PubMed:37985674 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/37985674","sourcedb":"PubMed","sourceid":"37985674","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/37985674","text":"Use of a point-of-care test to rapidly assess levels of SARS-CoV-2 nasal neutralising antibodies in vaccines and breakthrough infected individuals.\nDespite SARS-CoV-2 vaccines eliciting systemic neutralising antibodies (nAbs), breakthrough infections still regularly occur. Infection helps to generate mucosal immunity, possibly reducing disease transmission. Monitoring mucosal nAbs is predominantly restricted to lab-based assays, which have limited application to the public. In this multi-site study, we used lateral-flow surrogate neutralisation tests to measure mucosal and systemic nAbs in vaccinated and breakthrough infected individuals in Australia and Singapore. Using three lateral flow assays to detect SARS-CoV-2 nAbs, we demonstrated that nasal mucosal nAbs were present in 71.4 (95% CI 56.3-82.9%) to 85.7% (95% CI 71.8-93.7%) of individuals with breakthrough infection (positivity rate was dependent upon the type of test), whereas only 20.7 (95% CI 17.1-49.4%) to 34.5% (95% CI 19.8-52.7%) of vaccinated individuals without breakthrough infection had detectible nasal mucosal nAbs. Of the individuals with breakthrough infection, collective mucosal anti-S antibody detection in confirmatory assays was 92.9% (95% CI 80.3-98.2%) of samples, while 72.4% (95% CI 54.1-85.5%) of the vaccinated individuals who had not experienced a breakthrough infection were positive to anti-S antibody. All breakthrough infected individuals produced systemic anti-N antibodies; however, these antibodies were not detected in the nasal cavity. Mucosal immunity is likely to play a role in limiting the transmission of SARS-CoV-2 and lateral flow neutralisation tests provide a rapid readout of mucosal nAbs at the point-of-care.","tracks":[{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":56,"end":66},"obj":"Disease"},{"id":"T2","span":{"begin":156,"end":166},"obj":"Disease"},{"id":"T3","span":{"begin":240,"end":250},"obj":"Disease"},{"id":"T4","span":{"begin":274,"end":283},"obj":"Disease"},{"id":"T5","span":{"begin":716,"end":726},"obj":"Disease"},{"id":"T6","span":{"begin":876,"end":885},"obj":"Disease"},{"id":"T7","span":{"begin":1055,"end":1064},"obj":"Disease"},{"id":"T8","span":{"begin":1137,"end":1146},"obj":"Disease"},{"id":"T9","span":{"begin":1359,"end":1368},"obj":"Disease"},{"id":"T10","span":{"begin":1617,"end":1627},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"},{"subj":"T4","pred":"source","obj":"mondo_disease"},{"subj":"T5","pred":"source","obj":"mondo_disease"},{"subj":"T6","pred":"source","obj":"mondo_disease"},{"subj":"T7","pred":"source","obj":"mondo_disease"},{"subj":"T8","pred":"source","obj":"mondo_disease"},{"subj":"T9","pred":"source","obj":"mondo_disease"},{"subj":"T10","pred":"source","obj":"mondo_disease"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":513,"end":520},"obj":"Body_part"},{"id":"T2","span":{"begin":686,"end":693},"obj":"Body_part"},{"id":"T3","span":{"begin":1632,"end":1639},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000488"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000488"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000488"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T2","pred":"source","obj":"Anatomy-MAT"},{"subj":"T3","pred":"source","obj":"Anatomy-MAT"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":56,"end":64},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":156,"end":164},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":716,"end":724},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1617,"end":1625},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"694009"},{"id":"A2","pred":"db_id","subj":"T2","obj":"694009"},{"id":"A3","pred":"db_id","subj":"T3","obj":"694009"},{"id":"A4","pred":"db_id","subj":"T4","obj":"694009"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1529,"end":1541},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001707"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"mondo_disease","color":"#b593ec","default":true},{"id":"Anatomy-MAT","color":"#93ec9b"},{"id":"NCBITAXON","color":"#ec93a4"},{"id":"Anatomy-UBERON","color":"#93beec"}]}]}}