PubMed:3690570 JSONTXT

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G22535ZZ"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G22535ZZ"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"https://glytoucan.org/Structures/Glycans/G22535ZZ"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"https://glytoucan.org/Structures/Glycans/G22535ZZ"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":91,"end":322},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":323,"end":437},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":438,"end":631},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":632,"end":896},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":897,"end":1132},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1133,"end":1359},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":322},"obj":"Sentence"},{"id":"T3","span":{"begin":323,"end":437},"obj":"Sentence"},{"id":"T4","span":{"begin":438,"end":631},"obj":"Sentence"},{"id":"T5","span":{"begin":632,"end":896},"obj":"Sentence"},{"id":"T6","span":{"begin":897,"end":1132},"obj":"Sentence"},{"id":"T7","span":{"begin":1133,"end":1359},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"http://www.glycoepitope.jp/epitopes/EP0508"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"http://www.glycoepitope.jp/epitopes/EP0508"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"Glycan"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G22535ZZ"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G22535ZZ"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0508"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0508"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"Glycan"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G22535ZZ"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G22535ZZ"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":1264,"end":1272},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos15-Taxon","denotations":[{"id":"T1","span":{"begin":62,"end":80},"obj":"Organism"},{"id":"T2","span":{"begin":191,"end":209},"obj":"Organism"},{"id":"T3","span":{"begin":738,"end":741},"obj":"Organism"},{"id":"T4","span":{"begin":824,"end":827},"obj":"Organism"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"55605"},{"id":"A2","pred":"db_id","subj":"T2","obj":"55605"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9225"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9225"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":322},"obj":"Sentence"},{"id":"T3","span":{"begin":323,"end":437},"obj":"Sentence"},{"id":"T4","span":{"begin":438,"end":896},"obj":"Sentence"},{"id":"T5","span":{"begin":897,"end":1359},"obj":"Sentence"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":306,"end":314},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":1242,"end":1250},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0508"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0508"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"GlyCosmos15-FMA","denotations":[{"id":"T1","span":{"begin":1264,"end":1272},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"FMA:13478"}],"namespaces":[{"prefix":"FMA","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":62,"end":80},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":191,"end":209},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":738,"end":741},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":824,"end":827},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"55605"},{"id":"A2","pred":"db_id","subj":"T2","obj":"55605"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9225"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9225"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1264,"end":1272},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"}],"text":"Unit structure of the anti-complementary arabinogalactan from Angelica acutiloba Kitagawa.\nMild acid hydrolysis of an anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, gave one neutral (N-I) and two acidic arabinogalactans (A-I and A-II) and one neutral arabinan (N-II). Likewise, the product (AF-AGIIb-1) of digestion with exo-alpha-L-arabinofuranosidase gave four carbohydrate units. Methylation analysis showed that N-I was a (1----6)-linked galactan with unbranched short side-chains of Araf attached at position 3 and that A-I and A-II contained, in addition, 4-linked Galp. Methylation analysis and oligosaccharide analysis showed that A-I and A-II also contained highly branched Ara chains possessing Araf side-chains attached at positions 3 of some 4- or 5-linked Ara and that a small proportion of Arap was present in each acidic unit. Base-catalysed beta-elimination and oligosaccharide analysis indicated that A-1 and A-II also contained a rhamnogalacturonan moiety in which 2,4-disubstituted Rha residues were attached to 4-substituted GalpA through position 2 of Rha. Methylation analysis, 1H- and 13C-n.m.r. studies, and enzymic hydrolysis showed N-II to be a highly branched arabinan containing a backbone of (1----5)-linked alpha-L-Araf with alpha-L-Araf side-chains attached to positions 3."}