PubMed:3923186 JSONTXT

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A23187 at a concentration of 10(-7) M did not modify the resting muscle tone, but this concentration did increase the amplitude of the contraction evoked by 20.2 or 128 mM-K+ and markedly inhibited the 10(-7) M-NA or 10 mM-caffeine-induced contraction in Krebs solution. A23187 (10(-7) M) delayed the onset and rising phase of the 10(-5) M-NA-induced contraction with inhibition of the oscillatory contractions. High concentrations of A23187 (over 10(-6) M) produced a large contraction in the presence and a small contraction in the absence of 2.6 mM-Ca2+. These A23187-induced contractions were not inhibited by 10(-7) M-nisoldipine, a Ca2+ antagonist. A23187 (over 10(-6) M) applied for a long period functionally skinned the muscle tissues. However, the Ca2+ sensitivity of the A23187-treated skinned muscles was lower than that of saponin-treated muscles. In saponin-treated skinned muscles, A23187 (below 10(-6) M) had no effect on the pCa-tension relation. After filling the store, A23187 (over 10(-7) M) generated a larger contraction than did caffeine in Ca2+-free solution, in the presence or absence of 5 mM-NaN3. When 10(-7) M-A23187 was applied once for 5 min, subsequently applied caffeine (20 mM), following application of Ca2+, no longer produced contraction of skinned muscle tissues. The present results indicate that low concentrations of A23187 show a selective Ca2+-releasing action on Ca2+ store sites in muscle cells and that high concentrations increase the Ca2+ leakage (influx) and the cell membrane is 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