PubMed:3756946 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3756946","sourcedb":"PubMed","sourceid":"3756946","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/3756946","text":"Methylation analysis of the heptose/3-deoxy-D-manno-2-octulosonic acid region (inner core) of the lipopolysaccharide from Salmonella minnesota rough mutants.\nA modified methylation analysis is described which allows the elucidation of the structure of the inner core region [heptose/3-deoxy-D-manno-2-octulosonic acid (KDO)] of enterobacterial lipopolysaccharides (LPS) of Salmonella minnesota rough mutants (Re, strain R595; and Rd2P-, strain R4). Methylation, carboxyl-reduction, remethylation, hydrolysis, carbonyl-reduction, and acetylation of the Re-mutant LPS yielded the 2,6-di-O-acetyl and 2,4,6-tri-O-acetyl derivatives of partially methylated 3-deoxyoctitol in equimolar amounts, indicating the presence of a terminal and a 4-linked pyranosidic KDO residue. For Rd2P- LPS, the hydrolysis step involved 0.1M trifluoroacetic acid at 100 degrees for 1 h which cleaved ketosidic linkages, and the final products included the foregoing acetyl derivatives in the molar ratio of 1:02 and a partially methylated and acetylated 3-deoxyoctitol derivative which was substituted at O-5 by a methylated heptopyranosyl residue. Trideuteriomethylation of the latter product followed by methanolysis and acetylation gave 5-O-acetyl-3-deoxy-1,7,8-tri-O-methyl-2,4,6-tri-O-trideuteriomethyl++ +-D- glycero-D-talo/galacto-octitol and 1,5-di-O-acetyl-2,3,4,6,7-penta-O-methyl-L-glycero-D-manno-heptitol++ +. These results prove the presence of a (2----4)-linked KDO disaccharide in Re LPS and show that the core region of Rd2P- LPS contains a terminal alpha-L-glycero-D-manno-heptopyranosyl group and a non-substituted, a 4-O-, and a 4,5-di-O-substituted pyranosidic KDO residue in the molar ratios 1:1:0.2:1.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":157},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":158,"end":448},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":449,"end":767},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":768,"end":1123},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":1124,"end":1397},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1398,"end":1699},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":157},"obj":"Sentence"},{"id":"T2","span":{"begin":158,"end":448},"obj":"Sentence"},{"id":"T3","span":{"begin":449,"end":767},"obj":"Sentence"},{"id":"T4","span":{"begin":768,"end":1123},"obj":"Sentence"},{"id":"T5","span":{"begin":1124,"end":1397},"obj":"Sentence"},{"id":"T6","span":{"begin":1398,"end":1699},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":122,"end":132},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":373,"end":383},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"590"},{"id":"A2","pred":"db_id","subj":"T2","obj":"590"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":967,"end":972},"obj":"Body_part"},{"id":"T2","span":{"begin":1676,"end":1681},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0003655"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0003655"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93c2ec","default":true},{"id":"NCBITAXON","color":"#dcec93"},{"id":"Anatomy-UBERON","color":"#e293ec"}]}]}}