| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-39 |
Sentence |
denotes |
Biosynthesis of chick type VI collagen. |
| T2 |
40-43 |
Sentence |
denotes |
II. |
| T3 |
44-108 |
Sentence |
denotes |
Processing and secretion in fibroblasts and smooth muscle cells. |
| T4 |
109-224 |
Sentence |
denotes |
The biosynthesis of type VI collagen was studied in "matrix-free" chick embryo smooth muscle cells and fibroblasts. |
| T5 |
225-371 |
Sentence |
denotes |
Omission of ascorbate from the culture affected to a great extent the secretion in fibroblasts but had a very minor effect on smooth muscle cells. |
| T6 |
372-813 |
Sentence |
denotes |
Quantitative analysis of the secretion process in continuous time course and in pulse-chase experiments confirmed that fibroblasts and smooth muscle cells secreted type VI collagen with the same chain composition but with different kinetics: after 4 h of chase more than 60% of the labeled type VI collagen was present in the culture medium of fibroblasts, whereas at the same time interval less than 25% was secreted by smooth muscle cells. |
| T7 |
814-928 |
Sentence |
denotes |
The different kinetics depends on intrinsic properties of the cells, since it was detected also in adherent cells. |
| T8 |
929-1109 |
Sentence |
denotes |
However, even in fibroblasts, secretion of type VI collagen was much slower than secretion of fibronectin, of which more than 50% was already in the cell medium after 1 h of chase. |
| T9 |
1110-1274 |
Sentence |
denotes |
Treatment of the cells with inhibitors of hydroxylation and glycosylation caused a shift in mobility that revealed a size heterogeneity in the Mr = 260,000 subunit. |
| T10 |
1275-1398 |
Sentence |
denotes |
No evidence of processing was observed in chick cells for any of the subunits that were synthesized and secreted uncleaved. |
| T11 |
1399-1613 |
Sentence |
denotes |
In addition, after several days of chase the Mr of the subunits of type VI collagen isolated from the matrix remained unchanged, thus excluding that in the chick even a partial or incomplete processing takes place. |
| T1 |
0-39 |
Sentence |
denotes |
Biosynthesis of chick type VI collagen. |
| T2 |
40-43 |
Sentence |
denotes |
II. |
| T3 |
44-108 |
Sentence |
denotes |
Processing and secretion in fibroblasts and smooth muscle cells. |
| T4 |
109-224 |
Sentence |
denotes |
The biosynthesis of type VI collagen was studied in "matrix-free" chick embryo smooth muscle cells and fibroblasts. |
| T5 |
225-371 |
Sentence |
denotes |
Omission of ascorbate from the culture affected to a great extent the secretion in fibroblasts but had a very minor effect on smooth muscle cells. |
| T6 |
372-813 |
Sentence |
denotes |
Quantitative analysis of the secretion process in continuous time course and in pulse-chase experiments confirmed that fibroblasts and smooth muscle cells secreted type VI collagen with the same chain composition but with different kinetics: after 4 h of chase more than 60% of the labeled type VI collagen was present in the culture medium of fibroblasts, whereas at the same time interval less than 25% was secreted by smooth muscle cells. |
| T7 |
814-928 |
Sentence |
denotes |
The different kinetics depends on intrinsic properties of the cells, since it was detected also in adherent cells. |
| T8 |
929-1109 |
Sentence |
denotes |
However, even in fibroblasts, secretion of type VI collagen was much slower than secretion of fibronectin, of which more than 50% was already in the cell medium after 1 h of chase. |
| T9 |
1110-1274 |
Sentence |
denotes |
Treatment of the cells with inhibitors of hydroxylation and glycosylation caused a shift in mobility that revealed a size heterogeneity in the Mr = 260,000 subunit. |
| T10 |
1275-1398 |
Sentence |
denotes |
No evidence of processing was observed in chick cells for any of the subunits that were synthesized and secreted uncleaved. |
| T11 |
1399-1613 |
Sentence |
denotes |
In addition, after several days of chase the Mr of the subunits of type VI collagen isolated from the matrix remained unchanged, thus excluding that in the chick even a partial or incomplete processing takes place. |