PubMed:39115362
Annnotations
Glycan-GlyCosmos
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 12-18 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
T2 | 1109-1115 | Glycan | denotes | Neu5Ac | https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR |
T3 | 1195-1201 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
T4 | 1617-1623 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
GlyCosmos15-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 35-41 | OrganismTaxon | denotes | bovine | 9913 |
T2 | 444-450 | OrganismTaxon | denotes | bovine | 9913 |
GlyCosmos15-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 99-108 | Body_part | denotes | colostrum | http://purl.obolibrary.org/obo/UBERON_0001914 |
T2 | 119-123 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
T3 | 176-180 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
T4 | 468-477 | Body_part | denotes | colostrum | http://purl.obolibrary.org/obo/UBERON_0001914 |
T5 | 482-486 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
T6 | 1519-1528 | Body_part | denotes | colostrum | http://purl.obolibrary.org/obo/UBERON_0001914 |
T7 | 1539-1543 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
GlyCosmos15-Sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-124 | Sentence | denotes | LacdiNAc to LacNAc: remodelling of bovine α-lactalbumin N-glycosylation during the transition from colostrum to mature milk. |
T2 | 125-273 | Sentence | denotes | α -Lactalbumin, an abundant protein present in the milk of most mammals, is associated with biological, nutritional and technological functionality. |
T3 | 274-398 | Sentence | denotes | Its sequence presents N-glycosylation motifs, the occupancy of which is species-specific, ranging from no to full occupancy. |
T4 | 399-602 | Sentence | denotes | Here, we investigated the N-glycosylation of bovine α-lactalbumin in colostrum and milk sampled from four individual cows, each at 9 time points starting from the day of calving up to 28.0 d post-partum. |
T5 | 603-824 | Sentence | denotes | Using a glycopeptide-centric mass spectrometry-based glycoproteomics approach, we identified N-glycosylation at both Asn residues found in the canonical Asn-Xxx-Ser/Thr motif, i.e. Asn45 and Asn74 of the secreted protein. |
T6 | 825-965 | Sentence | denotes | We found similar glycan profiles in all four cows, with partial site occupancies, averaging at 35% and 4% for Asn45 and Asn74, respectively. |
T7 | 966-1041 | Sentence | denotes | No substantial changes in occupancy occurred over lactation at either site. |
T8 | 1042-1267 | Sentence | denotes | Fucosylation, sialylation, primarily with N-acetylneuraminic acid (Neu5Ac), and a high ratio of N,N'-diacetyllactosamine (LacdiNAc)/N-acetyllactosamine (LacNAc) motifs were characteristic features of the identified N-glycans. |
T9 | 1268-1677 | Sentence | denotes | While no substantial changes occurred in site occupancy at either site during lactation, the glycoproteoform (i.e. glycosylated form of the protein) profile revealed dynamic changes; the maturation of the α-lactalbumin glycoproteoform repertoire from colostrum to mature milk was marked by substantial increases in neutral glycans and the number of LacNAc motifs per glycan, at the expense of LacdiNAc motifs. |
T10 | 1678-1959 | Sentence | denotes | While the implications of α-lactalbumin N-glycosylation on functionality are still unclear, we speculate that N-glycosylation at Asn74 results in a structurally and functionally different protein, due to competition with the formation of its two intra-molecular disulphide bridges. |
GlyCosmos15-Glycan
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 12-18 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
T2 | 1109-1115 | Glycan | denotes | Neu5Ac | https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR |
T3 | 1195-1201 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
T4 | 1617-1623 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 35-41 | OrganismTaxon | denotes | bovine | 9913 |
T2 | 444-450 | OrganismTaxon | denotes | bovine | 9913 |
Anatomy-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 99-108 | Body_part | denotes | colostrum | http://purl.obolibrary.org/obo/UBERON_0001914 |
T2 | 119-123 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
T3 | 176-180 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
T4 | 468-477 | Body_part | denotes | colostrum | http://purl.obolibrary.org/obo/UBERON_0001914 |
T5 | 482-486 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
T6 | 1519-1528 | Body_part | denotes | colostrum | http://purl.obolibrary.org/obo/UBERON_0001914 |
T7 | 1539-1543 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |