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sentences

Id Subject Object Predicate Lexical cue
T1 0-118 Sentence denotes Tripolyphosphate is an alternative phosphodonor of the selective protein phosphorylation of liver microsomal membrane.
T2 119-365 Sentence denotes Two proteins (Mr = 145,000 and Mr = 130,000) of rat liver microsomal membrane are selectively phosphorylated in a characteristic biphasic time course by incubating the membrane with [gamma-32P]ATP in the absence of exogenously added Mg2+ (Lam, K.
T3 366-384 Sentence denotes S., and Kasper, C.
T4 385-397 Sentence denotes B. (1980) J.
T5 398-403 Sentence denotes Biol.
T6 404-409 Sentence denotes Chem.
T7 410-424 Sentence denotes 255, 259-266).
T8 425-570 Sentence denotes This endogenous phosphorylation system was solubilized with Triton X-100 and fractionated by chromatography with DEAE-cellulose and Sepharose 4B.
T9 571-852 Sentence denotes The resulting preparation lacked both ATPase and inorganic pyrophosphatase activity, but retained its original character: the first phase occurred in the presence of ATP but the second phase was initiated after its depletion, implying the presence of a phosphodonor other than ATP.
T10 853-1047 Sentence denotes The putative phosphoryl donors were demonstrated to be ATP in the first phase and in the second phase tripolyphosphate, which is present in [gamma-32P]ATP preparations as a radioactive impurity.
T11 1048-1360 Sentence denotes The latter conclusion was corroborated by results showing that tripolyphosphate purified from a commercial [gamma-32P]ATP and chemically synthesized [32P] tripolyphosphate were both capable of phosphorylating the two proteins and that the unlabeled tripolyphosphate competed effectively against the phosphodonor.
T12 1361-1569 Sentence denotes A rapid dephosphorylation was observed in both phases upon removal of substrates during the reaction, indicating that there is a continuous turnover of the phosphoryl groups being transferred to the proteins.
T13 1570-1761 Sentence denotes The second phase of phosphorylation maintained by the tripolyphosphate was shown to be reversibly inhibited by micromolar levels of ATP, ADP, and nonhydrolyzable analogues of these compounds.
T14 1762-1831 Sentence denotes The implications of this unique phosphorylation system are discussed.
T1 0-118 Sentence denotes Tripolyphosphate is an alternative phosphodonor of the selective protein phosphorylation of liver microsomal membrane.
T2 119-365 Sentence denotes Two proteins (Mr = 145,000 and Mr = 130,000) of rat liver microsomal membrane are selectively phosphorylated in a characteristic biphasic time course by incubating the membrane with [gamma-32P]ATP in the absence of exogenously added Mg2+ (Lam, K.
T3 366-384 Sentence denotes S., and Kasper, C.
T4 385-397 Sentence denotes B. (1980) J.
T5 398-403 Sentence denotes Biol.
T6 404-409 Sentence denotes Chem.
T7 410-424 Sentence denotes 255, 259-266).
T8 425-570 Sentence denotes This endogenous phosphorylation system was solubilized with Triton X-100 and fractionated by chromatography with DEAE-cellulose and Sepharose 4B.
T9 571-852 Sentence denotes The resulting preparation lacked both ATPase and inorganic pyrophosphatase activity, but retained its original character: the first phase occurred in the presence of ATP but the second phase was initiated after its depletion, implying the presence of a phosphodonor other than ATP.
T10 853-1047 Sentence denotes The putative phosphoryl donors were demonstrated to be ATP in the first phase and in the second phase tripolyphosphate, which is present in [gamma-32P]ATP preparations as a radioactive impurity.
T11 1048-1360 Sentence denotes The latter conclusion was corroborated by results showing that tripolyphosphate purified from a commercial [gamma-32P]ATP and chemically synthesized [32P] tripolyphosphate were both capable of phosphorylating the two proteins and that the unlabeled tripolyphosphate competed effectively against the phosphodonor.
T12 1361-1569 Sentence denotes A rapid dephosphorylation was observed in both phases upon removal of substrates during the reaction, indicating that there is a continuous turnover of the phosphoryl groups being transferred to the proteins.
T13 1570-1761 Sentence denotes The second phase of phosphorylation maintained by the tripolyphosphate was shown to be reversibly inhibited by micromolar levels of ATP, ADP, and nonhydrolyzable analogues of these compounds.
T14 1762-1831 Sentence denotes The implications of this unique phosphorylation system are discussed.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 92-97 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver
T2 171-176 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 92-97 Body_part denotes liver http://purl.obolibrary.org/obo/MAT_0000097
T2 171-176 Body_part denotes liver http://purl.obolibrary.org/obo/MAT_0000097

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 167-170 OrganismTaxon denotes rat 10114|10116

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 92-97 Body_part denotes liver http://purl.obolibrary.org/obo/UBERON_0002107
T2 109-117 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T5 171-176 Body_part denotes liver http://purl.obolibrary.org/obo/UBERON_0002107
T6 188-196 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T9 287-295 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158