PubMed:37276352
Annnotations
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 587-599 | Cell | denotes | immune cells | http://purl.obolibrary.org/obo/CL:0000738 |
| T2 | 708-715 | Cell | denotes | T cells | http://purl.obolibrary.org/obo/CL:0000084 |
| T3 | 820-842 | Cell | denotes | activated CD8+ T cells | http://purl.obolibrary.org/obo/CL:0001049 |
| T4 | 861-866 | Cell | denotes | basal | http://purl.obolibrary.org/obo/CL:0002324 |
| T5 | 890-897 | Cell | denotes | T cells | http://purl.obolibrary.org/obo/CL:0000084 |
| T6 | 923-937 | Cell | denotes | memory T cells | http://purl.obolibrary.org/obo/CL:0000813 |
| T7 | 1048-1055 | Cell | denotes | T cells | http://purl.obolibrary.org/obo/CL:0000084 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 30-36 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T2 | 458-464 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T3 | 478-484 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T4 | 676-682 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T5 | 781-787 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T6 | 971-977 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
GlyCosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 587-599 | Cell | denotes | immune cells | http://purl.obolibrary.org/obo/CL:0000738 |
| T2 | 708-715 | Cell | denotes | T cells | http://purl.obolibrary.org/obo/CL:0000084 |
| T3 | 820-842 | Cell | denotes | activated CD8+ T cells | http://purl.obolibrary.org/obo/CL:0001049 |
| T4 | 861-866 | Cell | denotes | basal | http://purl.obolibrary.org/obo/CL:0002324 |
| T5 | 890-897 | Cell | denotes | T cells | http://purl.obolibrary.org/obo/CL:0000084 |
| T6 | 923-937 | Cell | denotes | memory T cells | http://purl.obolibrary.org/obo/CL:0000813 |
| T7 | 1048-1055 | Cell | denotes | T cells | http://purl.obolibrary.org/obo/CL:0000084 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 587-599 | Body_part | denotes | immune cells | http://purl.obolibrary.org/obo/CL_0000738 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-141 | Sentence | denotes | Chemoenzymatic Measurement of LacNAc in Single-Cell Multiomics Reveals It as a Cell-Surface Indicator of Glycolytic Activity of CD8+ T Cells. |
| T2 | 142-363 | Sentence | denotes | Despite the rich information about the physiological state of a cell encoded in the dynamic changes of cell-surface glycans, chemical methods to capture specific glycan epitopes at the single-cell level are quite limited. |
| T3 | 364-513 | Sentence | denotes | Here, we report a chemoenzymatic method for the single-cell detection of N-acetyllactosamine (LacNAc) by labeling LacNAc with a specific DNA barcode. |
| T4 | 514-652 | Sentence | denotes | The chemoenzymatic labeling does not alter the transcriptional status of immune cells and is compatible with multiple scRNA-seq platforms. |
| T5 | 653-939 | Sentence | denotes | Integrated analysis of LacNAc and the transcriptome of T cells at the single-cell level reveals that the amount of cell-surface LacNAc is significantly upregulated in activated CD8+ T cells but maintained at basal levels in resting CD8+ T cells (i.e., naive and central memory T cells). |
| T6 | 940-1079 | Sentence | denotes | Further analysis confirms that LacNAc levels are positively correlated with the glycolytic activity of CD8+ T cells during differentiation. |
| T7 | 1080-1421 | Sentence | denotes | Taken together, our study demonstrates the feasibility of the chemoenzymatic detection of cell-surface glycan in single-cell RNA sequencing-based multiomics with TCR sequence and cell-surface epitope information (i.e., scTCR and CITE-seq), and provides a new way to characterize the biological role of glycan in diverse physiological states. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 30-36 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T2 | 458-464 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T3 | 478-484 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T4 | 676-682 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T5 | 781-787 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
| T6 | 971-977 | Glycan | denotes | LacNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G66213AR |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 587-599 | Body_part | denotes | immune cells | http://purl.obolibrary.org/obo/CL_0000738 |