 1008-1036 | Simple_chemical | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) |
T27 | 1042-1051 | Simple_chemical | denotes | hydrazine |
T28 | 1070-1079 | Cellular_component | denotes | Lysosomal |
T29 | 1208-1215 | Simple_chemical | denotes | 2-O-SO4 |
T30 | 1237-1248 | Simple_chemical | denotes | uronic acid |
T31 | 1261-1289 | Simple_chemical | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) |
T32 | 1291-1320 | Simple_chemical | denotes | GlcA(2-SO4)-[3H] AManR(6-SO4) |
T33 | 1326-1354 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]ATalR(6-SO4) |
T34 | 1371-1380 | Cellular_component | denotes | lysosomal |
T35 | 1447-1466 | Gene_or_gene_product | denotes | idurono-2-sulfatase |
T36 | 1517-1545 | Simple_chemical | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) |
T37 | 1557-1585 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) |
T38 | 1602-1624 | Simple_chemical | denotes | GlcA-[3H] AManR(6-SO4) |
T39 | 1629-1645 | Simple_chemical | denotes | [3H]AManR(6-SO4) |
T40 | 1674-1702 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]ATalR(6-SO4) |
T41 | 1719-1740 | Simple_chemical | denotes | GlcA-[3H]ATalR(6-SO4) |
T42 | 1745-1761 | Simple_chemical | denotes | [3H]ATalR(6-SO4) |
T43 | 1781-1809 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) |
T44 | 1814-1842 | Simple_chemical | denotes | GlcA(2-SO4)-[3H]ATalR(6-SO4) |
T45 | 1860-1867 | Simple_chemical | denotes | 35SO2-4 |
T46 | 1892-1917 | Simple_chemical | denotes | monosulfated disaccharide |
T47 | 1927-1946 | Simple_chemical | denotes | 1,4-saccharolactone |
T48 | 1952-1973 | Gene_or_gene_product | denotes | glucurono-2-sulfatase |
T49 | 2082-2089 | Simple_chemical | denotes | sulfate |
T50 | 2108-2111 | Simple_chemical | denotes | C-2 |
T51 | 2115-2119 | Simple_chemical | denotes | GlcA |
T52 | 2132-2150 | Simple_chemical | denotes | glycosaminoglycans |
Anatomy-MAT
Id | Subject | Object | Predicate | Lexical cue | mat_id |
---|---|---|---|---|---|
T1 | 1111-1117 | Body_part | denotes | embryo | http://purl.obolibrary.org/obo/MAT_0000226 |
mondo_disease
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T1 | 1468-1485 | Disease | denotes | Hunter's-syndrome | http://purl.obolibrary.org/obo/MONDO_0010674 |
Glycan-GlyCosmos
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 394-415 | Glycan | denotes | chondroitin 6-sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G34992DF |
T2 | 664-675 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
T3 | 889-900 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
GlyCosmos-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
---|---|---|---|---|---|
T1 | 394-415 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin 6-sulfate | http://www.glycoepitope.jp/epitopes/EP0083 |
T2 | 664-675 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
T3 | 889-900 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
GlyCosmos15-MONDO
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T1 | 1468-1485 | Disease | denotes | Hunter's-syndrome | http://purl.obolibrary.org/obo/MONDO_0010674 |
GlyCosmos15-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 1151-1156 | OrganismTaxon | denotes | human | 9606 |
T2 | 1407-1412 | OrganismTaxon | denotes | human | 9606 |
GlyCosmos15-CL
Id | Subject | Object | Predicate | Lexical cue | cl_id |
---|---|---|---|---|---|
T1 | 1118-1130 | Cell | denotes | chondrocytes | http://purl.obolibrary.org/obo/CL:0000138 |
T2 | 1157-1167 | Cell | denotes | fibroblast | http://purl.obolibrary.org/obo/CL:0000057 |
