PubMed:3990690 JSONTXT

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":233,"end":238},"obj":"gene:4827"},{"id":"T1","span":{"begin":290,"end":303},"obj":"disease:C0004565"},{"id":"T2","span":{"begin":233,"end":238},"obj":"gene:10128"},{"id":"T3","span":{"begin":290,"end":303},"obj":"disease:C0004565"},{"id":"T4","span":{"begin":233,"end":238},"obj":"gene:3572"},{"id":"T5","span":{"begin":290,"end":303},"obj":"disease:C0004565"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"DNA-mediated transfer of human melanoma cell surface glycoprotein gp130: identification of transfectants by erythrocyte rosetting.\nDNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable neomycin resistance gene (aminoglycoside phosphotransferase). Mouse transfectants were identified by a rapid and precise screening method with mouse monoclonal antibodies and erythrocyte rosetting. The frequency of gp130 transfectants was approximately 1 in 2,000 to 5,000 colonies with neo+ cells. Analysis of secondary mouse transfectants has revealed that the transfected gp130 has a molecular weight, isoelectric point, intracellular processing, peptide map, and spatial orientation of surface-exposed epitopes indistinguishable from those seen with gp130 from human melanoma cells. In contrast to primary transfectants, secondary transfectants expressing gp130 lack demonstrable human repetitive sequences."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"3990690-1#40#54#gene19","span":{"begin":171,"end":185},"obj":"gene19"},{"id":"3990690-1#31#39#diseaseC0025202","span":{"begin":162,"end":170},"obj":"diseaseC0025202"}],"relations":[{"id":"40#54#gene1931#39#diseaseC0025202","pred":"associated_with","subj":"3990690-1#40#54#gene19","obj":"3990690-1#31#39#diseaseC0025202"}],"text":"DNA-mediated transfer of human melanoma cell surface glycoprotein gp130: identification of transfectants by erythrocyte rosetting.\nDNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable neomycin resistance gene (aminoglycoside phosphotransferase). Mouse transfectants were identified by a rapid and precise screening method with mouse monoclonal antibodies and erythrocyte rosetting. The frequency of gp130 transfectants was approximately 1 in 2,000 to 5,000 colonies with neo+ cells. Analysis of secondary mouse transfectants has revealed that the transfected gp130 has a molecular weight, isoelectric point, intracellular processing, peptide map, and spatial orientation of surface-exposed epitopes indistinguishable from those seen with gp130 from human melanoma cells. In contrast to primary transfectants, secondary transfectants expressing gp130 lack demonstrable human repetitive sequences."}

{"project":"DisGeNet-2017-sample","denotations":[{"id":"T814","span":{"begin":233,"end":238},"obj":"gene:3572"},{"id":"T815","span":{"begin":290,"end":303},"obj":"disease:C0004565"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T814","obj":"T815"},{"id":"R2","pred":"associated_with","subj":"T814","obj":"T815"},{"id":"R3","pred":"associated_with","subj":"T814","obj":"T815"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"DNA-mediated transfer of human melanoma cell surface glycoprotein gp130: identification of transfectants by erythrocyte rosetting.\nDNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable neomycin resistance gene (aminoglycoside phosphotransferase). Mouse transfectants were identified by a rapid and precise screening method with mouse monoclonal antibodies and erythrocyte rosetting. The frequency of gp130 transfectants was approximately 1 in 2,000 to 5,000 colonies with neo+ cells. Analysis of secondary mouse transfectants has revealed that the transfected gp130 has a molecular weight, isoelectric point, intracellular processing, peptide map, and spatial orientation of surface-exposed epitopes indistinguishable from those seen with gp130 from human melanoma cells. In contrast to primary transfectants, secondary transfectants expressing gp130 lack demonstrable human repetitive sequences."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":130},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":131,"end":391},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":392,"end":527},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":528,"end":628},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":629,"end":916},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":917,"end":1041},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":130},"obj":"Sentence"},{"id":"T2","span":{"begin":131,"end":391},"obj":"Sentence"},{"id":"T3","span":{"begin":392,"end":527},"obj":"Sentence"},{"id":"T4","span":{"begin":528,"end":628},"obj":"Sentence"},{"id":"T5","span":{"begin":629,"end":916},"obj":"Sentence"},{"id":"T6","span":{"begin":917,"end":1041},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"DNA-mediated transfer of human melanoma cell surface glycoprotein gp130: identification of transfectants by erythrocyte rosetting.\nDNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable neomycin resistance gene (aminoglycoside phosphotransferase). Mouse transfectants were identified by a rapid and precise screening method with mouse monoclonal antibodies and erythrocyte rosetting. The frequency of gp130 transfectants was approximately 1 in 2,000 to 5,000 colonies with neo+ cells. Analysis of secondary mouse transfectants has revealed that the transfected gp130 has a molecular weight, isoelectric point, intracellular processing, peptide map, and spatial orientation of surface-exposed epitopes indistinguishable from those seen with gp130 from human melanoma cells. In contrast to primary transfectants, secondary transfectants expressing gp130 lack demonstrable human repetitive sequences."}