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sentences

Id Subject Object Predicate Lexical cue
T1 0-110 Sentence denotes Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
T2 111-238 Sentence denotes Limited proteolysis has been utilized to study the structural organization of rabbit skeletal muscle myosin light chain kinase.
T3 239-555 Sentence denotes The enzyme (Mr approximately 89,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) consists of an amino-terminal, protease-susceptible region of unidentified function and a carboxyl-terminal, protease-resistant region of Mr approximately 40,000 containing the catalytic and calmodulin-binding domains.
T4 556-737 Sentence denotes Partial digestion with trypsin produced an intermediate 56,000-dalton fragment and a stable 38,000-dalton fragment, both of which were catalytically active and calmodulin-dependent.
T5 738-850 Sentence denotes Chymotryptic digestion yielded three catalytically active fragments of about 37,000, 36,000, and 35,000 daltons.
T6 851-988 Sentence denotes The Mr = 37,000 fragment was calmodulin-dependent with an apparent affinity equivalent to that of the native enzyme (approximately 1 nM).
T7 989-1103 Sentence denotes The 36,000-dalton fragment was also calmodulin-dependent but had a approximately 200-fold lower apparent affinity.
T8 1104-1156 Sentence denotes The Mr = 35,000 fragment was calmodulin-independent.
T9 1157-1221 Sentence denotes These three chymotryptic fragments, had identical amino termini.
T10 1222-1440 Sentence denotes Nineteen residues were missing from the carboxyl terminus of the calmodulin-independent chymotryptic fragment whereas only 8 or 9 carboxyl-terminal residues were missing from the calmodulin-dependent tryptic fragments.
T11 1441-1617 Sentence denotes These results suggest that the 11-residue sequence (IAVSAANRFKK) in the carboxyl-terminal region of myosin light chain kinase contributes directly to the binding of calmodulin.
T12 1618-1672 Sentence denotes This conclusion is in accord with data (Blumenthal, D.
T13 1673-1699 Sentence denotes K., Takio, K., Edelman, A.
T14 1700-1742 Sentence denotes M., Charbonneau, H., Titani, K., Walsh, K.
T15 1743-1760 Sentence denotes A., and Krebs, E.
T16 1761-1776 Sentence denotes G. (1985) Proc.
T17 1777-1782 Sentence denotes Natl.
T18 1783-1788 Sentence denotes Acad.
T19 1789-1793 Sentence denotes Sci.
T20 1794-1796 Sentence denotes U.
T21 1797-1799 Sentence denotes S.
T22 1800-1802 Sentence denotes A.
T23 1803-2182 Sentence denotes 82, 3187-3191) that the carboxyl-terminal, 27-residue CNBr peptide of the native enzyme shows Ca2+-dependent, high affinity binding to calmodulin and that similar calmodulin-binding activity, although detectable in unfractionated CNBr digests of calmodulin-dependent enzyme forms, is much reduced in a CNBr digest of the calmodulin-independent, Mr = 35,000 chymotryptic fragment.
T1 0-110 Sentence denotes Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
T2 111-238 Sentence denotes Limited proteolysis has been utilized to study the structural organization of rabbit skeletal muscle myosin light chain kinase.
T3 239-555 Sentence denotes The enzyme (Mr approximately 89,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) consists of an amino-terminal, protease-susceptible region of unidentified function and a carboxyl-terminal, protease-resistant region of Mr approximately 40,000 containing the catalytic and calmodulin-binding domains.
T4 556-737 Sentence denotes Partial digestion with trypsin produced an intermediate 56,000-dalton fragment and a stable 38,000-dalton fragment, both of which were catalytically active and calmodulin-dependent.
T5 738-850 Sentence denotes Chymotryptic digestion yielded three catalytically active fragments of about 37,000, 36,000, and 35,000 daltons.
T6 851-988 Sentence denotes The Mr = 37,000 fragment was calmodulin-dependent with an apparent affinity equivalent to that of the native enzyme (approximately 1 nM).
T7 989-1103 Sentence denotes The 36,000-dalton fragment was also calmodulin-dependent but had a approximately 200-fold lower apparent affinity.
T8 1104-1156 Sentence denotes The Mr = 35,000 fragment was calmodulin-independent.
T9 1157-1221 Sentence denotes These three chymotryptic fragments, had identical amino termini.
T10 1222-1440 Sentence denotes Nineteen residues were missing from the carboxyl terminus of the calmodulin-independent chymotryptic fragment whereas only 8 or 9 carboxyl-terminal residues were missing from the calmodulin-dependent tryptic fragments.
T11 1441-1617 Sentence denotes These results suggest that the 11-residue sequence (IAVSAANRFKK) in the carboxyl-terminal region of myosin light chain kinase contributes directly to the binding of calmodulin.
T12 1618-1672 Sentence denotes This conclusion is in accord with data (Blumenthal, D.
T13 1673-1699 Sentence denotes K., Takio, K., Edelman, A.
T14 1700-1742 Sentence denotes M., Charbonneau, H., Titani, K., Walsh, K.
T15 1743-1760 Sentence denotes A., and Krebs, E.
T16 1761-1776 Sentence denotes G. (1985) Proc.
T17 1777-1782 Sentence denotes Natl.
T18 1783-1788 Sentence denotes Acad.
T19 1789-1793 Sentence denotes Sci.
T20 1794-1796 Sentence denotes U.
T21 1797-1799 Sentence denotes S.
T22 1800-1802 Sentence denotes A.
T23 1803-2182 Sentence denotes 82, 3187-3191) that the carboxyl-terminal, 27-residue CNBr peptide of the native enzyme shows Ca2+-dependent, high affinity binding to calmodulin and that similar calmodulin-binding activity, although detectable in unfractionated CNBr digests of calmodulin-dependent enzyme forms, is much reduced in a CNBr digest of the calmodulin-independent, Mr = 35,000 chymotryptic fragment.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 68-83 http://purl.obolibrary.org/obo/MAT_0000302 denotes skeletal muscle
T2 77-83 http://purl.obolibrary.org/obo/MAT_0000025 denotes muscle
T3 196-211 http://purl.obolibrary.org/obo/MAT_0000302 denotes skeletal muscle
T4 205-211 http://purl.obolibrary.org/obo/MAT_0000025 denotes muscle

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 68-83 Body_part denotes skeletal muscle http://purl.obolibrary.org/obo/MAT_0000302
T2 196-211 Body_part denotes skeletal muscle http://purl.obolibrary.org/obo/MAT_0000302

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 68-83 Body_part denotes skeletal muscle http://purl.obolibrary.org/obo/UBERON_0001134|http://purl.obolibrary.org/obo/UBERON_0014892|http://purl.obolibrary.org/obo/UBERON_0014895
T4 196-211 Body_part denotes skeletal muscle http://purl.obolibrary.org/obo/UBERON_0001134|http://purl.obolibrary.org/obo/UBERON_0014892|http://purl.obolibrary.org/obo/UBERON_0014895