T3 | 1413-1423 | Cell | denotes | fibroblast | http://purl.obolibrary.org/obo/CL:0000057 |
GlyCosmos15-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 1111-1117 | Body_part | denotes | embryo | http://purl.obolibrary.org/obo/UBERON_0000922 |
T2 | 1118-1130 | Body_part | denotes | chondrocytes | http://purl.obolibrary.org/obo/CL_0000138 |
T3 | 1157-1167 | Body_part | denotes | fibroblast | http://purl.obolibrary.org/obo/CL_0000057 |
T4 | 1413-1423 | Body_part | denotes | fibroblast | http://purl.obolibrary.org/obo/CL_0000057 |
GlyCosmos15-MAT
Id | Subject | Object | Predicate | Lexical cue | mat_id |
---|---|---|---|---|---|
T1 | 1111-1117 | Body_part | denotes | embryo | http://purl.obolibrary.org/obo/MAT_0000226 |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-82 | Sentence | denotes | A sulfatase specific for glucuronic acid 2-sulfate residues in glycosaminoglycans. |
T2 | 83-269 | Sentence | denotes | Although 2-O-sulfated L-iduronic acid (IdoA) residues have been known to occur in heparin, 2-O-sulfated D-glucuronic acid (GlcA) residues have been reported only recently (Bienkowski, M. |
T3 | 270-288 | Sentence | denotes | J., and Conrad, H. |
T4 | 289-301 | Sentence | denotes | E. (1985) J. |
T5 | 302-307 | Sentence | denotes | Biol. |
T6 | 308-313 | Sentence | denotes | Chem. |
T7 | 314-328 | Sentence | denotes | 250, 356-365). |
T8 | 329-566 | Sentence | denotes | Disaccharides prepared by cleavage of heparin and N-deacetylated chondroitin 6-sulfate with nitrous acid were used to demonstrate a new sulfatase that catalyzed the removal of the 2-O-sulfate substituents from GlcA but not IdoA residues. |
T9 | 567-808 | Sentence | denotes | The deamination products were labeled by NaB3H4 reduction to give disaccharides from heparin and chondroitin sulfate which had reducing terminal 2,5-anhydro-D-mannitol ([3H]AManR) and 2,5-anhydro-D-talitol ([3H]ATalR) residues, respectively. |
T10 | 809-945 | Sentence | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) from heparin and GlcA(2-SO4)-[3H]ATalR(6-SO4) from chondroitin sulfate were purified for use as substrates. |
T11 | 946-1069 | Sentence | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) was prepared by epimerization of IdoA(2-SO4)-[3H]AManR(6-SO4) with hydrazine at 100 degrees C. |
T12 | 1070-1355 | Sentence | denotes | Lysosomal enzyme preparations from chick embryo chondrocytes and from two normal human fibroblast cell lines catalyzed the removal of the 2-O-SO4 substituent from the uronic acid residues of IdoA(2-SO4)-[3H]AManR(6-SO4), GlcA(2-SO4)-[3H] AManR(6-SO4), and GlcA(2-SO4)-[3H]ATalR(6-SO4). |
T13 | 1356-1646 | Sentence | denotes | In contrast, a lysosomal enzyme preparation from a human fibroblast cell line deficient in idurono-2-sulfatase (Hunter's-syndrome), which had no activity on the IdoA(2-SO4)-[3H]AManR(6-SO4), converted GlcA(2-SO4)-[3H]AManR(6-SO4) to a mixture of GlcA-[3H] AManR(6-SO4) and [3H]AManR(6-SO4). |
T14 | 1647-1762 | Sentence | denotes | This enzyme also converted GlcA(2-SO4)-[3H]ATalR(6-SO4) to a mixture of GlcA-[3H]ATalR(6-SO4) and [3H]ATalR(6-SO4). |
T15 | 1763-1947 | Sentence | denotes | Digestion of both GlcA(2-SO4)-[3H]AManR(6-SO4) and GlcA(2-SO4)-[3H]ATalR(6-SO4) was inhibited by 35SO2-4 and was arrested at the monosulfated disaccharide stage by 1,4-saccharolactone. |
T16 | 1948-2002 | Sentence | denotes | The glucurono-2-sulfatase exhibited a pH optimum of 4. |
T17 | 2003-2151 | Sentence | denotes | The results indicate that there exists a separate sulfatase for the removal of sulfate substituents from C-2 of GlcA residues in glycosaminoglycans. |
T1 | 0-82 | Sentence | denotes | A sulfatase specific for glucuronic acid 2-sulfate residues in glycosaminoglycans. |
T2 | 83-269 | Sentence | denotes | Although 2-O-sulfated L-iduronic acid (IdoA) residues have been known to occur in heparin, 2-O-sulfated D-glucuronic acid (GlcA) residues have been reported only recently (Bienkowski, M. |
T3 | 270-288 | Sentence | denotes | J., and Conrad, H. |
T4 | 289-301 | Sentence | denotes | E. (1985) J. |
T5 | 302-307 | Sentence | denotes | Biol. |
T6 | 308-313 | Sentence | denotes | Chem. |
T7 | 314-328 | Sentence | denotes | 250, 356-365). |
T8 | 329-566 | Sentence | denotes | Disaccharides prepared by cleavage of heparin and N-deacetylated chondroitin 6-sulfate with nitrous acid were used to demonstrate a new sulfatase that catalyzed the removal of the 2-O-sulfate substituents from GlcA but not IdoA residues. |
T9 | 567-808 | Sentence | denotes | The deamination products were labeled by NaB3H4 reduction to give disaccharides from heparin and chondroitin sulfate which had reducing terminal 2,5-anhydro-D-mannitol ([3H]AManR) and 2,5-anhydro-D-talitol ([3H]ATalR) residues, respectively. |
T10 | 809-945 | Sentence | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) from heparin and GlcA(2-SO4)-[3H]ATalR(6-SO4) from chondroitin sulfate were purified for use as substrates. |
T11 | 946-1069 | Sentence | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) was prepared by epimerization of IdoA(2-SO4)-[3H]AManR(6-SO4) with hydrazine at 100 degrees C. |
T12 | 1070-1355 | Sentence | denotes | Lysosomal enzyme preparations from chick embryo chondrocytes and from two normal human fibroblast cell lines catalyzed the removal of the 2-O-SO4 substituent from the uronic acid residues of IdoA(2-SO4)-[3H]AManR(6-SO4), GlcA(2-SO4)-[3H] AManR(6-SO4), and GlcA(2-SO4)-[3H]ATalR(6-SO4). |
T13 | 1356-1646 | Sentence | denotes | In contrast, a lysosomal enzyme preparation from a human fibroblast cell line deficient in idurono-2-sulfatase (Hunter's-syndrome), which had no activity on the IdoA(2-SO4)-[3H]AManR(6-SO4), converted GlcA(2-SO4)-[3H]AManR(6-SO4) to a mixture of GlcA-[3H] AManR(6-SO4) and [3H]AManR(6-SO4). |
T14 | 1647-1762 | Sentence | denotes | This enzyme also converted GlcA(2-SO4)-[3H]ATalR(6-SO4) to a mixture of GlcA-[3H]ATalR(6-SO4) and [3H]ATalR(6-SO4). |
T15 | 1763-1947 | Sentence | denotes | Digestion of both GlcA(2-SO4)-[3H]AManR(6-SO4) and GlcA(2-SO4)-[3H]ATalR(6-SO4) was inhibited by 35SO2-4 and was arrested at the monosulfated disaccharide stage by 1,4-saccharolactone. |
T16 | 1948-2002 | Sentence | denotes | The glucurono-2-sulfatase exhibited a pH optimum of 4. |
T17 | 2003-2151 | Sentence | denotes | The results indicate that there exists a separate sulfatase for the removal of sulfate substituents from C-2 of GlcA residues in glycosaminoglycans. |
GlyCosmos15-Sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-82 | Sentence | denotes | A sulfatase specific for glucuronic acid 2-sulfate residues in glycosaminoglycans. |
T2 | 83-328 | Sentence | denotes | Although 2-O-sulfated L-iduronic acid (IdoA) residues have been known to occur in heparin, 2-O-sulfated D-glucuronic acid (GlcA) residues have been reported only recently (Bienkowski, M. J., and Conrad, H. E. (1985) J. Biol. Chem. 250, 356-365). |
T3 | 329-566 | Sentence | denotes | Disaccharides prepared by cleavage of heparin and N-deacetylated chondroitin 6-sulfate with nitrous acid were used to demonstrate a new sulfatase that catalyzed the removal of the 2-O-sulfate substituents from GlcA but not IdoA residues. |
T4 | 567-808 | Sentence | denotes | The deamination products were labeled by NaB3H4 reduction to give disaccharides from heparin and chondroitin sulfate which had reducing terminal 2,5-anhydro-D-mannitol ([3H]AManR) and 2,5-anhydro-D-talitol ([3H]ATalR) residues, respectively. |
T5 | 809-945 | Sentence | denotes | IdoA(2-SO4)-[3H]AManR(6-SO4) from heparin and GlcA(2-SO4)-[3H]ATalR(6-SO4) from chondroitin sulfate were purified for use as substrates. |
T6 | 946-1355 | Sentence | denotes | GlcA(2-SO4)-[3H]AManR(6-SO4) was prepared by epimerization of IdoA(2-SO4)-[3H]AManR(6-SO4) with hydrazine at 100 degrees C. Lysosomal enzyme preparations from chick embryo chondrocytes and from two normal human fibroblast cell lines catalyzed the removal of the 2-O-SO4 substituent from the uronic acid residues of IdoA(2-SO4)-[3H]AManR(6-SO4), GlcA(2-SO4)-[3H] AManR(6-SO4), and GlcA(2-SO4)-[3H]ATalR(6-SO4). |
T7 | 1356-1646 | Sentence | denotes | In contrast, a lysosomal enzyme preparation from a human fibroblast cell line deficient in idurono-2-sulfatase (Hunter's-syndrome), which had no activity on the IdoA(2-SO4)-[3H]AManR(6-SO4), converted GlcA(2-SO4)-[3H]AManR(6-SO4) to a mixture of GlcA-[3H] AManR(6-SO4) and [3H]AManR(6-SO4). |
T8 | 1647-1762 | Sentence | denotes | This enzyme also converted GlcA(2-SO4)-[3H]ATalR(6-SO4) to a mixture of GlcA-[3H]ATalR(6-SO4) and [3H]ATalR(6-SO4). |
T9 | 1763-1947 | Sentence | denotes | Digestion of both GlcA(2-SO4)-[3H]AManR(6-SO4) and GlcA(2-SO4)-[3H]ATalR(6-SO4) was inhibited by 35SO2-4 and was arrested at the monosulfated disaccharide stage by 1,4-saccharolactone. |
T10 | 1948-2002 | Sentence | denotes | The glucurono-2-sulfatase exhibited a pH optimum of 4. |
T11 | 2003-2151 | Sentence | denotes | The results indicate that there exists a separate sulfatase for the removal of sulfate substituents from C-2 of GlcA residues in glycosaminoglycans. |
GlyCosmos15-Glycan
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 394-415 | Glycan | denotes | chondroitin 6-sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G34992DF |
T2 | 664-675 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
T3 | 889-900 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
GlyCosmos15-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
---|---|---|---|---|---|
T1 | 394-415 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin 6-sulfate | http://www.glycoepitope.jp/epitopes/EP0083 |
T2 | 664-675 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
T3 | 889-900 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 1151-1156 | OrganismTaxon | denotes | human | 9606 |
T2 | 1407-1412 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 1111-1117 | Body_part | denotes | embryo | http://purl.obolibrary.org/obo/UBERON_0000922 |
T2 | 1118-1130 | Body_part | denotes | chondrocytes | http://purl.obolibrary.org/obo/CL_0000138 |
T3 | 1157-1167 | Body_part | denotes | fibroblast | http://purl.obolibrary.org/obo/CL_0000057 |
T4 | 1413-1423 | Body_part | denotes | fibroblast | http://purl.obolibrary.org/obo/CL_0000057 |
CL-cell
Id | Subject | Object | Predicate | Lexical cue | cl_id |
---|---|---|---|---|---|
T1 | 1118-1130 | Cell | denotes | chondrocytes | http://purl.obolibrary.org/obo/CL:0000138 |
T2 | 1157-1167 | Cell | denotes | fibroblast | http://purl.obolibrary.org/obo/CL:0000057 |
T3 | 1413-1423 | Cell | denotes | fibroblast | http://purl.obolibrary.org/obo/CL:0000057 |